Slc30a3 (NM_001013243) Rat Tagged ORF Clone

CAT#: RR211546

Slc30a3 (Myc-DDK-tagged ORF) - Rat solute carrier family 30 (zinc transporter), member 3 (Slc30a3), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc30a3
• Synonyms: ZnT3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211546 representing NM_001013243
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCTTCTCCGGCCAGCGGAGGCTCGGAGACCACCCGCCTAGTGAGTCCCCGAGACCGCAGCAGCG
  CCGGCGGCGGCCTGCGATTGAAGAGTCTTTTCACAGAGCCCTCAGAGCCCCTGCCGGAGGGGCCCAAGCT
  TGAGGGGATGGCCTTCCACCACTGCCACAAGAACCGCGTGTCTCAGTCAGGCCTCTCTCCAGAGAGGGCG
  CAGGCCCGGAGGCAGCTGTATGCTGCCTGCGTCGTGTGCTTCATCTTCATGGCTGGGGAGGTGGTTGGTG
  GGTATTTAGCACACAGCTTGGCCATCATGACCGACGCCGCCCACTTGCTGGCAGACATAGGCAGTATGAT
  GGCCAGCCTCTTTTCCCTCTGGCTCTCTACTCGGCCAGCCACCCGCACCATGACCTTTGGCTGGCACCGC
  TCAGAGACTCTGGGAGCTTTGGCCTCCGTGGTCTCCCTCTGGATAGTCACCGGCATCCTCCTGTACCTGG
  CCTTCCTCCGCCTGCTTCACAGTGACTACCACATTGAGGCGGGCGCCATGCTGCTGACTGCCAGCATTGC
  TGTCTGTGCCAACATGATAATGGCCTTTGTGCTGCACCAGACTGGAGCCCCCCACAGCCACGGACCTAGG
  GGTGCAGAGTATGCACCACTGGAGGAGGGGCACGGGCACCCACTGTCCCTGGGAAACACCAGTGTCCGGG
  CTGCCTTTGTGCATGTGCTGGGAGATCTCCTTCAGAGCCTCGGGGTCCTGGCCGCCTCTATCCTCATCTA
  CTTCAAGCCCCAGTACAAGGTGGCCGACCCCATCAGCACCTTCCTCTTCTCTATCTGCGCCCTTGGGTCC
  ACAGCTCCTACACTTCGAGATGTTCTCCTCGTCCTCATGGAAGGTGCCCCTCGGAGTGTGGAATTTGAGC
  CTGTAAGGGACACCTTGTTGTCAGTGCCGGGTGTCCGAGCCACCCATGATCTCCACCTGTGGGCGCTGAC
  GCTTACTTACCACGTTGCCTCCGCACACCTGGCTATTGACTCCACGGCTGACCCTGAAGCCATCCTGGCT
  GAAGCCTCATCCCGGCTCTATTCCCGGTTTGGATTCTCTAGCTGCACCCTGCAGGTGGAGAAATACCGGT
  CTGAGATGGCCCACTGTCTTCGCTGCCGGGAGCCCCCCAAAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211546 representing NM_001013243
  Red=Cloning site Green=Tags(s)
  
  MEPSPASGGSETTRLVSPRDRSSAGGGLRLKSLFTEPSEPLPEGPKLEGMAFHHCHKNRVSQSGLSPERA
  QARRQLYAACVVCFIFMAGEVVGGYLAHSLAIMTDAAHLLADIGSMMASLFSLWLSTRPATRTMTFGWHR
  SETLGALASVVSLWIVTGILLYLAFLRLLHSDYHIEAGAMLLTASIAVCANMIMAFVLHQTGAPHSHGPR
  GAEYAPLEEGHGHPLSLGNTSVRAAFVHVLGDLLQSLGVLAASILIYFKPQYKVADPISTFLFSICALGS
  TAPTLRDVLLVLMEGAPRSVEFEPVRDTLLSVPGVRATHDLHLWALTLTYHVASAHLAIDSTADPEAILA
  EASSRLYSRFGFSSCTLQVEKYRSEMAHCLRCREPPKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013243
• ORF Size: 1164 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013243.1, NP_001013261.1
• RefSeq Size: 1690 bp
• RefSeq ORF: 1167 bp
• Locus ID: 366568
• Cytogenetics: 6q14
• MW: 41.9 kDa