Cps1 (NM_017072) Rat Tagged ORF Clone

CAT#: RR212123

Cps1 (Myc-DDK-tagged ORF) - Rat carbamoyl-phosphate synthetase 1, mitochondrial (Cps1), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cps1
• Synonyms: cps; cps-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212123 representing NM_017072
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGAGGATTTTGACAGCATGCAAAGTGGTGAAGACACTGAAGAGTGGCTTTGGTTTGGCCAATGTGA
  CTTCGAAGCGACAGTGGGACTTCTCTAGACCTGGCATCAGGCTCCTTTCTGTGAAGGCACAGACAGCACA
  CATTGTTCTGGAAGATGGAACTAAGATGAAGGGCTACTCCTTTGGCCATCCCTCCTCGGTTGCTGGCGAA
  GTGGTTTTTAATACTGGCTTAGGAGGGTACTCGGAAGCACTTACTGATCCTGCCTACAAGGGGCAGATCC
  TCACCATGGCCAACCCTATCATTGGGAATGGTGGGGCCCCTGATACAACGGCACGTGATGAACTGGGACT
  GAATAAGTACATGGAGTCTGATGGAATCAAGGTGGCGGGTCTGCTGGTGCTGAATTACAGTCATGACTAC
  AACCACTGGCTGGCCACCAAGAGTCTGGGTCAGTGGCTGCAGGAGGAGAAGGTCCCTGCAATTTATGGAG
  TGGATACAAGAATGCTGACTAAAATAATTCGGGATAAGGGTACCATGCTTGGGAAGATTGAGTTTGAGGG
  CCAGTCTGTGGACTTTGTGGATCCTAATAAGCAGAATTTGATTGCCGAGGTTTCAACCAAGGATGTCAAG
  GTGTTTGGCAAAGGAAACCCCACGAAAGTGGTAGCCGTGGACTGTGGGATAAAAAACAATGTCATCCGCC
  TGCTAGTTAAGCGAGGAGCGGAAGTGCATTTGGTCCCCTGGAATCATGACTTCACCCAGATGGACTATGA
  CGGACTTCTGATCGCTGGAGGACCTGGGAACCCAGCTCTGGCACAGCCACTAATTCAGAACGTGAAGAAG
  ATTTTGGAGAGTGACCGCAAAGAGCCGTTGTTTGGAATCAGTACAGGAAACATAATAACAGGATTGGCTG
  CTGGCGCCAAATCCTACAAGATGTCCATGGCCAACAGAGGACAGAACCAACCTGTTTTGAATATCACAAA
  CAGACAGGCTTTCATAACTGCTCAGAATCATGGCTATGCTCTGGACAACACCCTCCCTGCTGGCTGGAAA
  CCACTGTTTGTCAATGTCAATGACCAAACAAATGAGGGGATTATGCATGAGAGCAAACCCTTCTTCGCTG
  TGCAGTTCCACCCAGAGGTCAGCCCGGGGCCAACAGACACTGAGTACCTATTTGATTCCTTCTTCTCGCT
  GATAAAGAAGGGCAAAGGCACCACCATTACCTCAGTTCTGCCCAAGCCAGCATTGGTGGCATCTCGAGTC
  GAGGTTTCCAAGGTCCTTATCCTAGGATCAGGAGGCCTGTCCATTGGTCAGGCAGGTGAATTTGATTACT
  CCGGATCTCAGGCTGTAAAAGCCATGAAGGAAGAAAACGTCAAAACAGTCCTGATGAACCCGAACATTGC
  ATCCGTGCAGACCAACGAGGTGGGATTGAAGCAGGCAGATGCAGTCTACTTTCTCCCTATCACCCCCCAG
  TTTGTCACAGAAGTTATCAAGGCTGAACGGCCCGATGGGTTAATTCTGGGCATGGGTGGCCAGACAGCTC
  TGAACTGTGGAGTGGAACTATTCAAGAGGGGTGTGCTCAAGGAATATGGTGTGAAGGTCTTGGGAACATC
  GGTCGAGTCCATTATGGCCACAGAAGACAGGCAGCTTTTCTCAGACAAGCTGAATGAGATCAACGAGAAG
  ATTGCTCCTAGCTTTGCAGTGGAATCAATGGAGGATGCCTTGAAGGCAGCAGACACCATTGGCTACCCTG
  TGATGATTCGGTCTGCGTATGCTCTGGGTGGGTTAGGCTCCGGCATCTGTCCCAACAAGGAGACCTTAAT
  GGATCTTGGCACAAAGGCATTTGCTATGACCAACCAGATCCTGGTGGAGCGGTCAGTGACAGGTTGGAAA
  GAAATAGAATATGAAGTTGTCCGAGATGCGGATGATAACTGTGTCACAGTCTGTAACATGGAGAATGTTG
