Ptk2 (NM_013081) Rat Tagged ORF Clone

CAT#: RR212236

Ptk2 (Myc-DDK-tagged ORF) - Rat PTK2 protein tyrosine kinase 2 (Ptk2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ptk2
• Synonyms: FAK; FRNK; p125FAK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212236 representing NM_013081
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCTGCTTATCTTGACCCAAACTTGAATCACACACCAAGTTCGAGTACTAAGACTCACCTGGGTA
  CTGGCACGGAACGGTCCCCTGGTGCAATGGAACGAGTATTAAAGGTCTTTCATTATTTTGAAAGCAGTAA
  TGAGCCAACCACCTGGGCCAGTATTATCAGGCATGGAGACGCTACTGACGTCAGGGGAATCATTCAGAAG
  ATAGTGGACAGTCACAAAGTAAAGCACGTGGCCTGCTATGGATTTCGCCTCAGTCACCTGCGATCGGAGG
  AGGTGCACTGGCTGCATGTGGACATGGGTGTCTCCAGTGTGAGGGAGAAATACGAACTCGCCCACCCACC
  AGAGGAGTGGAAATATGAGTTGAGAATTCGTTATTTGCCAAAAGGATTTCTAAACCAGTTTACTGAAGAT
  AAGCCAACATTGAATTTCTTCTATCAACAGGTGAAGAGTGACTACATGCTAGAAATAGCTGATCAAGTAG
  ACCAAGACATAGCTTTGAAGTTGGGTTGTTTGGAAATTAGGCGATCCTACTGGGAGATGAGGGGCAACGC
  CCTAGAGAAGAAGTCCAACTATGAAGTGTTAGAGAAAGATGTTGGTTTAAAGCGATTTTTTCCCAAGAGT
  TTACTGGATTCCGTCAAGGCCAAAACACTAAGAAAGCTGATCCAGCAAACATTCAGGCAGTTTGCCAACC
  TTAATAGAGAAGAAAGCATCCTGAAATTCTTTGAGATTCTCTCTCCAGTGTACAGATTTGACAAAGAGTG
  CTTCAAGTGTGCCCTTGGGTCAAGTTGGATTATTTCTGTGGAACTGGCGATCGGCCCAGAAGAAGGGATC
  AGTTACCTGACAGACAAGGGCTGCAATCCCACACACCTAGCGGACTTCAACCAAGTGCAGACTATCCAGT
  ACTCAAACAGTGAAGACAAAGACAGGAAAGGAATGCTACAACTCAAAATCGCTGGTGCTCCTGAGCCTCT
  GACAGTGACGGCCCCATCCCTAACCATTGCAGAGAACATGGCTGACCTGATCGATGGATACTGCCGGCTG
  GTGAACGGAGCCACGCAGTCCTTCATCATCAGACCCCAGAAAGAAGGTGAACGGGCATTGCCATCAATAC
  CAAAGTTGGCCAACAATGAAAAGCAAGGCATGCGGACACATGCAGTCTCTGTGTCAGAGACAGATGACTA
  TGCCGAGATCATCGATGAGGAAGACACATACACCATGCCCTCAACCAGGGATTATGAGATTCAGAGAGAA
  AGAATAGAACTTGGACGCTGTATTGGAGAAGGCCAGTTTGGAGATGTACATCAAGGCGTGTACCTGAGCC
  CAGAGAATCCAGCTTTGGCTGTTGCAATCAAAACATGTAAAAACTGTACTTCGGACAGCGTGAGAGAGAA
  GTTCCTTCAAGAAGCCTTAACAATGCGCCAGTTTGACCATCCTCACATCGTGAAGCTGATTGGAGTCATT
  ACAGAGAACCCAGTTTGGATAATCATGGAGTTGTGCACACTTGGAGAGCTGAGGTCATTTTTGCAAGTAA
  GGAAATACAGCTTGGATCTAGCATCTTTGATCTTATATGCCTATCAGCTTAGTACAGCACTCGCGTATCT
  GGAGAGCAAAAGATTTGTTCACAGGGACATTGCTGCACGGAATGTTCTGGTGTCTTCAAATGATTGTGTA
  AAATTAGGAGACTTTGGATTATCTCGATATATGGAAGACAGTACTTACTATAAAGCTTCCAAAGGAAAAT
  TACCTATTAAATGGATGGCTCCAGAGTCAATCAATTTTCGACGTTTTACCTCAGCTAGTGACGTGTGGAT
  GTTTGGTGTGTGTATGTGGGAGATACTGATGCATGGCGTGAAGCCTTTTCAAGGAGTGAAGAACAATGAT
  GTGATCGGTCGAATTGAAAATGGGGAAAGATTACCAATGCCTCCAAATTGTCCTCCCACCCTCTACAGCC
  TTATGACGAAATGTTGGGCCTATGACCCCAGCAGACGGCCCAGGTTTACTGAACTAAAAGCTCAGCTCAG
  CACAATCCTGGAGGAAGAGAAGGTGCAGCAAGAAGAACGGATGAGGATGGAATCCAGAAGACAGGCAACA
  GTGTCCTGGGACTCTGGAGGGTCTGATGAAGCACCACCCAAGCCCAGCAGACCTGGTTATCCTAGCCCGA
  GGTCCAGTGAAGGATTTTATCCCAGCCCACAGCACATGGTCCAGACCAATCACTATCAGATCTCTGGCTA
  CCCAGGTTCCCATGGAATCCCAGCCATGGCTGGCAGCATTTACCCAGGTCAGGCATCTCTTCTGGACCAG
  ACAGAATTGTGGAATCACAGACCTCAGGAGATGTCCATGTGGCAGCCCAGTGTGGAGGACTCAGCAGCTT
  TAGATCTTCGAGGGATGGGACAGGTGCTTCCTCCTCACCTGATGGAAGAGCGGCTAATCCGACAGCAGCA
  GGAAATGGAGGAAGACCAGCGCTGGCTGGAAAAGGAAGAGCGATTCCTGAAACCTGATGTGAGGCTCTCT
  CGAGGCAGCATCGACAGGGAGGACGGGAGTTTTCAGGGTCCGACTGGAAACCAACACATCTATCAGCCTG
  TGGGAAAACCAGATCCTGCAGCTCCACCAAAGAAACCACCTCGCCCTGGAGCACCTGGCCACCTAAGCAA
  CTTGTCCAGCATCAGCAGCCCTGCAGAAAGCTACAATGAGGGTGTCAAGCCGTGGAGGCTTCAGCCCCAG
  GAAATCAGCCCCCCTCCCACTGCCAACCTTGACCGATCCAATGACAAGGTATATGAAAATGTGACGGGCC
  TAGTGAAGGCTGTCATTGAGATGTCCAGCAAAATCCAACCAGCTCCTCCAGAAGAGTACGTCCCTATGGT
  GAAGGAAGTTGGCCTGGCCCTGCGGACCTTACTGGCCACTGTGGATGAGACCATTCCCATCCTTCCAGCC
  AGCACCCATCGAGAGATCGAGATGGCACAGAAGCTGCTGAACTCCGACTTGGGTGAGCTCATCAGCAAGA
  TGAAGCTGGCGCAGCAGTATGTCATGACCAGCCTTCAGCAGGAGTACAAGAAGCAGATGCTGACAGCTGC
  TCACGCCCTGGCTGTGGATGCCAAGAATCTACTTGATGTTATTGATCAAGCGAGACTGAAAATGCTAGGG
  CAGACACGGCCACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212236 representing NM_013081
  Red=Cloning site Green=Tags(s)
  
