Dock8 (NM_001037793) Rat Tagged ORF Clone

CAT#: RR211319

Dock8 (Myc-DDK-tagged ORF) - Rat dedicator of cytokinesis 8 (Dock8), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dock8
• Synonyms: MGC116414
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211319 representing NM_001037793
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACGCTACCGAGCGCCGAGCGCCGCGCCTTCGCGCTCAAGATCAACAGGTATTCATCATCAGAAA
  TAAGGAAGCAGTTTACGCTCCCGCCCAACCTCGGACAGTACCATCGGCACAGCCTCAGTACCTCTGGTTT
  CCCTTCTCTTCAGCTACCTCAGCTGTATGAGCCCGTTGAGCCTGTGGACTTTGAAGGACTTGTGATGACG
  CACTTAAACAGCTTGGATGCAGAGCTGGCCCAGGAGCTGGGGGACCTCACCGATGACGACCTGCATGTGG
  CCTTCACACCCAAGGAGTGTAGGACTTTGCAGCACTCTCTGCCAGAGGAAGGAGTTGAACTGGATCCTCA
  CGTCAGGGACTGTGTTCAGACCTATATTCGAGAGTGGCTGATTGTGAACCGGAAAAACCAAGGAAGTCCA
  GAGTTTTGCAGCTTCAGAAAGACTGGATCTCGCAGAGATTTTCACAAGACGCTTCAGAAACAGACGTTTG
  AGTCAGAAACCTTGGAGTGCAGTGAACCGGCCACTCAGACGGGACCCCGCCATCGCTTCAACGTGCTGTG
  TGATGTGTCTGGGAAGGGCCCCATCACTTCTTGTGACTTCGACCTCCGCAGCCTGCAGCCTGACGAGCGG
  CTGGAAAACCTGCTTCAGCTGAGTGCTGAGGACTTTGAGAAGGAGAAAGAGGAGGCCCGAAAGACCAATC
  GGACGGCTGAGCTCTTCGCCCTCTATCCATCCGTAGATGAGGAGGATGCTGTGGAAATCCGTCCAGTACC
  AGAATGTCCTAAGGAACATCTGGGCAACAGAATATTGGTGAAGGTGCTGACCCTGAAGTTTGAGATTGAA
  ATTGAACCTCTGTTTGCCAGTATTGCCCTCTATGATGTTAAAGAAAGGAAAAAGATCTCAGAAAACTTCC
  ACTGTGACCTGAACTCCGACCAGTTCAAAGGGTTTCTGCGAGCTCACACACCCTCGGTTGACCCGTCGAG
  TCAGGCAAGGTCTGCAGTGTTCTCGGTCACCTACCCATCTTCAGACATCTACCTGGTTGTCAAGATTGAA
  AAGGTCCTTCAGCAAGGAGAGATCGCAGACTGTGCAGAACCCTACATGATCATCAAAGAAAGCGATGGTG
  GAAAGAGTAAAGAAAAGGTTGAAAAACTAAAACTCCAAGCTGAATCCTTCTGCCAACGTTTGGGGAAATA
  CCGGATGCCCTTCGCCTGGGCCCCCATCAGCTTAGCAAGCTTCTTCAACGTCTCCACCCTTGAAAGGGAG
  AGCACAGACTTGGAGCCTGGGGCTGGGAGAAACTCTGTGGGGGAGAGGAGGAGTTTGTCCCAGTCCAGGA
  GGCCTTCTGAGAGAACCCTCTCCTTTGAGGAAAATGGAGTTGGATCCAACTTCAAAACCACCACCTTGGC
  CACTAACATCTTCTTCAAGCAGGAGGGAGATCGCCTTAGTGATGAAGACTTGTTCAAGTTTTTAGCTGAC
  TACAAGAGATCTTCGTCCCTACAGCGAAGAGTGAAATCCATCCCAGGCTCACTTAGGCTGGAGATAGCCC