  ACGCCATGGGTGTTCACACAGGTGATTCAGTTGTTGTGGCCCCGGCCCAGACCCTCTCCAATGCAGAGTT
  CCAGATGTTGAGACGCACTTCAATCAATGTTGTTCGCCACTTGGGCATTGTGGGTGAATGCAACATTCAG
  TTTGCTCTTCACCCCACTTCCATGGAATACTGTATCATTGAAGTGAATGCCAGGCTCTCCAGGAGTTCTG
  CCCTGGCCTCCAAGGCCACTGGCTACCCACTGGCGTTCATCGCAGCAAAGATCGCTCTAGGAATCCCACT
  TCCAGAAATCAAGAATGTTGTGTCTGGGAAGACCTCAGCCTGCTTCGAACCTAGCCTGGATTACATGGTG
  ACCAAGATTCCTCGCTGGGACCTTGACCGTTTTCATGGAACATCCAGCCGAATTGGTAGCTCAATGAAAA
  GTGTAGGCGAGGTCATGGCCATTGGTCGCACCTTTGAGGAAAGTTTCCAGAAGGCTCTGAGGATGTGCCA
  TCCATCTGTGGATGGGTTCACTCCCCGTCTCCCAATGAATAAGGAATGGCCAGCAAACCTGGATCTGAGG
  AAGGAGCTGTCTGAACCCTCCAGCACCCGCATCTATGCCATTGCTAAGGCCTTGGAAAACAACATGTCCC
  TTGACGAAATCGTGAAGCTCACATCCATTGACAAGTGGTTTTTGTATAAGATGCGTGACATTTTAAACAT
  GGATAAGACACTGAAAGGGCTTAACAGTGAGTCTGTTACAGAAGAAACTCTGAGACAGGCCAAAGAGATT
  GGGTTCTCAGACAAGCAGATTTCAAAATGTTTAGGACTGACCGAGGCTCAGACAAGAGAGCTGAGATTGA
  AGAAAAACATCCACCCTTGGGTTAAACAGATTGATACATTGGCTGCAGAATACCCATCAGTGACAAATTA
  CCTGTATGTTACCTACAATGGCCAGGAGCACGACATCAAATTTGATGAACATGGAATAATGGTGCTGGGC
  TGTGGCCCATACCACATTGGCAGCAGTGTGGAATTTGATTGGTGTGCTGTCTCCAGTATCCGCACACTGC
  GCCAACTTGGCAAGAAGACGGTGGTGGTGAATTGTAACCCGGAGACTGTGAGCACTGACTTTGATGAGTG
  TGACAAACTCTACTTTGAAGAGCTGTCTTTGGAGAGAATCCTAGATATCTACCACCAGGAGGCATGTAAT
  GGCTGCATCATATCAGTCGGGGGCCAGATTCCAAACAACTTGGCGGTTCCGCTATACAAGAACGGTGTCA
  AGATCATGGGTACCAGTCCTCTGCAGATCGATAGGGCTGAGGATCGCTCCATCTTCTCGGCTGTCTTAGA
  TGAGCTGAAGGTGGCCCAGGCTCCTTGGAAAGCTGTTAACACTTTGAACGAGGCGCTGGAATTTGCAAAC
  TCTGTGGGCTATCCCTGCTTACTGAGACCTTCCTATGTTTTGAGTGGGTCTGCCATGAACGTGGTATTCT
  CTGAGGATGAGATGAAAAGGTTCCTTGAGGAGGCCACTCGAGTCTCTCAGGAACACCCAGTGGTGCTGAC
  CAAGTTTATTGAGGGGGCTCGGGAAGTGGAGATGGACGCTGTTGGCAAAGAAGGACGGGTCATCTCCCAT
  GCCATCTCTGAACATGTTGAAGATGCAGGTGTCCACTCAGGGGATGCCACACTGATGCTACCTACGCAGA
  CCATCAGCCAAGGAGCCATTGAAAAGGTGAAGGATGCCACACGAAAGATTGCAAAGGCTTTTGCCATCTC
  TGGGCCATTCAATGTTCAGTTTCTTGTCAAAGGAAATGATGTCTTGGTGATTGAGTGCAATCTGAGAGCC
  TCTCGATCCTTCCCCTTTGTCTCCAAGACTCTTGGGGTGGACTTCATTGATGTGGCCACCAAGGTGATGA
  TCGGAGAGAGTGTTGATGAGAAGCATCTACCCACACTGGAACAACCCATCATCCCCTCTGACTATGTTGC
  CATTAAGGCTCCCATGTTTTCCTGGCCCCGACTGAGGGATGCTGATCCTATTCTGAGATGTGAGATGGCT
  TCTACTGGAGAGGTGGCGTGTTTTGGTGAGGGCATTCATACAGCCTTCCTAAAGGCAATGCTGTCCACAG
  GGTTTAAGATACCTCAGAAGGGCATTCTGATTGGCATCCAGCAATCATTCCGTCCAAGATTCCTTGGTGT
  TGCTGAGCAGTTACACAATGAAGGTTTCAAGCTTTTTGCCACAGAAGCCACATCAGACTGGCTCAACGCC
