Dgat1 (NM_053437) Rat Tagged ORF Clone

CAT#: RR211358

Dgat1 (Myc-DDK-tagged ORF) - Rat diacylglycerol O-acyltransferase homolog 1 (mouse) (Dgat1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dgat1
• Synonyms: ARAT; Dgat
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211358 representing NM_053437
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGACCGCGGAGGCGCGGGAAGCTCTCGGCGTCGCAGGACCGGCTCGCGGGTTTCCGTCCAGGGAG
  GTAGTGGGCCCAAGGTAGAAGAGGACGAGGTGCGAGAAGCGGCTGTGAGCCCCGACTTGGGCGCCGGGGG
  TGACGCGCCGGCTCCGGCTCCGGCTCCAGCCCATACCCGGGACAAAGACCGGCAGACCAGCGTGGGCGAC
  GGCCACTGGGAGCTGAGGTGCCATCGTCTGCAAGACTCTTTGTTCAGCTCAGACAGCGGTTTCAGCAATT
  ACCGTGGTATCCTGAATTGGTGCGTGGTGATGCTGATCCTGAGTAATGCAAGGTTATCTTTAGAGAATCT
  TATCAAGTATGGCATCCTGGTGGATCCCATCCAGGTGGTGTCTCTGTTTCTGAAGGACCCCTACAGCTGG
  CCTGCCCCATGCTTGATCATTGCATCCAATATCTTTATTGTGGCTACATTTCAGATTGAGAAGCGCCTGT
  CAGTGGGTGCCCTGACAGAGCAGATGGGGCTGCTGCTACATGTGGTTAACCTGGCCACAATTATCTGCTT
  CCCAGCAGCTGTGGCCTTACTGGTTGAGTCTATCACTCCAGTGGGTTCCCTGTTTGCTCTGGCATCATAC
  TCCATCATCTTCCTCAAGCTTTCTTCCTACCGGGATGTCAATCTGTGGTGCCGCCAGCGAAGGGTCAAGG
  CCAAAGCTGTGTCTGCAGGGAAGAAGGTCAGTGGGGCTGCTGCCCAGAACACTGTAAGCTATCCGGACAA
  CCTGACCTACCGAGATCTCTATTACTTCATCTTTGCTCCTACTTTGTGTTATGAACTCAACTTTCCTCGA
  TCCCCCCGAATACGAAAGCGCTTTCTGCTACGGCGGGTTCTTGAGATGCTCTTTTTCACCCAGCTTCAAG
  TGGGGCTGATCCAGCAGTGGATGGTCCCTACTATCCAGAACTCCATGAAGCCCTTCAAGGACATGGACTA
  TTCACGAATCATTGAGCGTCTCTTAAAGCTGGCGGTCCCCAACCATCTGATATGGCTCATCTTCTTCTAT
  TGGCTTTTCCACTCATGTCTCAATGCTGTGGCAGAGCTCCTGCAGTTTGGAGACCGCGAGTTCTACAGGG
  ACTGGTGGAATGCTGAGTCTGTCACCTACTTTTGGCAGAACTGGAATATCCCCGTGCACAAGTGGTGCAT
  CAGACACTTTTACAAGCCTATGCTCAGACTGGGCAGCAACAAATGGATGGCCAGGACTGGGGTCTTTTGG
  GCGTCAGCTTTCTTTCATGAGTACCTAGTGAGCATTCCCCTGAGGATGTTCCGCCTTTGGGCATTCACAG
  CAATGATGGCTCAGGTCCCACTGGCCTGGATTGTGAACCGCTTCTTCCAAGGGAACTATGGCAATGCAGC
  TGTGTGGGTGACACTCATCATTGGGCAACCGGTGGCTGTGCTCATGTATGTCCACGACTACTACGTGCTC
  AACTATGATGCCCCAGTGGGGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211358 representing NM_053437
  Red=Cloning site Green=Tags(s)
  
  MGDRGGAGSSRRRRTGSRVSVQGGSGPKVEEDEVREAAVSPDLGAGGDAPAPAPAPAHTRDKDRQTSVGD
  GHWELRCHRLQDSLFSSDSGFSNYRGILNWCVVMLILSNARLSLENLIKYGILVDPIQVVSLFLKDPYSW
  PAPCLIIASNIFIVATFQIEKRLSVGALTEQMGLLLHVVNLATIICFPAAVALLVESITPVGSLFALASY
  SIIFLKLSSYRDVNLWCRQRRVKAKAVSAGKKVSGAAAQNTVSYPDNLTYRDLYYFIFAPTLCYELNFPR
  SPRIRKRFLLRRVLEMLFFTQLQVGLIQQWMVPTIQNSMKPFKDMDYSRIIERLLKLAVPNHLIWLIFFY
  WLFHSCLNAVAELLQFGDREFYRDWWNAESVTYFWQNWNIPVHKWCIRHFYKPMLRLGSNKWMARTGVFW
  ASAFFHEYLVSIPLRMFRLWAFTAMMAQVPLAWIVNRFFQGNYGNAAVWVTLIIGQPVAVLMYVHDYYVL
  NYDAPVGA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053437
• ORF Size: 1494 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053437.1, NP_445889.1
• RefSeq Size: 1751
• RefSeq ORF: 1497
• Locus ID: 84497
• MW: 56.9 kDa
• Gene Summary: enzyme that catalyzes the final step of triglyceride synthesis; uses diacylglycerol and fatty acyl CoA as substrates [RGD, Feb 2006]