  CAGCTCCTGACGTGATGAACTGCTGCCTGACACCCGAGATGCTGCCGGTCAAACCCTTCCCTGAAAATCG
  GACGCGTCCCCACAAGGAGATTTTGGAATTTCCAATCCGGGAAGTGTATGTTCCCCACACCGTGTACAGA
  AACCTTCTGTACGTATACCCACAGAGACTGAACTTTGCTAACAAGCTAGCATCCGCCCGGAACATTACAA
  TAAAGATTCAGTTTATGTGTGGAGAAGACCCCAGCAATGCTATGCCTGTCATCTTTGGGAAGTCCAGTGG
  GCCTGAATTTCTGCAGGAAGTGTACACAGCTATTACATACCATAATAAGTCTCCCGACTTTTATGAAGAA
  GTAAAGATTAAGCTTCCGGCCAAGCTCACAGTGAATCACCACCTCCTCTTCACCTTCTACCACATCAGCT
  GTCAGCAGAAGCAAGGCGCCTCTGGAGAAAGCCTTCTGGGATACTCGTGGCTGCCGATTCTCCTGAACGA
  ACGTCTTCAAACCGGATCCTACTGTCTACCAGTTGCCTTGGAAAAACTACCACCTAACTACTCCATACAT
  TCTGCTGAGAAAGTCCCTTTACAGAATCCTCCCATTAAGTGGGCCGAGGGCCATAAGGGGGTATTTAATA
  TTGAAGTGCAAGCTGTTTCCTCCGTTCACACCCAGGATAACCACCTGGAGAAGTTCTTCACCCTTTGCCA
  CTCCCTGGAGAGCCAGGTGACCTTCCCTATCCGCGTGCTGGACCAGAAGATCACGGAGAACACGCTGGAG
  CATGAGCTTAAGCTCAGCATCATCTGCCTCAACTCCTCCCGCCTGGAGCCCCTCGTGCTCTTCCTCCACC
  TGGTGCTGGATAAGCTCTTCCAGCTCTCTGTGCAGCCCATGGTCATCGCTGGCCAAACAGCAAACTTCTC
  CCAGTTTGCCTTCGAATCTGTGGTGGCCATTGCCAATAGCCTTCACAACAGCAAGGACCTGAGGAAGGAC
  CAGCACGGGAGGAACTGCCTGCTGGCCTCCTATGTGCACTACGTGTTCCGGCTGCCCGAGCTGCACAGGG
  ATGCACCCAAGTCAGGTGGCCCCACCGCCGTAGTCCCTGACCCCCGATACCACACATATGGACGCACGTC
  TGCCGCTGCAGTGAGTTCAAAGCTGATGCAGGCCCGTGTGATGAGCAGCAGTAACCCAGACCTGGCTGGG
  ACACACTGTGCAGCAGATGAGGAGGTTAAGAACATCATGTCTTCCAAGATTGCTGATCGCAACTGCAGCC
  GGATGTCTTACTATTGCTCTGGCAACAGTGACGTTCCAGGTTCAACTGCAGCCCCAAGGCCAGTCAGCAA
  AAAGCATTTCCATGAGGAGCTTGCCCTGCAGATGGTGGTCAGCACTGGACTAGTGAGAGAAACCGTCTTC
  AAGTACGCCTGGTTCTTCTTTGAGCTTCTGGTGAAAAGCATGGCACAGTACGTCCATAACCTGGATAAAC
  GGGACAGTTTTCGGAGGACTCGTTTTTCCGACCGCTTCAAGGATGACATAACTACCATTGTTAATGTGGT
  CACCTCGGAGATAGCAGCCCTTCTAGTGAAACCTCAGAAGGAAAGTGAGCAGGCCGAGAAGATCAACATC
  AGCCTGGCCTTCTTCCTGTATGACCTCCTCTCAATCATGGACAGAGGCTTCGTGTTCAACCTCATCAAGC
  ATTACTGCACTCAGCTATCAGCCAAGCTTAACACCCTTCCAACCCTCATTTCCATGCGGCTGGAGTTCCT
  GAGAATCCTCTGCAGCCACGAGCACTACCTCAACTTGAACCTGCTCTTCATGAATCCTGACACCGCACCA
