Poll (NM_001014168) Rat Tagged ORF Clone

CAT#: RR211260

Poll (Myc-DDK-tagged ORF) - Rat polymerase (DNA directed), lambda (Poll), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Poll
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211260 representing NM_001014168
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCCCCAGGGCATCCTGAAGGCATTTCCCAAGCGAAAGAAAATTCATGCAGATCCATCATCAAACG
  CACTTGCAAAGATTCCCAAAAGGGAGGCAGGAGATGCTAGAGGATGGCTGAGCTCCCTGAGGGCCCACAT
  TATGCCCACTGGCATTGGGCGAGCCCGGGCTGAACTCTTTGAGAAGCAGATTATCCAGCATGGTGGCCAG
  GTGTGCTCTGCCCAGGCCCCAGGAGTCACTCACATTGTGGTGGATGAAGGCATGGACTATGAACGGGCTC
  TCCGGCTCCTCAGACTGCCCCAGCTGCCCCCTGGTGCTCAGCTGGTGAAGTCAGCCTGGCTGAGCTTGTG
  TCTACAGGAGAAAAAGCTGACAGACACGGACGGATTCAGCCTTTCCAGCCCTAAGAGGTCCTTGAATGAA
  CCACAGCCCAGCAAGTCAGGCCAAGATGCTTCTGCTCCTGGCACTCAGGGGGTTCTACCCAGGACAACGC
  TCTCTCTTTCCCCTCCTTGTACCAGAGCTGTATCTCCTCCCCCAAAGGCAGAAAAGCCACCAAAAACCCA
  AACCCAGCTCAGCTCAGAGGATGAAGCCAGTGATGGGGAAGGGCCCCAGGTTAGCTCAGCAGATTTGCAA
  GCCTTGATCAGTGGGCACTACCCCACTCCCCCTGGGGAAGATGGTGGGCCTGACCCAGCCCCAGAAGCTC
  TGGGTAAGTGGGTCTGTGCACAGCCCTCAAGCCAGAAGGCAACTAATTACAACCTGCACATCACAGAGAA
  GCTCGAAGTGCTGGCTAAAGCCTACAATGTCCAGGGAGACAAGTGGAGGGCTCTGGGCTATGCCAAGGCC
  ATCAACGCCCTCAAGAGCTTCCACAAGCCTGTCAGTTCCTACCAGGAGGCCTGTAGCATCCCAGGAGTTG
  GCAGGCGAATGGCAGAGAAGGTCATGGAGATCCTGGAGAGTGGGCATCTGCGGAAGCTGGACCACATCAG
  CGACAGCGTGCCTGTCTTAGAGCTCTTCTCCAACATCTGGGGAGCCGGGACGAAGACTGCCCAGATGTGG
  TACCATCAGGGCTTCCGAAGCCTAGAAGATATCCGAGGCCTGGCCTCCCTGACCGCTCAGCAGGCCATTG
  GCTTGAAGCACTATGATGACTTCCTGGACCGCATGCCCAGGGAGGAGGCTGCAGAAATTGAGCAGATGGT
  CCGAGTATCAGCCCAGGCCTTCAACCCTGGGCTGCTGTGTGTGGCCTGTGGCTCTTTCCGTCGAGGGAAG
  GTGACCTGTGGGGATGTAGATGTACTTATTACTCACCCCGACGGCCGGTCCCACCAGGGCATCTTCAGCC
  CACTCCTCGACAGCCTTCGGCAGCAAGGGTTCCTCACAGATGACTTGGTGAGCCAGGAGGAGAATGGCCA
  GCAGCAGAAATACCTGGGTGTGTGCCGGCTCCCAGGGGCCGGGCAGCGCCACCGGCGACTGGACATCATC
  GTGGTGCCCTACAGTGAGTTTGCCTGTGCCCTGCTCTACTTCACCGGCTCTGCCCACTTCAACCGGTCCA
  TGAGAGCTCTGGCCAAGACCAAGGGCATGAGCCTGTCAGAGCATGCCCTTAGTGCTGCTGTGGTCCGGAA
  CAGCCAAGGTGTCAAGGTCGGGGCTGGACAAGTGCTGCCCACCCCCACAGAGAAGGACGTCTTCAAGCTC
  TTAGGCCTGCCCTACCGAGAACCGGCTGAACGGGACTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211260 representing NM_001014168
  Red=Cloning site Green=Tags(s)
  
  MDPQGILKAFPKRKKIHADPSSNALAKIPKREAGDARGWLSSLRAHIMPTGIGRARAELFEKQIIQHGGQ
  VCSAQAPGVTHIVVDEGMDYERALRLLRLPQLPPGAQLVKSAWLSLCLQEKKLTDTDGFSLSSPKRSLNE
  PQPSKSGQDASAPGTQGVLPRTTLSLSPPCTRAVSPPPKAEKPPKTQTQLSSEDEASDGEGPQVSSADLQ
  ALISGHYPTPPGEDGGPDPAPEALGKWVCAQPSSQKATNYNLHITEKLEVLAKAYNVQGDKWRALGYAKA
  INALKSFHKPVSSYQEACSIPGVGRRMAEKVMEILESGHLRKLDHISDSVPVLELFSNIWGAGTKTAQMW
  YHQGFRSLEDIRGLASLTAQQAIGLKHYDDFLDRMPREEAAEIEQMVRVSAQAFNPGLLCVACGSFRRGK
  VTCGDVDVLITHPDGRSHQGIFSPLLDSLRQQGFLTDDLVSQEENGQQQKYLGVCRLPGAGQRHRRLDII
  VVPYSEFACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSAAVVRNSQGVKVGAGQVLPTPTEKDVFKL
  LGLPYREPAERDW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014168
• ORF Size: 1719 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014168.1, NP_001014190.1
• RefSeq Size: 2320 bp
• RefSeq ORF: 1722 bp
• Locus ID: 361767
• Cytogenetics: 1q54
• MW: 62.4 kDa