Tia1 (NM_001012096) Rat Tagged ORF Clone

CAT#: RR211505

Tia1 (Myc-DDK-tagged ORF) - Rat TIA1 cytotoxic granule-associated RNA binding protein (Tia1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tia1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211505 representing NM_001012096
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGACGAGATGCCCAAGACTCTATACGTCGGTAACCTTTCTCGAGATGTGACAGAAGCTCTCATCC
  TCCAGCTCTTTAGCCAGATCGGACCTTGTAAAAACTGCAAAATGATTATGGATACGGCAGGAAATGACCC
  GTACTGTTTCGTGGAGTTCCATGAGCATCGCCACGCAGCTGCAGCCCTGGCTGCTATGAATGGGCGGAAG
  ATAATGGGTAAGGAAGTGAAAGTGAATTGGGCAACAACCCCTAGCAGTCAAAAGAAAGATACAAGCAATC
  ATTTCCATGTGTTTGTTGGTGACCTCAGTCCAGAAATCACAACTGAAGACATCAAAGCAGCTTTTGCACC
  ATTTGGAAGAATTTCAGATGCCCGTGTGGTAAAAGACATGGCTACCGGGAAGTCTAAGGGATATGGCTTT
  GTCTCCTTTTTCAACAAATGGGATGCAGAAAATGCCATTCAGCAGATGGGTGGCCAGTGGCTTGGTGGAA
  GACAAATCAGAACTAACTGGGCAACCCGAAAGCCTCCAGCTCCAAAGAGTACATATGAGTCAAACACCAA
  ACAGCTGTCATACGATGAGGTTGTGAGTCAGTCTAGTCCCGGCAACTGTACCGTGTACTGTGGAGGAGTG
  ACGTCAGGACTGACAGAACAACTAATGCGTCAGACTTTTTCTCCATTTGGACAAATAATGGAAATCCGAG
  TCTTTCCAGATAAAGGGTACTCATTCATTCGGTTCAGTTCCCATGAAAGTGCGGCACATGCAATTGTTTC
  TGTTAACGGTACTACCATTGAAGGGCATGTGGTGAAATGTTACTGGGGCAAAGAGACTCTTGACATGATA
  AACCCCGTGCAGCAGCAAAATCAAATTGGATATCCACCAGCATATGGCCAGTGGGGCCAGTGGTATGGAA
  ATGCACAACAGATTGGCCAGTATGTACCTAATGGTTGGCAAGTACCTGCCTATGGAATGTATGGCCAGCC
  ATGGAGCCAGCAGGGATTCAATCAGACCCAGTCTTCTGCACCATGGATGGGACCCAATTACAGTGTGCCG
  CCACCTCAAGGGCAGAACGGCGGCATGCTGCCTAATCAGCCTGCTGGGTATCGAGTGGCAGGGTATGAAA
  CCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211505 representing NM_001012096
  Red=Cloning site Green=Tags(s)
  
  MEDEMPKTLYVGNLSRDVTEALILQLFSQIGPCKNCKMIMDTAGNDPYCFVEFHEHRHAAAALAAMNGRK
  IMGKEVKVNWATTPSSQKKDTSNHFHVFVGDLSPEITTEDIKAAFAPFGRISDARVVKDMATGKSKGYGF
  VSFFNKWDAENAIQQMGGQWLGGRQIRTNWATRKPPAPKSTYESNTKQLSYDEVVSQSSPGNCTVYCGGV
  TSGLTEQLMRQTFSPFGQIMEIRVFPDKGYSFIRFSSHESAAHAIVSVNGTTIEGHVVKCYWGKETLDMI
  NPVQQQNQIGYPPAYGQWGQWYGNAQQIGQYVPNGWQVPAYGMYGQPWSQQGFNQTQSSAPWMGPNYSVP
  PPQGQNGGMLPNQPAGYRVAGYETQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012096
• ORF Size: 1125 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012096.1, NP_001012096.1
• RefSeq Size: 1435 bp
• RefSeq ORF: 1128 bp
• Locus ID: 312510
• Cytogenetics: 4q34
• MW: 41.6 kDa