Mthfd1 (NM_022508) Rat Tagged ORF Clone

CAT#: RR212063

Mthfd1 (Myc-DDK-tagged ORF) - Rat methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (Mthfd1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mthfd1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212063 representing NM_022508
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACCAGCGGGAATCCTGAACGGGAAAGTGGTCTCCGCGCAAATCAGGAATCGACTGAAGACCCAGG
  TTACTCAGATGCAGGAGCAGGTACCTGGCTTCACCCCTGGCCTGGCAATCCTGCAGGTTGGCGACAGAGA
  TGATTCCAATCTTTATATAAATGTGAAGCTGAAGGCTGCTCAAGAGATCGGGATCAAAGCCACTCACATT
  AAATTACCAAGAACTTCCACAGAGTCGGAGGTGTTAAAATACGTCATCTCCCTGAATGAGGATGCCACTG
  TGCACGGATTCATAGTACAGCTGCCTTTAGACTCCGAGAATTCCATTAACACAGAGGCAGTCATCAATGC
  CATTGCCCCTGAGAAGGATGTGGACGGGTTGACGAGCATCAATGCCGGGAAGCTTGCCAGAGGTGATCTA
  AAGGACTGCTTCATTCCGTGCACACCCAAAGGATGCTTGGAACTCATCAAAGAGACAGGAGTGCAGATTG
  CAGGAAGGCACGCCGTGGTGGTCGGGCGAAGTAAAATAGTTGGTGCACCCATGCACGACCTGCTTCTGTG
  GAACAATGCCACGGTGACCACCTGCCACTCCAAGACTGCCGATCTGGACAAGGAGGTAAATAAAGGAGAC
  ATTCTGGTGGTTGCAACAGGACAGCCTGAGATGGTGAAAGGGGAGTGGATCAAGCCTGGAGCTGTGGTCA
  TAGACTGTGGGATCAATTATGTTCCAGATGATACAAAACCAAATGGAAGGAAAGTTGTGGGTGACGTAGC
  ATATGACGAGGCCAAGGAGAAGGCGAGCTTTATCACGCCTGTCCCCGGTGGCGTGGGGCCCATGACCGTG
  GCAATGCTGATGCAGAGCACAGTAGAGAGCGCACAGCGCTTCCTGAAGAAATTTAAGCCAGGGAAGTGGA
  CAATTCAGTATAACAAGCTGAACCTCAAGACGCCTGTACCAAGTGACATTGCTATATCACGATCTTGCAA
  ACCCAAGCTCATCGGTAACCTGGCCCGAGAAATTGGGCTACTCACTGAGGAGGTGGAATTGTATGGAGAA
  ACAAAGGCCAAGGTCTTACTGTCAGCACTAGATCGCCTGAAGCATCAGCCAGATGGGAAATACGTTGTGG
  TGACTGGAATTACTCCAACACCCCTGGGAGAAGGGAAGAGCACAACCACCATCGGGCTTGTGCAAGCCCT
  TGGCGCCCACCTGCATCAGAATGTTTTTGCGTGTGTGCGACAGCCTTCTCAGGGCCCCACCTTTGGGATA
  AAAGGTGGCGCTGCAGGAGGCGGCTATTCCCAGGTCATTCCTATGGAAGAGTTTAATCTCCACCTCACTG
  GTGACATCCACGCCATCACTGCCGCTAATAACCTTGTGGCTGCTGCTATTGACGCTCGGATATTTCATGA
  GCTGACCCAGACAGACAAGGCTCTCTTTAATCGTCTGGTACCCTCAGTAAATGGAGTAAGAAAGTTCTCT
  GACATCCAAATCCGCAGGTTACGGAGGCTAGGCATTGAGAAGACTGACCCCGCTGCGCTGACAGATGATG
  AGATAAACAGATTTGCAAGACTAGATATTGATCCAGAAACCATAACATGGCAGAGAGTGCTGGATACTAA
  CGATAGATTCCTGAGGAAGATCACCATTGGACAGGCTCCAACCGAGAAAGGCCACACACGCACGGCCCAG
  TTTGATATCTCTGTGGCCAGTGAAATCATGGCAGTCCTGGCCCTCACTAGTTCTCTGGAAGACATGAGAG
  CAAGGCTGGGCAAAATGGTGGTAGCGTCCAGTAAGAAAGGGGAGCCTATCAGCTGTGAAGACCTGGGCGT
  GAGCGGGGCGCTGACGGTGCTCATGAAGGATGCGATCAAGCCCAATCTCATGCAGACCTTAGAGGGCACG
  CCGGTATTTGTCCATGCTGGGCCTTTTGCCAACATTGCACATGGGAATTCCTCCATCATTGCAGACCGGA
  TTGCACTCAAGCTGGTTGGCCCTGAGGGCTTTGTAGTGACAGAAGCAGGATTCGGAGCAGACATAGGAAT
  GGAAAAGTTCTTCAACATCAAGTGCCGGTATTCTGGTCTCCAGCCTCATGTGGTGGTTCTTGTTGCCACT
  GTCAGGGCTCTTAAGATGCACGGGGGTGGCCCCACGGTCACCGCTGGACTGCCTCTTCCCAAGGCTTACA
  CAGAAGAGGACCTGGACCTGGTGGAAAAGGGCTTCAGTAACTTGAGGAAACAGATAGAAAATGCTAGAAT
  GTTTGGAGTGCCTGTCGTGGTGGCCATGAATGCATTCAAGACAGATACAGATACTGAGCTGGACCTCATC
  GGCCGCCTCTCCAGAGAGCATGGGGCTTTCGATGCTGTCAAGTGCACCCACTGGGCAGAAGGGGGCCAGG
  GAGCCTTAGCCCTGGCTCAGGCTGTCCAGAGAGCTTCACAGGCCCCCAGCAGCTTCCAGCTCCTCTATGA
  CCTCAAGCTCTCAGTTGAAGATAAAATCAGGATTATTGCACAGAAGATCTACGGGGCCGATGACATCGAA
  TTGCTCCCTGAAGCACAAAACAAAGCAGAAATCTACACAAAGCAGGGCTTTGGGAATCTACCCATCTGCA
  TGGCCAAAACACACTTGTCTTTATCTCACAACCCAGAGCAAAAAGGGGTGCCTACTGGCTTCGTTCTGCC
  CATCCGGGACATCCGGGCCAGCGTTGGGGCTGGTTTCCTGTACCCTTTAGTAGGAACGATGAGCACAATG
  CCTGGACTCCCTACTCGACCCTGTTTTTATGATATCGATTTGGACCCTGAAACTGAACAAGTGAATGGAT
  TGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212063 representing NM_022508
  Red=Cloning site Green=Tags(s)
  
