St8sia4 (NM_053914) Rat Tagged ORF Clone

CAT#: RR212164

St8sia4 (Myc-DDK-tagged ORF) - Rat ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 (St8sia4), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: St8sia4
• Synonyms: Siat8d
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212164 representing NM_053914
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACAAGGTGTCACATTTGAAAAGAAACCCGAGCCCCAGGGAGGCGGCACAGAGCGACCCCAGCCCGG
  CTAGCGCAAACTGCGCGGCAGGGCGCTGGGCAGCGCTGGAGAACCGGGAGAGCTCCACCGCTGAACGTCC
  TAGCGACCTGATTCTGGGATCTCGGCTCCACGCTCCCTTCGCAAATTTCAGATTTCTCCCCCTTGATTAT
  TTCCCCAAAACGGAGCCTTTATACCAGGAGAAGGTGCCTGAGCTGGGACAACCTGGACTTTCCCGGGCAC
  CCAAGATGCGCTCCATTAGAAAACGGTGGACCATCTGCACTATAAGTCTACTCCTGATCTTTTATAAGAC
  AAAAGAAATAGCCAGGACTGAGGAGCACCAAGAGACGCAACTCATCGGAGATGGTGAATTATGCTTGAGC
  AGATCACTTGTCAACAACTCTGATAAAATCACTCGGAAGGCTGGTTCCACCATCTTCCAACATTCCGTAC
  AAGGCTGGAAAATCAATTCTTCTTTAGTCCTGGAGATAAGGAAGAATATTCTCCGTTTCCTAGACGCAGA
  ACGTGACGTGTCGGTGGTCAAGAGCAGTTTCAAGCCTGGTGATGTCATACACTATGTGCTGGACAGACGC
  CGGACACTAAATATTTCCCACGATCTGCATAGCCTCCTGCCTGAAGTTTCACCAATGAAAAACCGCAGGT
  TTAAGACCTGTGCTGTTGTTGGAAATTCCGGCATTCTACTAGACAGTGGATGTGGAAAGGAGATCGACAG
  CCACAACTTTGTCATAAGGTGTAATCTAGCTCCCGTGGTGGAGTTCGCTGCGGATGTGGGGACTAAGTCA
  GATTTCATTACCATGAATCCATCAGTTGTGCAAAGAGCATTCGGAGGCTTTCGGAATGAGAGTGACAGAG
  AAAAATTTGTGCACAGACTCTCCATGCTGAATGACAGCGTCCTTTGGATTCCTGCCTTCATGGTCAAAGG
  AGGAGAGAAGCACGTGGAATGGGTTAATGCATTAATCCTTAAGAATAAACTGAAAGTGAGGACTGCCTAT
  CCATCACTGAGACTCATTCATGCGGTCAGAGGTTACTGGCTGACCAACAAAGTTCCTATCAAAAGACCCA
  GCACTGGTCTCCTCATGTACACCCTTGCCACCAGATTCTGTGACGAAATTCACCTGTATGGATTCTGGCC
  ATTTCCTAAGGATCTGAACGGAAAAGCTGTCAAATATCATTACTATGATGACTTGAAATATAGATACTTT
  TCCAACGCAAGTCCTCACAGAATGCCACTGGAATTCAAAACATTGAATGTGCTGCACAATAGAGGAGCTC
  TAAAACTGACCACAGGGAAGTGCATGAAGCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212164 representing NM_053914
  Red=Cloning site Green=Tags(s)
  
  MHKVSHLKRNPSPREAAQSDPSPASANCAAGRWAALENRESSTAERPSDLILGSRLHAPFANFRFLPLDY
  FPKTEPLYQEKVPELGQPGLSRAPKMRSIRKRWTICTISLLLIFYKTKEIARTEEHQETQLIGDGELCLS
  RSLVNNSDKITRKAGSTIFQHSVQGWKINSSLVLEIRKNILRFLDAERDVSVVKSSFKPGDVIHYVLDRR
  RTLNISHDLHSLLPEVSPMKNRRFKTCAVVGNSGILLDSGCGKEIDSHNFVIRCNLAPVVEFAADVGTKS
  DFITMNPSVVQRAFGGFRNESDREKFVHRLSMLNDSVLWIPAFMVKGGEKHVEWVNALILKNKLKVRTAY
  PSLRLIHAVRGYWLTNKVPIKRPSTGLLMYTLATRFCDEIHLYGFWPFPKDLNGKAVKYHYYDDLKYRYF
  SNASPHRMPLEFKTLNVLHNRGALKLTTGKCMKQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053914
• ORF Size: 1362 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053914.1, NP_446366.1
• RefSeq Size: 1365
• RefSeq ORF: 1365
• Locus ID: 116696
• MW: 51.8 kDa
• Gene Summary: enzyme involved in polysialylation of N-CAM [RGD, Feb 2006]