Tlr4 (NM_019178) Rat Tagged ORF Clone

CAT#: RR212307

Tlr4 (Myc-DDK-tagged ORF) - Rat toll-like receptor 4 (Tlr4), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tlr4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212307 representing NM_019178
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCCTCTCTTGCATCTGGCTGGGACTCTGATCATGGCATTGTTCCTTTCCTGCCTGAGACCAGGAA
  GCTTGAATCCCTGCATAGAGGTACTTCCTAATATTACCTACCAATGCATGGATCAGAATCTCAGCAAAAT
  CCCTCATGACATCCCTTATTCAACCAAGAACCTAGATCTGAGCTTCAACCCCCTGAAGATCTTAAGAAGC
  TATAGCTTCACCAATTTCTCACAACTTCAGTGGCTGGATTTATCCAGGTGTGAAATTGAGACAATTGAAG
  ACAAGGCATGGCATGGCTTAAACCAGCTCTCAACCTTGGTACTGACAGGAAACCCTATCAAGAGTTTTTC
  CCCAGGAAGTTTTTCTGGACTAACAAATTTAGAGAATCTGGTGGCTGTGGAGACAAAAATGACCTCTCTA
  GAGGGTTTCCATATTGGACAGCTTATATCCTTAAAGAAACTAAATGTGGCTCATAATCTTATACATTCCT
  TTAAGTTGCCTGAATATTTTTCTAATCTGACAAACCTAGAACATGTGGATCTTTCTTATAACTATATTCA
  AACTATTTCTGTCAAAGACTTACAGTTTCTACGTGAAAATCCCCAAGTCAATCTCTCTTTAGACCTGTCT
  TTAAACCCAATTGACTCCATTCAAGCCCAAGCCTTTCAGGGAATTAGGCTCCATGAATTGACTCTAAGAA
  GTAATTTTAATAGCTCAAATGTACTGAAAATGTGCCTTCAAAACATGACTGGTTTACATGTCCATCGGTT
  GATCTTGGGAGAATTTAAAAATGAAAGGAATCTGGAAAGTTTTGACCGTTCTGTCATGGAAGGACTATGC
  AATGTGAGCATTGATGAGTTCAGGTTAACATATATAAATCATTTTTCAGATGATATTTATAATCTCAATT
  GCTTGGCAAATATTTCTGCAATGTCTTTCACAGGTGTACATATAAAACACATAGCAGATGTTCCTAGGCA
  TTTCAAATGGCAATCCTTATCAATCATTAGATGTCATCTTAAGCCTTTTCCAAAGCTGAGTCTACCTTTT
  CTTAAAAGTTGGACTTTAACTACCAACAGAGAGGATATCAGCTTTGGTCAGTTGGCTCTGCCAAGTCTCA
  GATATCTAGATCTTAGTAGAAATGCCATGAGCTTTAGAGGTTGCTGTTCTTATTCTGATTTTGGAACAAA
  CAACCTGAAGTACTTAGACCTCAGCTTCAATGGTGTCATCCTGATGAGTGCCAACTTCATGGGTCTAGAA
  GAGCTGGAATACCTGGACTTTCAGCACTCCACTTTAAAAAAGGTCACAGAATTCTCAGTGTTCTTATCTC
  TTGAAAAACTTCTTTACCTTGACATCTCTTACACTAATACCAAAATTGACTTTGATGGCATATTTCTTGG
  CTTGATCAGTCTCAACACTTTAAAAATGGCTGGCAATTCTTTCAAAGACAACACCCTTTCAAATGTCTTT
  ACAAACACAACAAACTTAACATTCCTGGATCTTTCTAAATGCCAACTGGAACAGATATCTAGGGGGGTAT
  TTGACACACTCTACAGACTCCAGTTATTAAACATGAGTCACAACAACCTACTGTTTCTGGATCCATCCCA
  TTATAAACAGCTGTACTCCCTCAGGACTCTTGATTGCAGTTTCAATCGCATAGAGACATCCAAAGGAATA
  CTGCAACATTTTCCAAAGAGTCTAGCCGTCTTCAATCTGACTAATAATTCTGTTGCTTGTATATGTGAAT
  ATCAGAATTTCTTGCAGTGGGTCAAGGACCAGAAAATGTTCTTGGTGAATGTTGAACAAATGAAATGTGC
  ATCACCTATAGACATGAAGGCCTCCCTGGTGTTGGATTTTACGAATTCCACCTGTTATATATACAAGACT
  ATCATCAGTGTATCGGTGGTCAGTGTGCTTGTGGTAGCCACTGTAGCATTTCTGATATACCACTTCTATT
  TTCACCTGATACTTATTGCTGGCTGTAAAAAGTACAGCAGAGGAGAAAGCATCTATGATGCATTTGTGAT
  CTACTCGAGCCAGAATGAGGACTGGGTGAGAAACGAGCTGGTAAAGAATTTAGAAGAAGGAGTGCCCCGC
  TTTCAGCTTTGCCTTCATTACAGGGACTTTATTCCTGGTGTAGCCATTGCTGCCAACATCATCCAGGAAG
  GCTTCCACAAGAGCCGGAAAGTTATTGTGGTGGTGTCTAGACACTTTATCCAGAGCCGTTGGTGTATCTT
  TGAATATGAGATTGCTCAGACATGGCAGTTTCTGAGTAGCCGCTCTGGCATCATCTTCATTGTCCTTGAG
  AAAGTGGAGAAGTCCTTGCTGAGGCAGCAGGTCGAATTGTATCGCCTTCTTAGCAGAAACACCTACCTCG
  AGTGGGAGGACAATGCTCTGGGGAGGCACATCTTCTGGAGAAGACTCAAAAAAGCCCTGTTGGATGGAAA
  AGCCTTGAATCCAGATGAAACATCAGAGGAAGAACAAGAAGCAACAACTTTGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212307 representing NM_019178
  Red=Cloning site Green=Tags(s)
  
