Slc10a1 (NM_017047) Rat Tagged ORF Clone

CAT#: RR211936

Slc10a1 (Myc-DDK-tagged ORF) - Rat solute carrier family 10 (sodium/bile acid cotransporter family), member 1 (Slc10a1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc10a1
• Synonyms: Ntcp; Ntcp1; SBACT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211936 representing NM_017047
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTGCACAACGTATCAGCCCCTTTCAATTTCTCCCTGCCGCCTGGCTTTGGCCACCGGGCCACAG
  ACAAGGCGCTTAGCATCATCCTGGTGTTAATGTTGCTGCTTATCATGCTCTCACTGGGCTGCACCATGGA
  ATTCAGCAAGATCAAGGCTCACTTGTGGAAGCCCAAAGGGGTGATCGTTGCCTTGGTGGCCCAGTTTGGC
  ATCATGCCCCTCGCTGCTTTTCTTCTCGGCAAGATCTTTCACCTGAGCAACATTGAAGCTCTGGCCATCC
  TCATCTGTGGCTGCTCTCCCGGGGGGAACTTGTCCAACCTCTTCACCCTGGCCATGAAGGGGGACATGAA
  CCTCAGCATCGTGATGACCACCTGCTCCAGCTTCAGTGCCTTGGGCATGATGCCACTCCTCTTATACGTC
  TACAGCAAAGGCATCTACGATGGAGACCTTAAGGACAAGGTGCCCTACAAAGGCATTATGATATCACTAG
  TCATAGTTCTCATTCCTTGCACCATAGGGATCGTCCTCAAGTCCAAAAGGCCACACTATGTACCCTACAT
  CCTCAAGGGAGGCATGATCATCACCTTCCTCCTCTCTGTGGCTGTCACAGCCCTCTCTGTCATCAATGTG
  GGCAACAGCATCATGTTCGTCATGACACCACACTTACTGGCTACCTCCTCCCTGATGCCCTTCTCTGGCT
  TTCTGATGGGTTACATTCTCTCTGCTCTCTTCCAACTCAATCCAAGCTGCAGACGCACCATCAGCATGGA
  AACAGGATTCCAAAACATTCAACTCTGTTCTACCATCCTCAATGTGACCTTCCCCCCTGAAGTCATTGGG
  CCACTTTTCTTCTTTCCTCTCCTCTACATGATTTTCCAGCTTGCAGAAGGACTTCTCATCATCATTATCT
  TCCGGTGCTATGAGAAAATCAAGCCTCCAAAGGACCAAACAAAAATTACCTACAAAGCTGCTGCAACTGA
  GGATGCTACTCCAGCAGCTCTGGAAAAAGGTACCCACAATGGGAATATTCCTCCTCTCCAACCTGGTCCT
  TCCCCTAATGGCCTGAATTCTGGTCAGATGGCAAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211936 representing NM_017047
  Red=Cloning site Green=Tags(s)
  
  MEVHNVSAPFNFSLPPGFGHRATDKALSIILVLMLLLIMLSLGCTMEFSKIKAHLWKPKGVIVALVAQFG
  IMPLAAFLLGKIFHLSNIEALAILICGCSPGGNLSNLFTLAMKGDMNLSIVMTTCSSFSALGMMPLLLYV
  YSKGIYDGDLKDKVPYKGIMISLVIVLIPCTIGIVLKSKRPHYVPYILKGGMIITFLLSVAVTALSVINV
  GNSIMFVMTPHLLATSSLMPFSGFLMGYILSALFQLNPSCRRTISMETGFQNIQLCSTILNVTFPPEVIG
  PLFFFPLLYMIFQLAEGLLIIIIFRCYEKIKPPKDQTKITYKAAATEDATPAALEKGTHNGNIPPLQPGP
  SPNGLNSGQMAN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017047
• ORF Size: 1086 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017047.1, NP_058743.1
• RefSeq Size: 1663
• RefSeq ORF: 1089
• Locus ID: 24777
• MW: 39.3 kDa
• Gene Summary: mediates sodium dependent bile acid transport [RGD, Feb 2006]