  GCCTCTCCCTGTCCCTCCATATCCTCCCAGAACTCAAGCTCCTGCTCCAGTTTCCAGGACCAAAAGATTG
  CCAGCATGTTCGATCTGACCCCAGAGTACCGCCAGCAGCACTTCCTCACAGGGCTCCTCTTCACAGAACT
  GGCCGTTGCCCTGGATGCTGAAGGGGACGGAATTAGCAGAGTACAGAGAAAAGCCGTGAGTGCCATCCAC
  AGCCTTCTGAGTTCTCACGACCTGGATCCACGGTGTCTCAAACCAGAAGTGAAAGTCAAAATCGCTGCCC
  TTTACCTGCCGTTGGTCGGCATCATTCTGGATGCGCTGCCACAGCTCTATGACTTTACAGATGCTCGCAG
  TGGAAGGAGCCGTGCCAGTGGCTCATATGAAGAACAAGATGTGGCCAATGGAATCAACCAGAATGTGGCC
  CTGGCCATAGCAGGGAATCATTTTAATTTGAAGACCAGCGGAGCAATGCTGTCTTCCTTGCCCTATAAGC
  AGTACAACATGCTGAACGCGGACACTACCCGCCACCTCATGGTCTGCTTTCTGTGGATCATGAAAAACGC
  GGATCAGAGCCTCATCAGGAAGTGGATCGCCGACCTGCCTTCCATGCAGCTCAACAGGATTCTAGACCTG
  CTGTTCATCTGTGTCTCCTGCTTTGAATACAAGGGAAAGCAGAGTTCTGACAAAGTCAGTAACCAGGTCC
  TGCAGAAGTCAAGAGATGTCAAGGCCAAGTTGGAAGAAGCCCTGCTCCGTGGGGAAGGAGCCCGGGGGGA
  GATGATGCGACGTCGGATTCCAGGCACTGACCGGTTTCCAGGCCTAAATGAGAATCTGAGGTGGAGGAAG
  GAGCAGACACAGTGGCGGCAAGCTAACGAGAAGCTGGACAAAACAAAGGCAGAGTTAGATCAAGAAGCCT
  TGATCAGTGGCAACCTGGCTACAGAGGCTAATTTGATCATACTGGACATGCAGGAAAACATCATCCAGGC
  AAGCTCCTCCCTGGACTGTAAAGACAGCCTGCTGGGAGGTGTCCTCCGGGTCCTGGTGAATTCTCTGAGC
  TGTGATCAGAGCACCACCTACCTGACTCACTGTTTTGCAACTCTCCGAGCCCTCATCGCCAAGTTTGGAG
  ACCTGCTGTTCGAGGAGGAAATGGAGCAGTGTGCTGACCTGTGTCAGCGGGTGCTCCATCACTGCAGCAG
  TAGCATGGATGTCACCCGGAGCCAAGCCTGCGCCACCCTCTATCTACTCATGCGGTTCAGCTTCGGAGCC
  ACCAGTAACTTTGCAAGAGTAAAGATGCAGGTAACCATGGCACTGGCATCCTTGGTAGGCAAAGCACCAG
  ACTTCAATGAGGAGCACCTGAGAAAGTCCTTAAGGACCATTCTGGCCTATTCAGAAGAGGACACGGCCAT
  GCGGGCAACTCTTTTTCCCACGCAGGTGGAGGAGCTTCTCTGCAATCTCAACAGCATTTTATACGACACA
  GTGAAGATGAGGGAATTCCAGGAGGACCCTGAGATGCTCATGGACCTCATGTACAGAATTGCCAAGAGCT
  ACCAGGCATCCCCTGACCTGCGGCTGACCTGGCTCCAGAACATGGCAGAGAAACACACTAAGAGGAAGTG
  CTTCACAGAGGCCGCCATGTGCCTGGTGCATGCAGCAGCCTTGGTGGCAGAGTACCTGAGCATGCTGGAG
  GACCACAGTTACCTGCCTGTGGGCAGCGTCAGCTTTCAGAATATTTCTTCCAATGTGCTTGAGGAATCTG
  CAGTCTCCGATGACACCTTGTCACCTGATGAGGACGGTGTATGCTCTGGTCGCTACTTCACTGAGAGTGG
