Mtmr3 (NM_001012038) Rat Tagged ORF Clone

CAT#: RR211921

Mtmr3 (Myc-DDK-tagged ORF) - Rat myotubularin related protein 3 (Mtmr3), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mtmr3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211921 representing NM_001012038
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCATAGCCTTGAGTGCATCCAGGCCAATCAGATCTTTCCCAGGAAGCAGCTGATTCGGGAGGATG
  AAAATCTTCAGGTTCCTTTCCTTGAACTCCATGGAGAGAGCACAGAGTATGTGGGCCGTGCTGAGGATGC
  CATCATTGCCCTCTCTAATTACAGACTTCACATCAAGTTTAAGGAGTCTCTTGTGAATGTTCCATTACAG
  CTTATAGAAAGCGTTGAATGCCGTGATATATTTCAGCTTCACTTGACATGCAAAGACTGCAAAGTTATCA
  GGTGTCAGTTCCCAACCTTTGAGCAGTGTCAAGATTGGCTTAAGAGATTAAACAATGCAATCCGACCACC
  TGGAAAAATAGAAGATCTCTTCTCATTTGCATACCATGCTTGGTGCATGGAGGTCTATGCCAGTGAAAAA
  GAGCAGCATGGAGACCTGTGCAGACCAGGGGAACATGTAACGTCAAGGTTTAAGAATGAAGTGGAGCGGA
  TGGGTTTTGATATGAACAATGCCTGGAGGATTTCCAACATCAATGAGAAATACAAATTATGTGGTAGTTA
  TCCACAAGAGCTCATAGTACCTGCCTGGATCACTGACAAAGAACTAGAAAGCGTGGCAGGCTTCAGATCC
  TGGAAGCGCATCCCCGCCGTCATCTACAGGCACCAGAGCAATGGAGCCGTCATTGCCCGCTGCGGACAGC
  CCGAGGTTAGCTGGTGGGGCTGGCGGAATGCTGATGATGAACATCTGGTGCAGTCAGTGGCCAAGGCTTG
  TGCTTCTGACTCTCAGTCAAGTGTTGGCAAGGTTTCCACTCGGAACAGTTGTCGGGGCTTTCCCAATGCA
  GGGGATCTTTCTGATGTGGAGTTCGATGCCTCTCTGTCAAATGCTTCAGGAACAGAAAGTTTAGCTCTCC
  AGCCACAGAAGCTTTTGATCTTGGACGCACGCTCCTATGCAGCAGCTGTGGCAAATCGAGCCAAAGGAGG
  GGGCTGTGAGTGTCCAGAGTATTATCCAAACTGTGAAGTTGTGTTTATGGGAATGGCAAACATACATTCT
  ATCCGGAGGAGTTTCCAGTCTCTGCGACTGCTGTGCACACAGATGCCAGATCCTGGAAATTGGCTTTCTG
  CTCTTGAAAGTACGAAGTGGCTCCACCACCTGTCTGTGCTTCTGAAGTCGGCACTTCTCGTGGTGCATGC
  TGTGGACCGAGATCAGCGACCAGTGCTGGTGCACTGCTCAGATGGCTGGGATCGCACTCCCCAAATCGTG
  GCCCTGGCTAAGCTTCTGCTGGACCCTTATTACCGAACCGTAGAGGGTTTCCAAGTCCTTGTGGAGATGG
  AATGGCTGGATTTTGGCCATAAGTTTGCTGATCGGTGTGGTCATGGGGAGAACTCAGATGACCTGAATGA
  GCGTTGCCCAGTGTTTCTCCAATGGCTTGACTGTGTTCATCAACTCCAGAGGCAATTTCCTTGCTCTTTT
  GAGTTCAACGAAGCATTCCTTGTGAAGCTGGTTCAGCACACCTATTCCTGTCTCTTTGGGACATTCCTGT