  MAAAYLDPNLNHTPSSSTKTHLGTGTERSPGAMERVLKVFHYFESSNEPTTWASIIRHGDATDVRGIIQK
  IVDSHKVKHVACYGFRLSHLRSEEVHWLHVDMGVSSVREKYELAHPPEEWKYELRIRYLPKGFLNQFTED
  KPTLNFFYQQVKSDYMLEIADQVDQDIALKLGCLEIRRSYWEMRGNALEKKSNYEVLEKDVGLKRFFPKS
  LLDSVKAKTLRKLIQQTFRQFANLNREESILKFFEILSPVYRFDKECFKCALGSSWIISVELAIGPEEGI
  SYLTDKGCNPTHLADFNQVQTIQYSNSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRL
  VNGATQSFIIRPQKEGERALPSIPKLANNEKQGMRTHAVSVSETDDYAEIIDEEDTYTMPSTRDYEIQRE
  RIELGRCIGEGQFGDVHQGVYLSPENPALAVAIKTCKNCTSDSVREKFLQEALTMRQFDHPHIVKLIGVI
  TENPVWIIMELCTLGELRSFLQVRKYSLDLASLILYAYQLSTALAYLESKRFVHRDIAARNVLVSSNDCV
  KLGDFGLSRYMEDSTYYKASKGKLPIKWMAPESINFRRFTSASDVWMFGVCMWEILMHGVKPFQGVKNND
  VIGRIENGERLPMPPNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILEEEKVQQEERMRMESRRQAT
  VSWDSGGSDEAPPKPSRPGYPSPRSSEGFYPSPQHMVQTNHYQISGYPGSHGIPAMAGSIYPGQASLLDQ
  TELWNHRPQEMSMWQPSVEDSAALDLRGMGQVLPPHLMEERLIRQQQEMEEDQRWLEKEERFLKPDVRLS
  RGSIDREDGSFQGPTGNQHIYQPVGKPDPAAPPKKPPRPGAPGHLSNLSSISSPAESYNEGVKPWRLQPQ
  EISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLATVDETIPILPA
  STHREIEMAQKLLNSDLGELISKMKLAQQYVMTSLQQEYKKQMLTAAHALAVDAKNLLDVIDQARLKMLG
  QTRPH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013081
• ORF Size: 3165 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013081.1, NM_013081.2, NP_037213.1
• RefSeq Size: 4559
• RefSeq ORF: 3168
• Locus ID: 25614
• MW: 119.7 kDa
• Gene Summary: tyrosine kinase that is enriched in focal adhesions; may regulate mechanical signal transduction in cardiac myocytes [RGD, Feb 2006]