  CCTGGTGGGCCTCCTGGAGCAGGCTGCAGAGCTCTTCAGCACGGGAGGCTTGTACGAGACAGTTAATGAG
  GTCTACAAGCTGGTCATTCCCATCCTGGAAGCACATAGAGATTTCCGGAAGCTGACCTCTACTCACGACA
  AGCTGCAGAAGGCCTTTGACAACATCATCAACAAGGACCATAAGAGGATGTTTGGAACTTACTTCCGAGT
  TGGTTTCTACGGATCCAAATTTGGGGATTTGGATGAGCAGGAATTTGTGTACAAGGAACCTGCAATTACA
  AAGCTCCCCGAGATCTCACATAGACTAGAGGGATTTTATGGCCAGTGTTTTGGTGCCGAGTTTGTGGAAG
  TGATAAAGGACTCTACTCCCGTGGATAAAACCAAGTTGGATCCTAACAAGGCCTACATACAGATCACTTT
  TGTGGAGCCTTACTTTGATGAGTATGAGATGAAAGACAGGGTGACCTACTTCGAGAAGAATTTCAACCTT
  CGGAGGTTCATGTACACCACCCCCTTCACCCTGGAGGGGAGGCCCCGGGGAGAGCTGCACGAGCAGCATC
  GCAGGAACACGGTGCTCACCACCATGCATGCCTTCCCCTACATCAAGACCAGGATCCGAGTCAGCCAGAA
  AGAGGAGTTCGTCTTGACTCCGATTGAAGTTGCCATTGAAGACATGAAGAAGAAGACCTTGCAGTTAGCG
  GTGGCCACTCACCAGGAGCCCCCTGACGCGAAGATGCTCCAGATGGTACTGCAAGGCTCTGTGGGAGCCA
  CCGTGAATCAGGGCCCACTGGAGGTGGCCCAAGTGTTCTTGGCTGAAATTCCGGCTGACCCAAAACTCTA
  CCGGCATCACAACAAGCTGAGGTTATGCTTCAAGGAGTTCATAATGCGATGCGGTGAGGCCGTAGAGAAG
  AACAGGAGACTCATTACCGCAGAGCAGCGGGAGTACCAGCAGGAACTCAAGAAGAACTACAACAGGCTGA
  AGGACAGCCTCAGGCCCATGATTGAGCGGAAAATCCCAGAGCTCTACAAGCCCATATTCAGGGTTGACAG
  TCAAAAGAGGGACTCTTTCCACAGATCTAGTTTCAGGAAATGTGAAACCCAGTTGTCACAGGGCAGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211319 representing NM_001037793
  Red=Cloning site Green=Tags(s)
  
  MATLPSAERRAFALKINRYSSSEIRKQFTLPPNLGQYHRHSLSTSGFPSLQLPQLYEPVEPVDFEGLVMT
  HLNSLDAELAQELGDLTDDDLHVAFTPKECRTLQHSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQGSP
  EFCSFRKTGSRRDFHKTLQKQTFESETLECSEPATQTGPRHRFNVLCDVSGKGPITSCDFDLRSLQPDER
  LENLLQLSAEDFEKEKEEARKTNRTAELFALYPSVDEEDAVEIRPVPECPKEHLGNRILVKVLTLKFEIE
  IEPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSVDPSSQARSAVFSVTYPSSDIYLVVKIE
  KVLQQGEIADCAEPYMIIKESDGGKSKEKVEKLKLQAESFCQRLGKYRMPFAWAPISLASFFNVSTLERE
  STDLEPGAGRNSVGERRSLSQSRRPSERTLSFEENGVGSNFKTTTLATNIFFKQEGDRLSDEDLFKFLAD
  YKRSSSLQRRVKSIPGSLRLEIAPAPDVMNCCLTPEMLPVKPFPENRTRPHKEILEFPIREVYVPHTVYR