  GCAACAACGCCAAGGAGAGGGGGGAGAAGCAGACTCAGGAACGGACATGCTCGGTGTGGTCGCTTCTGCG
  GGCCGGCAACAAGGCCTTCAAAAACCTGCTATATTCATCCCAGTCAGAAGCCGTGCTGTACCCTGTGTGC
  CATGTGCGGAATCTGATGCTGTGGAGTGCAGTGTACCTTCCCTGCCCGTCCCCATCCACCCCCACAGACG
  ACAGCTGTGCACCATACCCAGCCCCAGGCACCAGCCCTGATGAGCCTCCACTGAGCCGGCTACCCAAGAC
  TAGATCATTTGACAATCTGACCACAACCTGTGACAACATGGTGCCGCTGGCCAGCCGGCGCAGCAGTGAC
  CCCAGCCTGAATGAGAAGTGGCAGGAGCACGGGCGCTCGCTGGAGTTGAGCAGCTTTGCTGGCTCTGGGG
  AAGAGGTGCCTGCCATAGACAGCCTGCGCAGGCCCAGCAGGCTGCTCGGAGGAGCCGAGCTATCTGTGGC
  AGCTGGTGTGGCTGAAGGGCAGATGGAGAATATTTTGCAAGAGGCCACCAAAGAGGAGAGTGGGGTAGAA
  GAGCCCACCCACAGGGAGCACACTGAGGTGCCAGAGGTCAAAGAGGAGGCACCCCTAGCAAAAGAGAGTA
  GGACGGCAGCTCAGGGCTCAGGTGTACTTTACCAAGAACCACAGCTGGATGATGCTACTCTAAGGAGCCA
  TTTAGGCCCCAGCCTGTCTTCGTTCTCCCAGGGTATTCCTGAGCATCGGGAGGTGGGGCACAGTGTTCTC
  TCTAGTTCACTCCCAGCTTCTCTCAGGGGAGAGGACTCCCAGGAGGTCCCTGTAGAACAGCCTCAAGTAG
  AGAACATTGCAGAGGACAGGGAGAATGTAGTCCCTGCTGTCCCAGTAGATGTAAAAATTGGCCTTGGTAC
  CTCAGAGTCTAGTCCTCTGCTGCCTTCCCAAGTCCCATTCGAGACAAGAGGACCACACATGAACAACTCC
  GTGCACATGTTATTAGAAGATAAGGTGAAGTCCGAGAGTGGGCCCCAGCTCCATCACAGACCCTGCCTGG
  CAAGCAGTGGTAGATTCAGTGGCAAGGACATGCTTCCTATAGCACCAGAGCCCAGGTCTGCTGAGAGGCC
  CCAGTGGGACTCTGTGCTACACAGGACTTCTTCCCCTGGCAACACCCTCAGCCTGATGATGCAGGCTCCT
  TGTGCCTTGCCATTAGACAAATGTAGACAGAGAATTGTGTGCAACGGTGCCCTAGAGACTGAAAACAAGG
  CCTCAGAACAGCCTGCAGGGTTTGACACCCTTCAGAAGTACCCCACACCCAATGGGCATTGTGCCAATGG
  GGAGACTGGGAGGAGTAAGGACTCACTGAGCCACCAGCTGTCTGCCACAAGCTATAGCTCTGCTCACTCG
  TGCTCAAGGAACTTGCACCACAAGTGGCTGAATAGCCACTCGGGGAGGCCATCCACTACCAACAGCCCTG
  AGCAGCCTTCCCGCAGCCACCTGGATGATGACGGCATGCCTGTGTACACAGACACGATCCAACAGCGCCT
  GCGACAGATAGAGTCTGGTCACCAGCAGGAAGTGGAGACCTTGAAGAAACAAGTCCAGGAACTGAAGAGT
  CGCCTGGAGAGCCAGTACCTGACCAGCTCTCTGCGCTTCAATGGGGACTTTGGAGATGAAGTGACTTCAA
  TCCCCGACTCGGAAAGCAATCTGGATCAGAACTGTTTGTCTCGCTGCAGCACAGAGATTTTCTCTGAAGC
