Spam1 (NM_053967) Rat Tagged ORF Clone

CAT#: RR211737

Spam1 (Myc-DDK-tagged ORF) - Rat sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (Spam1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Spam1
• Synonyms: Spam
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211737 representing NM_053967
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGAGTTGCAATTTAAGTGGCTCTTTTGGAGGAGCTTTGCTGAGTCTGGGGGCACATTCCAAACAG
  TGTTAATCTTTCTTTTCATTCCATATTCCTTGACTGTGGATTATAGGGCAACACCAGTTCTGTCAGATAC
  AACTTTCGTTTGGGTCTGGAATGTTCCAACTGAAGCTTGTGTAGAAAACGTTACTGAACCAATAGATCTG
  AGCTTCTTCTCTTTAATTGGAAGCCCCCGGAAAACTGCCATAGGGCAGCCTGTCACATTATTTTATGTTG
  ATCGACTTGGTAACTATCCTCACATAGATGCACAGCAAACAGAACATCATGGAGGAATACCTCAGAAGGG
  GGATTTGACAACTCATTTGGTGAAAGCTAAGGAAGACGTAGAGCGTTACATACCAACAGACAAACTGGGT
  TTAGCTATCATTGACTGGGAAGAATGGAGGCCTACCTGGATGAGAAACTGGACACCTAAGGATATTTACA
  GGAATAAGTCTATTGAATTGGTCCAGGCAGCTGATCCAGCAATTAATATCACAGAAGCTACTGTGAGAGC
  CAAAGCACAATTTGAAGGGGCAGCTAAAGAGTTTATGGAAGGGACTTTAAAATTGGGGAAACATATTCGA
  CCAAAGCACTTATGGGGTTTTTATCTATTTCCTGATTGTTATAACAATAAATTTCAAGTCGATAACTATG
  ATGGCCAATGCCCTGATGTGGAAAAGAAACGAAACGATGATCTTGACTGGCTATGGAAAGAAAGCACGGG
  CCTTTACCCATCTGTCTATTTGAAGAAAGATTTGAAGTCGAGTCGAAAAGCAACCCTTTATGTCCGCTAC
  CGAGTTTTAGAATCTATCAGAGTGTCCAAAGTTTCAGATGAATCGAATCCAGTTCCAATTTTTGTCTATA
  TCCGTCTTGTTTTCACTGATCATGTCTCTGAATACCTTCTAGAGGATGATCTTGTGAATACAATTGGTGA
  AATTGTTGCTCAGGGTACCTCTGGAATTATAATATGGGATGCTATGTCTTTAGCACAACGTTCGGCAGGT
  TGCCCAATCCTACGTCAATACATGAAGACAACCCTGAATCCATACATAGTCAATGTCACCCTAGCAGCCA
  AAATGTGCAGTCAAACACTTTGTAAGGAGAAAGGCATGTGTTCGAGAAAAACAGAAAGTTCAGATGCATA
  TCTTCACTTGGATCCAAGTAGTTTTTCTATTAATGTAACAGAAGCTGGAAAGTATGAAGTTCTTGGCAAG
  CCCGAGGTTAAAGACTTGGAATACTTTTCTGAACATTTTAAATGCAGCTGTTTTAGCAAAATGACATGTG
  AGGAGACATCTGATATGCGAAGTATACAAGATGTGAATGTGTGCATGGGTGACAATGTTTGTATAAAAGC
  CACGCTAGGACCCAACTCAGCATTCCACCTCCTACCTGGCAAAGGCCTCCTCCTTATGACAACACTTGCT
  CACATCTTGCACCATCTACCACATGATATTTTTGTTTTTCCGTGGAAGATGCTAGTCAGTACTCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211737 representing NM_053967
  Red=Cloning site Green=Tags(s)
  
  MGELQFKWLFWRSFAESGGTFQTVLIFLFIPYSLTVDYRATPVLSDTTFVWVWNVPTEACVENVTEPIDL
  SFFSLIGSPRKTAIGQPVTLFYVDRLGNYPHIDAQQTEHHGGIPQKGDLTTHLVKAKEDVERYIPTDKLG
  LAIIDWEEWRPTWMRNWTPKDIYRNKSIELVQAADPAINITEATVRAKAQFEGAAKEFMEGTLKLGKHIR
  PKHLWGFYLFPDCYNNKFQVDNYDGQCPDVEKKRNDDLDWLWKESTGLYPSVYLKKDLKSSRKATLYVRY
  RVLESIRVSKVSDESNPVPIFVYIRLVFTDHVSEYLLEDDLVNTIGEIVAQGTSGIIIWDAMSLAQRSAG
  CPILRQYMKTTLNPYIVNVTLAAKMCSQTLCKEKGMCSRKTESSDAYLHLDPSSFSINVTEAGKYEVLGK
  PEVKDLEYFSEHFKCSCFSKMTCEETSDMRSIQDVNVCMGDNVCIKATLGPNSAFHLLPGKGLLLMTTLA
  HILHHLPHDIFVFPWKMLVSTP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053967
• ORF Size: 1536 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053967.1, NM_053967.2, NP_446419.1
• RefSeq Size: 2112
• RefSeq ORF: 1539
• Locus ID: 117037
• MW: 58.4 kDa
• Gene Summary: a glycoprotein responsible for sperm adhesion to the zona pellucida [RGD, Feb 2006]