Ndufa5 (NM_012985) Rat Tagged ORF Clone

CAT#: RR211640

Ndufa5 (Myc-DDK-tagged ORF) - Rat NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 5 (Ndufa5), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ndufa5
• Synonyms: NADHUO
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211640 representing NM_012985
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCTTGGTGAAGAAGACTACTGGCCTGGTGGGATTGGCCGTTTGCGACACTCCACACGAGAGGC
  TAACAATATTGTATACAAAGATTCTTGATCTTCTGAAGCACTTTCCTAAACATGCAGCCTACAGAAAGTA
  CACAGAACAGATCACCAGTGAGAAGCTGGAGTTGGTCAAATTGGAACCAGATGTTAAAAAATTAGAAAAC
  TTGCTTCAGGGTGGTGAAGTAGAAGAGGTGATTCTTCAGGCTGAAAAAGAACTAAGTCTGGCAAGAAAAA
  TGTTGCAGTGGAAGCCCTGGGAGCCACTGGTGGAGGAGCCCCCTGCTAACCAGTGGAAGTGGCCAATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211640 representing NM_012985
  Red=Cloning site Green=Tags(s)
  
  MAGLVKKTTGLVGLAVCDTPHERLTILYTKILDLLKHFPKHAAYRKYTEQITSEKLELVKLEPDVKKLEN
  LLQGGEVEEVILQAEKELSLARKMLQWKPWEPLVEEPPANQWKWPI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012985
• ORF Size: 348 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012985.1, NM_012985.2, NP_037117.1
• RefSeq Size: 552
• RefSeq ORF: 351
• Locus ID: 25488
• MW: 13.4 kDa
• Gene Summary: human homolog is a subunit of complex I of the respiratory chain which transfers electrons from NADH to ubiquinone [RGD, Feb 2006]