  MAPAGILNGKVVSAQIRNRLKTQVTQMQEQVPGFTPGLAILQVGDRDDSNLYINVKLKAAQEIGIKATHI
  KLPRTSTESEVLKYVISLNEDATVHGFIVQLPLDSENSINTEAVINAIAPEKDVDGLTSINAGKLARGDL
  KDCFIPCTPKGCLELIKETGVQIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTADLDKEVNKGD
  ILVVATGQPEMVKGEWIKPGAVVIDCGINYVPDDTKPNGRKVVGDVAYDEAKEKASFITPVPGGVGPMTV
  AMLMQSTVESAQRFLKKFKPGKWTIQYNKLNLKTPVPSDIAISRSCKPKLIGNLAREIGLLTEEVELYGE
  TKAKVLLSALDRLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGI
  KGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARIFHELTQTDKALFNRLVPSVNGVRKFS
  DIQIRRLRRLGIEKTDPAALTDDEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAPTEKGHTRTAQ
  FDISVASEIMAVLALTSSLEDMRARLGKMVVASSKKGEPISCEDLGVSGALTVLMKDAIKPNLMQTLEGT
  PVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLQPHVVVLVAT
  VRALKMHGGGPTVTAGLPLPKAYTEEDLDLVEKGFSNLRKQIENARMFGVPVVVAMNAFKTDTDTELDLI
  GRLSREHGAFDAVKCTHWAEGGQGALALAQAVQRASQAPSSFQLLYDLKLSVEDKIRIIAQKIYGADDIE
  LLPEAQNKAEIYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFVLPIRDIRASVGAGFLYPLVGTMSTM
  PGLPTRPCFYDIDLDPETEQVNGLF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022508
• ORF Size: 2805 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022508.1, NP_071953.1
• RefSeq Size: 3332
• RefSeq ORF: 2808
• Locus ID: 64300
• MW: 101 kDa
• Gene Summary: a trifunctional enzyme combining a 10-formyl-H4folate synthetase, a 5,10-methenyl-H4folate cyclohydrolase, and a 5,10-methylene-H4folate dehydrogenase [RGD, Feb 2006]