Mdh2 (NM_031151) Rat Tagged ORF Clone

CAT#: RR211474

Mdh2 (Myc-DDK-tagged ORF) - Rat malate dehydrogenase 2, NAD (mitochondrial) (Mdh2), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mdh2
• Synonyms: Mor1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211474 representing NM_031151
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTCCGCTCTCGCCCGTCCTGTCGGTGCCGCTCTCCGCCGCAGCTTCAGCACTTCAGCCCAGAACA
  ATGCTAAAGTAGCTGTCCTGGGAGCTTCTGGGGGCATTGGGCAACCCCTTTCACTCCTCCTGAAGAACAG
  TCCCCTCGTGAGCCGCCTGACCCTCTATGACATCGCTCACACACCTGGTGTGGCAGCAGATCTGAGTCAC
  ATCGAGACCAGAGCAAATGTGAAAGGCTACCTCGGGCCGGAGCAGCTGCCGGACTGCCTAAAAGGTTGTG
  ATGTGGTGGTCATCCCAGCTGGAGTGCCCAGGAAGCCAGGAATGACACGAGATGACCTGTTCAACACCAA
  TGCTACCATTGTGGCCACATTGACGGCTGCCTGTGCCCAGCACTGTCCTGAAGCCATGATTTGCATCATT
  TCCAACCCAGTTAACTCCACCATCCCCATCACAGCCGAAGTTTTCAAGAAGCATGGCGTATACAACCCCA
  ACAAGATATTCGGTGTGACAACCCTTGACATCGTCAGAGCAAACACATTTGTGGCAGAGCTAAAGGGTTT
  GGACCCAGCTCGAGTCAATGTGCCTGTCATTGGTGGCCACGCCGGGAAGACGATCATCCCCCTGATCTCT
  CAGTGTACCCCCAAGGTTGACTTTCCCCAAGACCAGCTGGCCACACTCACCGGGAGGATCCAGGAGGCTG
  GCACTGAAGTCGTGAAGGCCAAGGCTGGAGCAGGCTCTGCCACTCTGTCCATGGCTTATGCTGGAGCCCG
  CTTTGTCTTCTCCCTGGTGGACGCCATGAATGGGAAGGAAGGAGTCATCGAGTGCTCTTTTGTTCAGTCC
  AAAGAGACAGAGTGCACTTATTTCTCTACACCCTTGCTGTTGGGGAAAAAAGGCCTGGAGAAGAACCTAG
  GCATTGGCAAAATCACTCCTTTTGAGGAGAAAATGATTGCCGAGGCCATCCCTGAGCTGAAAGCCTCCAT
  CAAGAAAGGCGAGGACTTTGTGAAGAACATGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211474 representing NM_031151
  Red=Cloning site Green=Tags(s)
  
  MLSALARPVGAALRRSFSTSAQNNAKVAVLGASGGIGQPLSLLLKNSPLVSRLTLYDIAHTPGVAADLSH
  IETRANVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICII
  SNPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLIS
  QCTPKVDFPQDQLATLTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVIECSFVQS
  KETECTYFSTPLLLGKKGLEKNLGIGKITPFEEKMIAEAIPELKASIKKGEDFVKNMK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031151
• ORF Size: 1014 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031151.1, NM_031151.2, NP_112413.2
• RefSeq Size: 1324
• RefSeq ORF: 1017
• Locus ID: 81829
• MW: 35.7 kDa
• Gene Summary: catalyzes the conversion of pyruvate or oxaloacetate to malate [RGD, Feb 2006]