Clint1 (NM_001002022) Rat Tagged ORF Clone

CAT#: RR212338

Clint1 (Myc-DDK-tagged ORF) - Rat enthoprotin (Enth), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Clint1
• Synonyms: Clint; Enth; Epn4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212338 representing NM_001002022
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAACATGTGGAAGGTGCGGGAGCTGGTGGACAAAGCCACCAATGTGGTTATGAATTATTCAGAGA
  TCGAGTCCAAAGTTCGAGAAGCAACGAATGATGATCCTTGGGGACCTTCTGGGCAGCTCATGGGAGAGAT
  TGCCAAGGCTACTTTTATGTATGAACAGTTCCCAGAACTTATGAACATGCTTTGGTCCCGAATGCTAAAA
  GACAACAAAAAGAATTGGAGAAGAGTTTATAAGTCGTTGCTGCTCCTAGCTTACCTCATAAGAAATGGAT
  CGGAGCGAGTTGTTACAAGCGCCAGAGAACACATTTATGATTTGCGATCCCTGGAAAATTACCACTTTGT
  AGATGAGCATGGCAAGGATCAAGGTATAAACATCCGACAGAAAGTGAAGGAGCTGGTTGAGTTTGCACAG
  GATGATGACAGACTTCGAGAGGAGAGGAAGAAAGCAAAGAAGAACAAAGACAAGTATGTCGGGGTCTCCT
  CAGACAGCGTTGGCGGATTCCGATACAATGAACGGTATGACCCTGAGCCCAAGTCCAAATGGGACGAAGA
  GTGGGATAAGAACAAGAGTGCGTTTCCATTCAGTGACAAGCTAGGGGAGCTGAGCGACAAGATCGGGAGC
  ACAATCGACGACACCATCAGCAAGTTCCGGAGGAAAGACCGGGAGGACTCTCCGGAAAGGTGCAGTGACA
  GCGATGAGGAAAAGAAAGCAAGGAGAGGCAGGTCTCCCAAAGGTGAATTCAAAGATGAAGAGGAGACGGT
  GACGACGAAGCACATCCACATCACACAGGCCACAGAGACCACCACCACCCGGCACAAGCGCACAGCAAAC
  CCTTCCAAAACCATCGATCTTGGAGCAGCAGCACATTACACAGGGGACAAAGCCAGCCCAGATCAAAACG
  CTTCAACCCACACGCCTCAGTCATCAGCGAAGCCATCCGTGCCGAGCAGCAAGTCATCAGGGGACCTCGT
  TGACCTGTTTGATGGCAGTGGCCAGACAACAGGAGGAGCAACTGACTTATTCGGGGGATTTGCCGACTTT
  GGCTCTGCTGCTGCATCGGGCAGTTTCCCTTCCCAAGCAACAAGTGGGAATGGAGACTTTGGTGACTGGA
  GTGCCTTCAACCAAGCCCCATCAGGCCCTGTTGCTTCTGGTGGTGAGCTCTTCGGCAGTGCCCCACAGCC
  TGCTGTAGAACTCATCAGTGGTTCACAGCCAGCTTTAGGCCCACCTCCTGCTGCCTCAAACTCTTCAGAT
  CTCTTTGATCTTATGGGCTCATCCCAGGCAACCATGACGTCTTCCCAGAGTATGAATTTCTCTCTAATGA
  GCACTAACACTGTGGGACTTGGTTTGCCCATGTCAAGATCACAGGTAAGATGTCTGTTTCCCTTGGGAAT
  TGTTACTTACACCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212338 representing NM_001002022
  Red=Cloning site Green=Tags(s)
  
  MLNMWKVRELVDKATNVVMNYSEIESKVREATNDDPWGPSGQLMGEIAKATFMYEQFPELMNMLWSRMLK
  DNKKNWRRVYKSLLLLAYLIRNGSERVVTSAREHIYDLRSLENYHFVDEHGKDQGINIRQKVKELVEFAQ
  DDDRLREERKKAKKNKDKYVGVSSDSVGGFRYNERYDPEPKSKWDEEWDKNKSAFPFSDKLGELSDKIGS
  TIDDTISKFRRKDREDSPERCSDSDEEKKARRGRSPKGEFKDEEETVTTKHIHITQATETTTTRHKRTAN
  PSKTIDLGAAAHYTGDKASPDQNASTHTPQSSAKPSVPSSKSSGDLVDLFDGSGQTTGGATDLFGGFADF
  GSAAASGSFPSQATSGNGDFGDWSAFNQAPSGPVASGGELFGSAPQPAVELISGSQPALGPPPAASNSSD
  LFDLMGSSQATMTSSQSMNFSLMSTNTVGLGLPMSRSQVRCLFPLGIVTYTY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001002022
• ORF Size: 1416 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002022.1, NP_001002022.1
• RefSeq Size: 1841 bp
• RefSeq ORF: 1419 bp
• Locus ID: 360515
• Cytogenetics: 10q21
• MW: 52.1 kDa
• Gene Summary: stimulates clathrin assembly; may play a role in clathrin mediated budding and vesicular trafficking [RGD, Feb 2006]