  NLLYVYPQRLNFANKLASARNITIKIQFMCGEDPSNAMPVIFGKSSGPEFLQEVYTAITYHNKSPDFYEE
  VKIKLPAKLTVNHHLLFTFYHISCQQKQGASGESLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYSIH
  SAEKVPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLCHSLESQVTFPIRVLDQKITENTLE
  HELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQTANFSQFAFESVVAIANSLHNSKDLRKD
  QHGRNCLLASYVHYVFRLPELHRDAPKSGGPTAVVPDPRYHTYGRTSAAAVSSKLMQARVMSSSNPDLAG
  THCAADEEVKNIMSSKIADRNCSRMSYYCSGNSDVPGSTAAPRPVSKKHFHEELALQMVVSTGLVRETVF
  KYAWFFFELLVKSMAQYVHNLDKRDSFRRTRFSDRFKDDITTIVNVVTSEIAALLVKPQKESEQAEKINI
  SLAFFLYDLLSIMDRGFVFNLIKHYCTQLSAKLNTLPTLISMRLEFLRILCSHEHYLNLNLLFMNPDTAP
  ASPCPSISSQNSSSCSSFQDQKIASMFDLTPEYRQQHFLTGLLFTELAVALDAEGDGISRVQRKAVSAIH
  SLLSSHDLDPRCLKPEVKVKIAALYLPLVGIILDALPQLYDFTDARSGRSRASGSYEEQDVANGINQNVA
  LAIAGNHFNLKTSGAMLSSLPYKQYNMLNADTTRHLMVCFLWIMKNADQSLIRKWIADLPSMQLNRILDL
  LFICVSCFEYKGKQSSDKVSNQVLQKSRDVKAKLEEALLRGEGARGEMMRRRIPGTDRFPGLNENLRWRK
  EQTQWRQANEKLDKTKAELDQEALISGNLATEANLIILDMQENIIQASSSLDCKDSLLGGVLRVLVNSLS
  CDQSTTYLTHCFATLRALIAKFGDLLFEEEMEQCADLCQRVLHHCSSSMDVTRSQACATLYLLMRFSFGA
  TSNFARVKMQVTMALASLVGKAPDFNEEHLRKSLRTILAYSEEDTAMRATLFPTQVEELLCNLNSILYDT
  VKMREFQEDPEMLMDLMYRIAKSYQASPDLRLTWLQNMAEKHTKRKCFTEAAMCLVHAAALVAEYLSMLE
  DHSYLPVGSVSFQNISSNVLEESAVSDDTLSPDEDGVCSGRYFTESGLVGLLEQAAELFSTGGLYETVNE
  VYKLVIPILEAHRDFRKLTSTHDKLQKAFDNIINKDHKRMFGTYFRVGFYGSKFGDLDEQEFVYKEPAIT
  KLPEISHRLEGFYGQCFGAEFVEVIKDSTPVDKTKLDPNKAYIQITFVEPYFDEYEMKDRVTYFEKNFNL
  RRFMYTTPFTLEGRPRGELHEQHRRNTVLTTMHAFPYIKTRIRVSQKEEFVLTPIEVAIEDMKKKTLQLA
  VATHQEPPDAKMLQMVLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAVEK
  NRRLITAEQREYQQELKKNYNRLKDSLRPMIERKIPELYKPIFRVDSQKRDSFHRSSFRKCETQLSQGS
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037793
• ORF Size: 6297 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037793.2, NP_001032882.2
• RefSeq Size: 7495 bp
• RefSeq ORF: 6300 bp
• Locus ID: 499337
• Cytogenetics: 1q51
• MW: 238.9 kDa