Kpna5 (NM_001025113) Rat Tagged ORF Clone

CAT#: RR212168

Kpna5 (Myc-DDK-tagged ORF) - Rat karyopherin alpha 5 (importin alpha 6) (Kpna5), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kpna5
• Synonyms: Ipoa6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212168 representing NM_001025113
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAGTCCTGGTAAGGATAACTACAGAATGAAAAGTTACAAGAACAAAGCCTTAAATCCTCAAGAGA
  TGCGAAGACGAAGAGAAGAAGAAGGAATACAACTTCGAAAACAAAAAAGGGAGGAGCAGTTGTTCAAACG
  CAGAAATGTCTCTTTGCCCAGAAATGATGACTGTATGCTGGAGAGCCCTATCCAGGACCCAGATGTCAGC
  TCCACTGTACCCATTCCAGAGGAAGACATGATCACAGCAGATATGATTCAGATGATTTTCTCTAATAACG
  CTGAACAGCAACTGACTGCAACACAGAAATTTAGAAAACTGCTTTCTAAAGAACCCAATCCACCAATAGA
  TCAAGTTATACAGAAACCAGGAGTTGTACAGAGATTTGTGAAGTTTCTTGAAAGAAATGAAAATTGCACT
  TTACAATTCGAGGCTGCCTGGGCGTTAACTAATATAGCATCTGGAACTTTTCTGCATACCAAGGTAGTGA
  TTGAAACCGGGGCTGTGCCAATTTTTATCAGACTTCTCACTTCAGAACATGAAGATGTACAGGAACAGGC
  GGTTTGGGCACTTGGTAACATTGCTGGCGACAATGCAGAATGCAGGGATTTTGTTTTGAATTGTGAAATA
  CTTCCACCTCTTTTAGAGTTATTAACAAATTCAAACAGACTTACAACCACCAGAAATGCTGTATGGGCCC
  TCTCAAATTTATGTAGAGGCAAAAACCCTCCTCCAAATTTTAGTAAGGTTTCACCTTGCTTAAATGTCTT
  ATCCCGGTTGTTGTTTAGCAGTGATCCAGATGTGTTAGCAGATGTGTGCTGGGCCCTTTCTTATCTCTCT
  GATGGACCCAATGATAAAATTCAAGTAGTCATTGATTCTGGAGTCTGCCGAAGATTGGTGGAACTTTTGA
  TGCACAATGACTATAAAGTTGTATCACCTGCATTAAGAGCAGTTGGTAATATTGTAACTGGTGATGACAT
  TCAAACACAGGTCATTTTGAATTGTTCTGCATTGCCCTGCCTTTTACATTTGCTGGGAAGTCCAAAGGAG
  TCAGTTAGAAAGGAAGCCTGCTGGACCATTTCTAACATCACTGCGGGAAACAGAATGCAGATCCAGGCTG
  TCATAGATGGAAGTATTTTCCCTGTTTTGATTGAGGTCCTCCAGAAAGCAGAATTTCGTACCAGAAAAGA
  AGCAGCGTGGGCTATAACCAATGCCACATCGGGAGGCGCTCCAGAGCAGATAAGGTATTTGGTAACCTTG
  GGCTGCATTAAACCACTTTGTGACCTTTTGACTGTCATGGACTCCAAAATAGTTCAAGTTGCTTTGAATG
  GACTTGAAAATATTTTACGCCTTGGAGAACGAGAATCTAAGCAGAATGGAGTGGGCATCAATCCATACTG
  TGCTCTCATTGAAGAAGCGTACGGCTTGGATAAAATAGAGTTTCTGCAAAGTCATGAAAACCAGGAAATT
  TACCAGAAGGCTTTTGATCTGATTGAACGCTATTTTGGTGTGGAGGAGGATGACCCTAGTCTCGTTCCTC
  AGGTGGATGAGCAGCAGCGGCAGTTTCTCTTTCAGCAGTGCGAGGCACCAGGAGAAGGCTTCCAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212168 representing NM_001025113
  Red=Cloning site Green=Tags(s)
  
  MASPGKDNYRMKSYKNKALNPQEMRRRREEEGIQLRKQKREEQLFKRRNVSLPRNDDCMLESPIQDPDVS
  STVPIPEEDMITADMIQMIFSNNAEQQLTATQKFRKLLSKEPNPPIDQVIQKPGVVQRFVKFLERNENCT
  LQFEAAWALTNIASGTFLHTKVVIETGAVPIFIRLLTSEHEDVQEQAVWALGNIAGDNAECRDFVLNCEI
  LPPLLELLTNSNRLTTTRNAVWALSNLCRGKNPPPNFSKVSPCLNVLSRLLFSSDPDVLADVCWALSYLS
  DGPNDKIQVVIDSGVCRRLVELLMHNDYKVVSPALRAVGNIVTGDDIQTQVILNCSALPCLLHLLGSPKE
  SVRKEACWTISNITAGNRMQIQAVIDGSIFPVLIEVLQKAEFRTRKEAAWAITNATSGGAPEQIRYLVTL
  GCIKPLCDLLTVMDSKIVQVALNGLENILRLGERESKQNGVGINPYCALIEEAYGLDKIEFLQSHENQEI
  YQKAFDLIERYFGVEEDDPSLVPQVDEQQRQFLFQQCEAPGEGFQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025113
• ORF Size: 1608 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025113.1, NP_001020284.1
• RefSeq Size: 1664 bp
• RefSeq ORF: 1611 bp
• Locus ID: 294392
• Cytogenetics: 20q11
• MW: 60.3 kDa