Mecp2 (NM_022673) Rat Tagged ORF Clone

CAT#: RR212432

Mecp2 (Myc-DDK-tagged ORF) - Rat methyl CpG binding protein 2 (Mecp2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mecp2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212432 representing NM_022673
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAGCTGGGATGTTAGGGCTCAGGGAGGAAAAGTCAGAAGACCAGGATCTCCAGGGCCTCAAAGAGA
  AACCCCTGAAGTTTAAGAAGGTGAAGAAAGACAAGAAGGAAGACAAAGAGGGCAAACATGAACCACTACA
  GCCTTCAGCCCACCATTCTGCAGAGCCAGCAGAGGCAGGCAAAGCAGAGACATCAGAAAGCTCAGGCTCT
  GCCCCAGCAGTACCAGAAGCCTCTGCTTCTCCCAAACAGCGACGTTCCATCATTCGTGACCGGGGACCTA
  TGTATGATGACCCCACCTTGCCTGAAGGTTGGACGCGAAAGCTTAAACAGAGGAAGTCTGGTCGCTCTGC
  TGGAAAGTATGATGTATATTTGATCAATCCCCAGGGAAAAGCCTTTCGCTCTAAAGTAGAATTGATTGCA
  TATTTTGAAAAGGTGGGAGACACCTCCTTGGACCCTAATGATTTTGACTTCACTGTAACTGGGAGAGGGA
  GCCCTTCCAGGAGAGAACAGAAACCACCTAAGAAGCCCAAATCTCCCAAAGCTCCAGGAACTGGCAGGGG
  TCGGGGACGCCCGAAAGGGAGCGGCACTGGGAGACCAAAGGCAGCAGCATCAGAAGGTGTTCAAGTGAAA
  AGGGTCCTGGAGAAGAGCCCTGGGAAACTTCTCGTCAAGATGCCTTTCCAAGCATCACCTGGGGGTAAGG
  GTGAGGGAGGTGGGGCTACCACATCTGCGCAGGTCATGGTGATCAAACGCCCTGGCAGAAAGCGAAAAGC
  TGAAGCTGACCCCCAGGCCATTCCTAAGAAACGGGGTAGAAAGCCTGGGAGTGTGGTGGCAGCTGCTGCT
  GCAGAGGCCAAAAAGAAAGCTGTGAAGGAATCTTCTATACGGTCTGTGCAGGAGACTGTGCTCCCCATCA
  AGAAGCGCAAGACCCGGGAAACCGTCAGCATTGAGGTCAAGGAGGTGGTGAAGCCCCTGCTGGTGTCTAC
  ACTTGGTGAGAAGAGTGGAAAGGGACTGAAGACATGCAAGAGCCCTGGGCGTAAAAGCAAGGAGAGCAGC
  CCCAAGGGGCGCAGCAGCAGTGCCTCCTCACCACCTAAGAAGGAGCACCATCATCACCACCATCACGCAG
  AGTCCCCAAAGGCCCCCATGCCATTGCTTCCACCTCCACCCCCACCTGAGCCTCAGAGCTCTGAGGACCC
  CATCAGCCCCCCTGAGCCTCAGGACTTGAGCAGCAGCATCTGCAAAGAGGAGAAGATGCCCCGAGCAGGC
  TCACTGGAAAGCGATGGCTGCCCCAAGGAGCCAGCTAAGACTCAGCCCATGGTTGCTGCCGCCGCCACCA
  CCACCACCACCACCACCACCACAGTTGCAGAAAAGTACAAACACCGAGGGGAGGGAGAGCGCAAAGACAT
  TGTTTCATCCTCCATGCCGAGGCCAAACAGAGAGGAGCCTGTGGACAGCCGGACGCCCGTGACCGAGAGA
  GTTAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212432 representing NM_022673
  Red=Cloning site Green=Tags(s)
  
  MVAGMLGLREEKSEDQDLQGLKEKPLKFKKVKKDKKEDKEGKHEPLQPSAHHSAEPAEAGKAETSESSGS
  APAVPEASASPKQRRSIIRDRGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIA
  YFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTGRPKAAASEGVQVK
  RVLEKSPGKLLVKMPFQASPGGKGEGGGATTSAQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAA
  AEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESS
  PKGRSSSASSPPKKEHHHHHHHAESPKAPMPLLPPPPPPEPQSSEDPISPPEPQDLSSSICKEEKMPRAG
  SLESDGCPKEPAKTQPMVAAAATTTTTTTTTVAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTER
  VS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022673
• ORF Size: 1476 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022673.1, NM_022673.2, NP_073164.2
• RefSeq Size: 1711
• RefSeq ORF: 1479
• Locus ID: 29386
• MW: 53.5 kDa
• Gene Summary: methyl-CpG-binding protein; binds to DNA that contains a single methyl-CpG pair [RGD, Feb 2006]