Clca2 (NM_001013202) Rat Tagged ORF Clone

CAT#: RR212005

Clca2 (Myc-DDK-tagged ORF) - Rat chloride channel calcium activated 2 (Clca2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Clca2
• Synonyms: CLCA; Clca4; rbCLCA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212005 representing NM_001013202
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCCAGTGCTGAAGGTCCTTCTGTTCCTCACCCTGCACCTCTTGCAGGACACAAAAAGCTTCAAGG
  TCCATCTCAACAACAATGGATATGAGGGTGTGGTCATTGCAATTAACCCCAGTGTACCAGAGGATGAAAG
  ACTCATCCCAAGCTTAAAGGAAATGGTCACTCAAGCCTCTACTTACCTGTTTGAAGCCAGCCAAGGAAGG
  TTTTACTTCAGGAATGTAAGCATATTAGTCCCGATGACCTGGAAGTCAAAATCTGAGTACTTGATGCCAA
  AACGAGAATCATATGACAAAGCAGATGTCATAGTTGCCAATTCTCATCTGAAATATGGAGACAATCCCTA
  CACCCTTCAATATGGGCAGTGTGGGGACAGAGGACGGTACATACATTTCACTCCAAACTTCCTGCTCACT
  GATAACGTGCGTAACTATGGACCCCGAGGCAGAGTCTTTGTCCATGAGTGGGCTCATCTCCGGTGGGGAG
  TGTTTGATGAGTATAATGAGGACCGGCCTTTCTACATATCTGGAAAGAACACTATCGAAGTGACAAGGTG
  TTCTACCGACATCAAGGGCTCTAAGGCGGTCCATGAGCGCCAGAGAGGCAGCGATGTGACAAGGTTATGC
  CGATGGGACTCGAGGACAGGGCTGTATGAACCCAAATGTAAATTTTTCCCAGACAAAATTCAGACTGCCA
  GGGCCTCCATAATGTTCATGCAAAATCTCAATTCCGTGGTTGAATTTTGCACAGAAAAAACCCATAATAC
  GGAAGCCCCAAACCTACAAAACAAAATATGCAATGGCAGAAGCACGTGGGACGTAATCAAGGAGTCCGCT
  GACTTTCAGCAGGCCCCTCCCATGAGAGGAACAGAAGCCCCTCCTCCACCCACGTTTTCCTTGCTCAAGT
  CCAGACAGCGAGTGATCTGCTTGGTGCTGGATAAATCCGGAAGCATGGACACAGAAGACCGCCTTATTCG
  AATGAATCAAGCAGCGGAACTGTACTTAACTCAAATTGTTGAAAAGGAATCTATGGTTGGATTAGTCACT
  TTTGACAGCACTGCCCAAATCCAAAATTACCTGATAAAAATAACTAATACCGGTGACTACAAGAAAATCA
  CTGGAAATCTCCCTCAGCAGGCTGTTGGGGGGACTTCAATCTGCAGGGGACTGGAAGCAGGATTTCAGGC
  AATTACCTCCAGTGACCAGAGTACTTCCGGTTCTGAGATTGTATTGCTAACAGATGGGGAAGATGATCTA
  ATAAGTTCATGCTTTGAGGTGGTCAAACACAGCGGTGCGGTCATTCACACCATCGCCCTGGGGCCTAAAG
  CTGCCCGAGAACTGGAGACTTTGTCGGATATGACAGGAGGGCTTCGCTTTTATGCCAACAAAGATGTGAA
  CAGCCTGATGGACGCTTTCAGTGGGATTTCCTCTGCAAGTGGCAACCTCTCCCAGCAGGCTCTGCAGTTG
  GAGAGCAAAGCCTTCAATATTGGAGGGGGGACATGGATAAATGGTACAGTACCTGTGGACAGTACCATTG
  GCAACGACACTTTCTTTGTTATTACCTGGACAGTCCAAAAGCCAGAAATTATTCTTCGAGATCCAAAAAG
  AAAAAAATATACAACCTCAGATTTCCAAGACAATAAACTAAACATTCGATCTGCTCGACTTCAAATACCA
  GGCACTGCAGAGACAGGAACTTGGACTTACAGCCTCTTCAATAAGGGGGTCAAACCTCAGTTGCTAACTG
  TGACAGTGACCACTCGAGCCAGAAGTCCCACCACACAGCCCCTCATGGCCACCGCTCACATGAGTCAGAG
  CACAGCCCAGTACCCTAGTCCGATGATTGTGTATGCACGGGTCAGCCAAGGGTTTTTGCCTGTTCTGGGA
  GCCAATGTCACAGCCGTCATAGAATCTGAAAATGGAAGTCGAGTCACCCTGGAGCTCTGGGACAACGGGG
  CAGGTGCTGACACTGTCAAAAATGATGGCATCTACACGAGATATTTTACAGATTATCACGGAAATGGTAG
  ATACAGCTTAAAAGTGCGTGTCCAGGCAAGAAAGAGCAAAGCCAGACTAAGTTTAAGACAAAAGAACAAG
  TCTTTATATATACCTGGCTATGTTGAAAATGGTAAAATTATACTGAATCCACCCAGACCGGAAGTCCCAG
  AAGAAGCTACAGAAGCTGCAGTGGAAGACTTCAGCAGACTAACCTCTGGAGAGTCGTTTACTGTGTCCGG
  AGCGCCCCCTGATGGCGGCCACGCTCATGTGTTCCCACCCAGTAAAGTTACAGACCTGGAGGCCGAGTTT
  AAAGGTGATCATATTCACCTTACCTGGACGGCCCCTGGCAAGGTTCTCGATAAAGGAAGAGCGCACAGAT
  ACGTCATCAGAATGAGCCGGCATCCTCCTGGTCTCCAAGATTTTAACGACGCTACTTTAGTGAATACTTC
  CGGTCTGATACCTAAAGAGGCTGGCTCAGAAGAAACGTTTAAATTCAAACCAGCAAGTTTTAAAATAGAA
  AACGGCACCCAGCTCTACATTGCAATTCTGGCCAACAATGAAGCCGGTCTCACCTCTGAGGTCTCCAACA
  TCGCACAGGCTGTCAACTTTATTCCTCCAGAACATAGTTTCTCTACACTCAGCCCTGATATTTCTGCAAT
  CTGTCTGACAATTTGGGGGTTAACTGTGATTCTTAACGATATTTTAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212005 representing NM_001013202
  Red=Cloning site Green=Tags(s)
  
