Selenoi (NM_001134754) Rat Tagged ORF Clone

CAT#: RR212151

Seli (Myc-DDK-tagged ORF) - Rat selenoprotein I (Seli), (10 ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Symbol: Selenoi
• Synonyms: Ept1; RGD1560938; Seli
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212151 representing NM_001134754
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGCTACGAATACGTGAGCCCAGAGCAGCTGGCGGGCTTTGACAAGTATAAGTACAGCGCTTTGG
  ATACCAACCCACTCTCTCTGTATGTCATGCACCCATTTTGGAACACTATAGTGAAGGTATTTCCCACTTG
  GCTGGCTCCCAATCTTATAACCTTTTCTGGCTTTATGCTGCTTGTGTTCAATTTCCTACTCCTGACATAC
  TTCGACCCTGACTTCTATGCTTCAGCGCCAGGTCATAAGCATGTACCTGATTGGGTCTGGATCGTTGTGG
  GCATCCTCAACTTCGTTGCCTACACTCTAGATGGAGTAGATGGAAAACAAGCTCGTAGAACTAATTCCAG
  CACCCCATTAGGGGAGCTGTTTGACCACGGCCTGGACAGTTGGGCATGCGTTTATTTCGTTGTGACTGTG
  TACTCTATCTTTGGACGAGGACCGACTGGCGTCAGTGTTTTTGTTCTTTACCTCCTGCTGTGGGTGGTTT
  TGTTTTCTTTTATCCTGTCTCACTGGGAGAAATATAACACAGGCGTTCTTTTCCTGCCATGGGGATATGA
  CATTAGCCAAGTGACTATTTCTTTTGTCTACATAGTGACTGCGGTTGTGGGAGTTGAGGCCTGGTATGAA
  CCTTTCCTGTTTAATTTCTTATATAGAGACCTATTCACTGCAATGATTATTGGATGTGCATTGTGTGTGA
  CTCTTCCAATGAGTTTATTAAACTTTTATAGAAGCTATAAAAGCAACTCTCTGAAGCACAATTCCATCTA
  TGAAGCCATGGTCCCCTTCTTCTCTCCATGCCTGCTCTTCACTCTGTCGACAGTGTGGATCCTCCGGTCA
  CCTTCAGATATTTTAGAAATGCACCCTAGAATATTCTACTTCATGGTCGGAACAGCTTTTGCCAATATCA
  CATGTCAGCTAATCGTTTGCCAAATGAGCAGCACACGGTGCCCAACTTTGAACTGGTTGCTGCTTCCTCT
  CTTCTTGGTCGTGGCAACTGTGATCGTAGGCACAACAACCTCCCGCCTTGAGAGTGTTCTCCTTTACACA
  CTCACTGCTGCCTTCACTCTGGCTCACATCCATTATGGAGTACAAGTGGTAAAGCAGCTGAGCCGACATT
  TTCAGATTTATCCTTTTTCATTGAGGAAACCAAACTCAGATTGACTAGGACTGGAAGAGAAGAGTATCGG
  TCCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212151 representing NM_001134754
  Red=Cloning site Green=Tags(s)
  
  MAGYEYVSPEQLAGFDKYKYSALDTNPLSLYVMHPFWNTIVKVFPTWLAPNLITFSGFMLLVFNFLLLTY
  FDPDFYASAPGHKHVPDWVWIVVGILNFVAYTLDGVDGKQARRTNSSTPLGELFDHGLDSWACVYFVVTV
  YSIFGRGPTGVSVFVLYLLLWVVLFSFILSHWEKYNTGVLFLPWGYDISQVTISFVYIVTAVVGVEAWYE
  PFLFNFLYRDLFTAMIIGCALCVTLPMSLLNFYRSYKSNSLKHNSIYEAMVPFFSPCLLFTLSTVWILRS
  PSDILEMHPRIFYFMVGTAFANITCQLIVCQMSSTRCPTLNWLLLPLFLVVATVIVGTTTSRLESVLLYT
  LTAAFTLAHIHYGVQVVKQLSRHFQIYPFSLRKPNSD*LGLEEKSIGP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001134754
• ORF Size: 1194 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info The expression of this clone is not guaranteed due to the nature of selenoproteins.
• OTI Annotation: This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.

Reference Data

• RefSeq: NM_001134754.1, NP_001128226.2
• RefSeq Size: 2636
• RefSeq ORF: 1197
• Locus ID: 362713
• MW: 45.4 kDa
• Gene Summary: The multi-pass transmembrane protein encoded by this gene belongs to the CDP-alcohol phosphatidyltransferase class-I family. It catalyzes the transfer of phosphoethanolamine from CDP-ethanolamine to diacylglycerol to produce phosphatidylethanolamine, which is involved in the formation and maintenance of vesicular membranes, regulation of lipid metabolism, and protein folding. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2016]