  AACAATGTTCCTGCCACCCCAGTGGCTTGGCCATCTCAGGAAGGACAGAATCCCAGCCTCTCTTCCATCA
  GAAAGTTGATAAGAGACGGAAGCATTGACCTAGTGATTAACCTCCCCAATAACAACACCAAATTTGTCCA
  TGATAATTATGTGATTCGGAGGACAGCTGTGGACAGTGGAATTGCTCTGCTCACCAATTTCCAGGTGACC
  AAACTTTTTGCTGAGGCTGTGCAGAAAGCTCGTACTGTGGACTCCAAGAGTCTGTTCCACTACAGGCAGT
  ACAGCGCTGGAAAGGCAGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212123 representing NM_017072
  Red=Cloning site Green=Tags(s)
  
  MTRILTACKVVKTLKSGFGLANVTSKRQWDFSRPGIRLLSVKAQTAHIVLEDGTKMKGYSFGHPSSVAGE
  VVFNTGLGGYSEALTDPAYKGQILTMANPIIGNGGAPDTTARDELGLNKYMESDGIKVAGLLVLNYSHDY
  NHWLATKSLGQWLQEEKVPAIYGVDTRMLTKIIRDKGTMLGKIEFEGQSVDFVDPNKQNLIAEVSTKDVK
  VFGKGNPTKVVAVDCGIKNNVIRLLVKRGAEVHLVPWNHDFTQMDYDGLLIAGGPGNPALAQPLIQNVKK
  ILESDRKEPLFGISTGNIITGLAAGAKSYKMSMANRGQNQPVLNITNRQAFITAQNHGYALDNTLPAGWK
  PLFVNVNDQTNEGIMHESKPFFAVQFHPEVSPGPTDTEYLFDSFFSLIKKGKGTTITSVLPKPALVASRV
  EVSKVLILGSGGLSIGQAGEFDYSGSQAVKAMKEENVKTVLMNPNIASVQTNEVGLKQADAVYFLPITPQ
  FVTEVIKAERPDGLILGMGGQTALNCGVELFKRGVLKEYGVKVLGTSVESIMATEDRQLFSDKLNEINEK
  IAPSFAVESMEDALKAADTIGYPVMIRSAYALGGLGSGICPNKETLMDLGTKAFAMTNQILVERSVTGWK
  EIEYEVVRDADDNCVTVCNMENVDAMGVHTGDSVVVAPAQTLSNAEFQMLRRTSINVVRHLGIVGECNIQ
  FALHPTSMEYCIIEVNARLSRSSALASKATGYPLAFIAAKIALGIPLPEIKNVVSGKTSACFEPSLDYMV
  TKIPRWDLDRFHGTSSRIGSSMKSVGEVMAIGRTFEESFQKALRMCHPSVDGFTPRLPMNKEWPANLDLR
  KELSEPSSTRIYAIAKALENNMSLDEIVKLTSIDKWFLYKMRDILNMDKTLKGLNSESVTEETLRQAKEI
  GFSDKQISKCLGLTEAQTRELRLKKNIHPWVKQIDTLAAEYPSVTNYLYVTYNGQEHDIKFDEHGIMVLG
  CGPYHIGSSVEFDWCAVSSIRTLRQLGKKTVVVNCNPETVSTDFDECDKLYFEELSLERILDIYHQEACN
  GCIISVGGQIPNNLAVPLYKNGVKIMGTSPLQIDRAEDRSIFSAVLDELKVAQAPWKAVNTLNEALEFAN
  SVGYPCLLRPSYVLSGSAMNVVFSEDEMKRFLEEATRVSQEHPVVLTKFIEGAREVEMDAVGKEGRVISH
  AISEHVEDAGVHSGDATLMLPTQTISQGAIEKVKDATRKIAKAFAISGPFNVQFLVKGNDVLVIECNLRA
  SRSFPFVSKTLGVDFIDVATKVMIGESVDEKHLPTLEQPIIPSDYVAIKAPMFSWPRLRDADPILRCEMA
  STGEVACFGEGIHTAFLKAMLSTGFKIPQKGILIGIQQSFRPRFLGVAEQLHNEGFKLFATEATSDWLNA
  NNVPATPVAWPSQEGQNPSLSSIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIALLTNFQVT
  KLFAEAVQKARTVDSKSLFHYRQYSAGKAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017072
• ORF Size: 4500 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017072.1, NM_017072.2, NP_058768.1
• RefSeq Size: 4503
• RefSeq ORF: 4503
• Locus ID: 497840
• MW: 164.6 kDa
• Gene Summary: catalyzes synthesis of carbamyl phosphate from NH3, HCO3, and 2ATP [RGD, Feb 2006]