  MVPVLKVLLFLTLHLLQDTKSFKVHLNNNGYEGVVIAINPSVPEDERLIPSLKEMVTQASTYLFEASQGR
  FYFRNVSILVPMTWKSKSEYLMPKRESYDKADVIVANSHLKYGDNPYTLQYGQCGDRGRYIHFTPNFLLT
  DNVRNYGPRGRVFVHEWAHLRWGVFDEYNEDRPFYISGKNTIEVTRCSTDIKGSKAVHERQRGSDVTRLC
  RWDSRTGLYEPKCKFFPDKIQTARASIMFMQNLNSVVEFCTEKTHNTEAPNLQNKICNGRSTWDVIKESA
  DFQQAPPMRGTEAPPPPTFSLLKSRQRVICLVLDKSGSMDTEDRLIRMNQAAELYLTQIVEKESMVGLVT
  FDSTAQIQNYLIKITNTGDYKKITGNLPQQAVGGTSICRGLEAGFQAITSSDQSTSGSEIVLLTDGEDDL
  ISSCFEVVKHSGAVIHTIALGPKAARELETLSDMTGGLRFYANKDVNSLMDAFSGISSASGNLSQQALQL
  ESKAFNIGGGTWINGTVPVDSTIGNDTFFVITWTVQKPEIILRDPKRKKYTTSDFQDNKLNIRSARLQIP
  GTAETGTWTYSLFNKGVKPQLLTVTVTTRARSPTTQPLMATAHMSQSTAQYPSPMIVYARVSQGFLPVLG
  ANVTAVIESENGSRVTLELWDNGAGADTVKNDGIYTRYFTDYHGNGRYSLKVRVQARKSKARLSLRQKNK
  SLYIPGYVENGKIILNPPRPEVPEEATEAAVEDFSRLTSGESFTVSGAPPDGGHAHVFPPSKVTDLEAEF
  KGDHIHLTWTAPGKVLDKGRAHRYVIRMSRHPPGLQDFNDATLVNTSGLIPKEAGSEETFKFKPASFKIE
  NGTQLYIAILANNEAGLTSEVSNIAQAVNFIPPEHSFSTLSPDISAICLTIWGLTVILNDILN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013202
• ORF Size: 2709 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013202.1, NM_001013202.2, NP_001013220.1
• RefSeq Size: 3324 bp
• RefSeq ORF: 2712 bp
• Locus ID: 362052
• Cytogenetics: 2q44
• MW: 100.3 kDa
• Gene Summary: calcium-sensitive chloride channel which is expressed in endothelial cells [RGD, Feb 2006]