Eif4e (NM_053974) Rat Tagged ORF Clone

CAT#: RR212121

Eif4e (Myc-DDK-tagged ORF) - Rat eukaryotic translation initiation factor 4E (Eif4e), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eif4e
• Synonyms: MGC93265
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212121 representing NM_053974
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACTGTGGAACCGGAAACCACCCCTACCACTAATCCCCCACCTGCGGAAGAGGAAAAAACAGAGT
  CTAATCAGGAGGTTGCTAATCCAGAGCACTATATTAAACACCCTCTACAGAACAGGTGGGCACTCTGGTT
  TTTTAAAAATGATAAAAGCAAAACTTGGCAAGCAAACCTTCGGTTGATCTCTAAGTTTGATACTGTTGAA
  GACTTTTGGGCTCTATACAACCATATCCAGTTGTCTAGTAATTTAATGCCTGGCTGTGACTACTCACTTT
  TTAAGGATGGTATTGAGCCTATGTGGGAAGATGAGAAAAACAAACGGGGAGGACGATGGCTAATTACATT
  GAACAAGCAGCAGAGACGAAGTGACCTCGATCGCTTTTGGCTAGAGACACTGCTGTGCCTTATTGGAGAA
  TCTTTTGATGACTACAGTGATGACGTGTGTGGAGCTGTTGTCAATGTTAGAGCTAAAGGTGATAAGATAG
  CAATATGGACGACTGAATGTGAAAACAGAGATGCAGTCACACACATAGGGAGGGTATACAAGGAAAGGTT
  AGGACTTCCTCCGAAGATAGTGATTGGTTATCAGTCCCACGCAGACACAGCTACAAAGAGCGGCTCCACC
  ACTAAAAATAGGTTTGTTGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212121 representing NM_053974
  Red=Cloning site Green=Tags(s)
  
  MATVEPETTPTTNPPPAEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVE
  DFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGE
  SFDDYSDDVCGAVVNVRAKGDKIAIWTTECENRDAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGST
  TKNRFVV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053974
• ORF Size: 651 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053974.1, NM_053974.2, NP_446426.1
• RefSeq Size: 2395
• RefSeq ORF: 654
• Locus ID: 117045
• MW: 25.1 kDa
• Gene Summary: binds to the mRNA 7-methylguanosine cap and mediates mRNA binding to the 40S ribosome in the rate limiting step in translational initiation [RGD, Feb 2006]