Trh (NM_013046) Rat Tagged ORF Clone

CAT#: RR211980

Trh (Myc-DDK-tagged ORF) - Rat thyrotropin releasing hormone (Trh), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Trh
• Synonyms: Pro-TRH; THR; Trf; TRH01
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211980 representing NM_013046
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGGACCTTGGTTGCTGCTGGCTCTGGCTTTGATCTTCACCCTAACTGGTATCCCTGAATCCTGCG
  CCTTGCCGGAGGCAGCCCAGGAGGAAGGTGCAGTGACTCCTGACCTTCCTGGCCTGGAGAATGTTCAGGT
  CCGGCCAGAACGTCGATTCTTGTGGAAAGACCTCCAGCGGGTGAGAGGGGACCTCGGTGCTGCCTTAGAC
  TCCTGGATCACAAAACGCCAGCATCCAGGCAAAAGGGAGGAGGAGGAAAAAGACATTGAAGCTGAAGAGA
  GGGGAGACTTGGGAGAAGGGGGAGCCTGGAGACTCCACAAACGACAGCACCCCGGCCGACGTGCCAACCA
  GGACAAGTATTCATGGGCAGATGAGGAGGACAGTGACTGGATGCCACGGTCCTGGTTACCAGATTTCTTT
  CTGGATTCCTGGTTCTCAGATGTCCCCCAAGTCAAGCGGCAGCACCCTGGCAGGCGATCCTTCCCCTGGA
  TGGAGTCTGATGTCACCAAGAGGCAACATCCAGGCCGGAGGTTCATAGATCCCGAGCTCCAAAGAAGCTG
  GGAAGAAAAAGAGGGAGAGGGTGTCTTAATGCCTGAGAAACGCCAGCATCCTGGCAAAAGGGCATTGGGT
  CATCCCTGTGGGCCCCAGGGGACTTGTGGTCAAACAGGCCTGCTCCAGCTTCTAGGTGACCTGAGCAGGG
  GTCAGGAGACCCTGGTGAAGCAAAGCCCACAAGTGGAACCCTGGGACAAGGAACCTCTGGAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211980 representing NM_013046
  Red=Cloning site Green=Tags(s)
  
  MPGPWLLLALALIFTLTGIPESCALPEAAQEEGAVTPDLPGLENVQVRPERRFLWKDLQRVRGDLGAALD
  SWITKRQHPGKREEEEKDIEAEERGDLGEGGAWRLHKRQHPGRRANQDKYSWADEEDSDWMPRSWLPDFF
  LDSWFSDVPQVKRQHPGRRSFPWMESDVTKRQHPGRRFIDPELQRSWEEKEGEGVLMPEKRQHPGKRALG
  HPCGPQGTCGQTGLLQLLGDLSRGQETLVKQSPQVEPWDKEPLEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013046
• ORF Size: 765 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013046.1, NM_013046.2, NM_013046.3, NP_037178.1
• RefSeq Size: 1409
• RefSeq ORF: 768
• Locus ID: 25569
• MW: 29.3 kDa
• Gene Summary: This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The rat proprotein contains five thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin, and may be involved in cardiac function in heart failure. Disruption of this gene is associated with hypothyroidism, elevated thyroid-stimulating hormone levels, and hyperglycemia. [provided by RefSeq, May 2013]