  CAGCTGGGAGCAGGTGGATAAACAGGACACAGAGATGACCCGTTGGCTACCTGACCACTTGGCTGCCCAC
  TGCTATGCCTGTGACAGTGCCTTCTGGCTTGCCAGTAGGAAGCACCACTGCAGGAATTGTGGGAACGTAT
  TCTGCTCCAGTTGTTGTAACCAGAAGGTTCCAGTTCCCAGCCAGCAGCTCTTTGAACCCAGTCGAGTGTG
  CAAGTCTTGCTATAGCAGCCTCCATCCCACAAGCTCCAGCATTGACCTTGAACTGGATAAGCCTATTGCT
  GCCACTTCAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211921 representing NM_001012038
  Red=Cloning site Green=Tags(s)
  
  MRHSLECIQANQIFPRKQLIREDENLQVPFLELHGESTEYVGRAEDAIIALSNYRLHIKFKESLVNVPLQ
  LIESVECRDIFQLHLTCKDCKVIRCQFPTFEQCQDWLKRLNNAIRPPGKIEDLFSFAYHAWCMEVYASEK
  EQHGDLCRPGEHVTSRFKNEVERMGFDMNNAWRISNINEKYKLCGSYPQELIVPAWITDKELESVAGFRS
  WKRIPAVIYRHQSNGAVIARCGQPEVSWWGWRNADDEHLVQSVAKACASDSQSSVGKVSTRNSCRGFPNA
  GDLSDVEFDASLSNASGTESLALQPQKLLILDARSYAAAVANRAKGGGCECPEYYPNCEVVFMGMANIHS
  IRRSFQSLRLLCTQMPDPGNWLSALESTKWLHHLSVLLKSALLVVHAVDRDQRPVLVHCSDGWDRTPQIV
  ALAKLLLDPYYRTVEGFQVLVEMEWLDFGHKFADRCGHGENSDDLNERCPVFLQWLDCVHQLQRQFPCSF
  EFNEAFLVKLVQHTYSCLFGTFLCNNAKERGEKQTQERTCSVWSLLRAGNKAFKNLLYSSQSEAVLYPVC
  HVRNLMLWSAVYLPCPSPSTPTDDSCAPYPAPGTSPDEPPLSRLPKTRSFDNLTTTCDNMVPLASRRSSD
  PSLNEKWQEHGRSLELSSFAGSGEEVPAIDSLRRPSRLLGGAELSVAAGVAEGQMENILQEATKEESGVE
  EPTHREHTEVPEVKEEAPLAKESRTAAQGSGVLYQEPQLDDATLRSHLGPSLSSFSQGIPEHREVGHSVL
  SSSLPASLRGEDSQEVPVEQPQVENIAEDRENVVPAVPVDVKIGLGTSESSPLLPSQVPFETRGPHMNNS
  VHMLLEDKVKSESGPQLHHRPCLASSGRFSGKDMLPIAPEPRSAERPQWDSVLHRTSSPGNTLSLMMQAP
  CALPLDKCRQRIVCNGALETENKASEQPAGFDTLQKYPTPNGHCANGETGRSKDSLSHQLSATSYSSAHS
  CSRNLHHKWLNSHSGRPSTTNSPEQPSRSHLDDDGMPVYTDTIQQRLRQIESGHQQEVETLKKQVQELKS
  RLESQYLTSSLRFNGDFGDEVTSIPDSESNLDQNCLSRCSTEIFSEASWEQVDKQDTEMTRWLPDHLAAH
  CYACDSAFWLASRKHHCRNCGNVFCSSCCNQKVPVPSQQLFEPSRVCKSCYSSLHPTSSSIDLELDKPIA
  ATSN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012038
• ORF Size: 3582 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012038.1, NP_001012038.1
• RefSeq Size: 5733 bp
• RefSeq ORF: 3585 bp
• Locus ID: 305482
• Cytogenetics: 14q21
• MW: 133.5 kDa