Man2a2 (NM_001107527) Rat Tagged ORF Clone

CAT#: RR212469

Man2a2 (Myc-DDK-tagged ORF) - Rat mannosidase 2, alpha 2 (Man2a2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Man2a2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212469 representing NM_001107527
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTGAAAAAGCAGGTGACAGTGTGCGGGGCTGCTATCTTCTGTGTGGCCGTCTTTTCCCTGTACC
  TAATGCTGGACCGAGTGCAGCATGATCCTGCCAGACACCAGAATGGTGGGAACTTCCCCAGGAGCCAAAT
  TTCTGTGCTACAGAACCGGATCGAACAGCTGGAACAGCTGCTGGAAGAAAACCATGAGATCATAAGCCAT
  ATCAAGGACTCTGTGCTGGAACTGACAGCCAATGCGGAGGGCCCACCAGCCCTGCTGCCCTACCACACAG
  CCAACGGCTCCTGGGCTGTGCTCCCCGAGCCCCGGCCCAGCTTCTTCTCTGTATCCCCTGAGGACTGCCA
  GTTTGCTTTGGGGGGCCGGGGTCAGAAGCCAGAGCTACAGATGTTAACTGTGTCTGAGGATTTGCCGTTT
  GACAATGTGGAGGGCGGCGTGTGGAGGCAAGGCTTCGACATCTCCTACAGCCCAAATGACTGGGATGCTG
  AAGACCTGCAGGTGTTTGTGGTGCCTCACTCCCACAATGATCCAGGCTGGATCAAGACTTTTGACAAGTA
  CTACACGGAACAAACCCAACACATCCTCAACAGCATGGTGTCCAAGCTGCAGGAAGATCCCCGACGGCGC
  TTTCTCTGGGCAGAAGTCTCCTTCTTCGCCAAGTGGTGGGACAACATCAGTGCCCAGAAAAGGGCAGCAG
  TTCGAAGGCTGGTGGGAAATGGGCAGCTGGAAATTGCAACGGGTGGATGGGTGATGCCAGATGAGGCCAA
  CTCCCATTACTTTGCCCTGGTCGACCAGCTCATCGAGGGACACCAGTGGCTGGAGAGAAACCTGGGTGCA
  ACTCCTCGCTCGGGCTGGGCAGTGGACCCCTTTGGACACAGCTCCACCATGCCTTACCTGCTGCGCCGTG
  CCAACCTGACCAGCATGCTAATTCAGAGGGTGCATTACGCCATCAAGAAGCACTTTGCGGCCACTCACAG
  CCTGGAGTTCATGTGGAGGCAGACATGGGATTCAGACTCCAGCACAGACATCTTCTGCCACATGATGCCC
  TTCTACAGCTACGACGTCCCACACACCTGTGGCCCTGATCCCAAGATCTGCTGCCAGTTTGATTTCAAAC
  GTCTGCCGGGTGGGAGAATCAATTGTCCTTGGAAGGTGCCGCCGCGGGCTATCACAGAGGCCAACGTGGC
  AGACAGGGCAGCCCTGCTCCTGGACCAGTACCGGAAGAAGTCCCGGCTGTTTCGAAGCAGTGTCCTCCTT
  GTGCCGCTGGGTGATGACTTCCGATATGACAAGCCCCAGGAATGGGATGCCCAGTTCTTCAACTATCAAC
  GGCTCTTTGACTTCCTCAACAGCAAGCCGGAGTTCCACGTACAGGCACAGTTTGGGACCCTCTCTGAGTA
  TTTTGATGCCCTGTATAAGAGGACAGGAGTGGAGCCTGGTGCCCGGCCTCCAGGGTTTCCTGTGCTGAGT
  GGGGACTTCTTCTCCTATGCTGACCGGGAGGACCACTACTGGACAGGCTATTACACTTCCCGGCCTTTCT
  ATAAGAGCTTGGACCGCGTGCTAGAAACTCACCTTCGTGGGGCAGAGGTTCTATACAGCCTGGCTTTGGC
  GCATGCCCGCCGTTCTGGACTGACTGGCCAGTATCCGCTGTCTGATTACGCTGTCCTGACGGAAGCTCGT
  CGTACACTGGGCCTCTTCCAGCACCACGATGCCATCACCGGAACTGCCAAGGAGGCAGTTGTAGTAGACT
  ATGGGGTCAGGTTGCTGCGTTCCCTGGTCAGCCTAAAGCAGGTCATCATCAATGCTGCCCACTACCTGGT
  GCTGGGGGACAAGGAGACCTACAGCTTTGACCCTAGGGCACCCTTCCTCCAAATGGATGACAGTCGAGTA
  AGTCACGATGCCCTGCCTGAGCGCACGGTGATCCGTCTGGACTCCTCACCCAGGTTTGTGGTGGTCTTTA
  ACCCACTGGAACAGGAGCGGCTCAGTGTGGTGTCCCTGCTGGTCAACTCACCCCGAGTGCGAGTGCTTTC
  AGAGGAGGGTCAGCCCTTGTCTGTGCAGATCAGTGTGCAGTGGAGCTCCGCCACCAACATGGTCCCCGAT
  GTCTACCAGGTGTCAGTGCCTGTCCGCCTGCCAGCCCTGGGCCTGGGTGTGCTGCAGCTGCAGCCAGATC
  TCGATGGACCCTACACACTGCAGTCTTCGGTGCATGTCTACCTGAACGGCGTGAAACTGTCTGTCAGCAG
  GCAAACAACATTCCCTCTCCGTGTTGTGGACTCGGGCACCAGTGACTTCGCCATCAGCAATCGATACATG
  CAGGTCTGGTTCTCCGGCCTTACTGGGCTTCTCAAGAGCGTCCGACGTGTGGACGAAGAGCAGGAACAGC
  AGGTGGACATGAAGCTCTTCGTCTATGGAACCCGCACATCCAAGGATAAGAGTGGTGCCTACCTCTTCCT
  GCCTGATAACGAGGCTAAGCCCTATGTCCCTAAGAAACCTCCTGTGCTGCGTGTCACCGAAGGCCCTTTC
  TTCTCAGAGGTGGCTGCGTATTATGAGCACTTTCACCAAGTGATTCGACTTTACAACCTGCCAGGGGTAG
  AGGGGCTGTCTCTGGACGTGTCGTTCCAGGTGGACATCAGGGACTACGTGAACAAGGAGCTAGCCCTGCG
  CATCCACACAGACATCGACAGCCAGGGCACTTTCTTCACAGACCTCAATGGCTTTCAGGTACAGCCCCGG
  AAGTATCTGAAGAAGTTGCCCCTGCAGGCTAATTTCTACCCTATGCCAGTCATGGCCTACATCCAGGATT
  CCCAGAGGCGCCTCACGCTGCACACTGCTCAGGCTCTGGGTGTCTCCAGCCTCGGCAATGGCCAGCTGGA
  GGTGATCTTGGACCGAAGGCTAATGCAGGATGACAACCGGGGACTAGGCCAAGGGCTCAAAGACAACAAG
  ATCACCTGCAACCATTTCCGCCTCCTGTTAGAACGTCGAACCCTGATGAGCCCTGAGGTCCAACAGGAGC
  GCTCTACAAGCTACCCGTCCCTCCTCAGCCACATGACTTCCATGTACCTCAACACACCTCCTCTGGTCTT
  ACCGGTGGCCAAGAGGGAGAGCACCAGCCCCACTCTGCACTCTTTCCACCCTCTGGCTTCTCCGTTGCCC
  TGCGATTTCCATCTGCTCAATCTGCGCATGCTCCCCGCCGAGGTGAGTGTCCCGGTCCGTGCCAATCCTC
  ACCATCAGGCTGAGGACACCTTGCCCGCGGCTGATGCTGCTCTCATCCTACACCGCAAGGGTTTTGACTG
  TGGCCTTGAAGCCAAGAACCTGGGCTTCAACTGTACCACAAGCCAAGGCAAGCTGGCCCTGGGGAGCCTC
  TTCCATGGCCTGGATGTGCTATTCCTGCAGCCCACCTCTTTGACTTTGCTATACCCTCTGGCCTCGCCCT
  CCAACAGCACTGACATCTCTCTGGAGCCCATGGAGATCAGCACCTTCCGCCTGCGCTTGGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212469 representing NM_001107527
  Red=Cloning site Green=Tags(s)
  
  MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISH
  IKDSVLELTANAEGPPALLPYHTANGSWAVLPEPRPSFFSVSPEDCQFALGGRGQKPELQMLTVSEDLPF
  DNVEGGVWRQGFDISYSPNDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHILNSMVSKLQEDPRRR
  FLWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALVDQLIEGHQWLERNLGA
  TPRSGWAVDPFGHSSTMPYLLRRANLTSMLIQRVHYAIKKHFAATHSLEFMWRQTWDSDSSTDIFCHMMP
  FYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPRAITEANVADRAALLLDQYRKKSRLFRSSVLL
  VPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPEFHVQAQFGTLSEYFDALYKRTGVEPGARPPGFPVLS
  GDFFSYADREDHYWTGYYTSRPFYKSLDRVLETHLRGAEVLYSLALAHARRSGLTGQYPLSDYAVLTEAR
  RTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIINAAHYLVLGDKETYSFDPRAPFLQMDDSRV
  SHDALPERTVIRLDSSPRFVVVFNPLEQERLSVVSLLVNSPRVRVLSEEGQPLSVQISVQWSSATNMVPD
  VYQVSVPVRLPALGLGVLQLQPDLDGPYTLQSSVHVYLNGVKLSVSRQTTFPLRVVDSGTSDFAISNRYM
  QVWFSGLTGLLKSVRRVDEEQEQQVDMKLFVYGTRTSKDKSGAYLFLPDNEAKPYVPKKPPVLRVTEGPF
  FSEVAAYYEHFHQVIRLYNLPGVEGLSLDVSFQVDIRDYVNKELALRIHTDIDSQGTFFTDLNGFQVQPR
  KYLKKLPLQANFYPMPVMAYIQDSQRRLTLHTAQALGVSSLGNGQLEVILDRRLMQDDNRGLGQGLKDNK
  ITCNHFRLLLERRTLMSPEVQQERSTSYPSLLSHMTSMYLNTPPLVLPVAKRESTSPTLHSFHPLASPLP
  CDFHLLNLRMLPAEVSVPVRANPHHQAEDTLPAADAALILHRKGFDCGLEAKNLGFNCTTSQGKLALGSL
  FHGLDVLFLQPTSLTLLYPLASPSNSTDISLEPMEISTFRLRLG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107527
• ORF Size: 3492 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001107527.1, NP_001100997.1
• RefSeq Size: 4200
• RefSeq ORF: 3495
• Locus ID: 308757
• MW: 132 kDa