  MMPLLHLAGTLIMALFLSCLRPGSLNPCIEVLPNITYQCMDQNLSKIPHDIPYSTKNLDLSFNPLKILRS
  YSFTNFSQLQWLDLSRCEIETIEDKAWHGLNQLSTLVLTGNPIKSFSPGSFSGLTNLENLVAVETKMTSL
  EGFHIGQLISLKKLNVAHNLIHSFKLPEYFSNLTNLEHVDLSYNYIQTISVKDLQFLRENPQVNLSLDLS
  LNPIDSIQAQAFQGIRLHELTLRSNFNSSNVLKMCLQNMTGLHVHRLILGEFKNERNLESFDRSVMEGLC
  NVSIDEFRLTYINHFSDDIYNLNCLANISAMSFTGVHIKHIADVPRHFKWQSLSIIRCHLKPFPKLSLPF
  LKSWTLTTNREDISFGQLALPSLRYLDLSRNAMSFRGCCSYSDFGTNNLKYLDLSFNGVILMSANFMGLE
  ELEYLDFQHSTLKKVTEFSVFLSLEKLLYLDISYTNTKIDFDGIFLGLISLNTLKMAGNSFKDNTLSNVF
  TNTTNLTFLDLSKCQLEQISRGVFDTLYRLQLLNMSHNNLLFLDPSHYKQLYSLRTLDCSFNRIETSKGI
  LQHFPKSLAVFNLTNNSVACICEYQNFLQWVKDQKMFLVNVEQMKCASPIDMKASLVLDFTNSTCYIYKT
  IISVSVVSVLVVATVAFLIYHFYFHLILIAGCKKYSRGESIYDAFVIYSSQNEDWVRNELVKNLEEGVPR
  FQLCLHYRDFIPGVAIAANIIQEGFHKSRKVIVVVSRHFIQSRWCIFEYEIAQTWQFLSSRSGIIFIVLE
  KVEKSLLRQQVELYRLLSRNTYLEWEDNALGRHIFWRRLKKALLDGKALNPDETSEEEQEATTLT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019178
• ORF Size: 2505 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_019178.1, NP_062051.1
• RefSeq Size: 3373
• RefSeq ORF: 2508
• Locus ID: 29260
• MW: 96.5 kDa
• Gene Summary: receptor that functions as the major upstream sensor for hemorrhagic shock and lipopolysaccharide [RGD, Feb 2006]