Dgkz (NM_031143) Rat Tagged ORF Clone

CAT#: RR212478

Dgkz (Myc-DDK-tagged ORF) - Rat diacylglycerol kinase zeta (Dgkz), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dgkz
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212478 representing NM_031143
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGCGGGACCCCAGCCCCGAGGCCCGGAGCAGCGACTCGGAGTCGGCCTCCGCCTCGTCCAGCG
  GCTCCGAGCGCGACGCGGATCCGGAGCCGGACAAGGCTCCGCGGCGCTTAACCAAGAGGCGCTTCCCGGG
  GCTGCGGCTCTTTGGGCACAGGAAAGCCATCACCAAGTCGGGCCTCCAGCACCTGGCACCCCCTCCTCCC
  ACGCCTGGGGCCCCGTGCGGTGAATCTGAGCGGCAGATCCGGAGTACTGTGGACTGGAGTGAGTCAGCAG
  CGTATGGGGAGCACATCTGGTTTGAGACCAACGTGTCCGGAGATTTCTGCTATGTCGGGGAGCAGTACTG
  TGTAGCTAAGATGCTGCCGAAGTCAGCGCCCAGAAGAAAGTGTGCAGCCTGTAAGATCGTGGTGCATACC
  CCTTGCATCGGGCAGCTGGAAAAGATCAATTTCCGCTGTAAGCCATCCTTCCGTGAATCAGGCTCCAGGA
  ATGTCCGTGAGCCAACCTTCGTAAGACACCACTGGGTCCACAGGCGACGCCAGGATGGCAAGTGTCGGCA
  CTGTGGGAAGGGCTTCCAGCAGAAGTTCACCTTCCACAGCAAAGAGATCGTAGCTATCAGCTGTTCCTGG
  TGCAAACAGGCGTACCACAGCAAGGTGTCCTGCTTCATGCTGCAGCAGATTGAGGAGCCCTGTTCCCTAG
  GGGTGCATGCAGCCGTGGTCATCCCACCCACCTGGATCCTACGGGCCCGGAGGCCCCAGAACACCCTCAA
  GGCCAGCAAGAAGAAAAAGAGAGCATCCTTCAAAAGGAGATCCAGTAAGAAAGGACCTGAGGAAGGCCGC
  TGGAGACCCTTCATCATCAGGCCCACCCCGTCCCCCCTCATGAAGCCCCTGCTAGTGTTTGTAAACCCCA
  AGAGTGGGGGTAACCAGGGCGCTAAGATCATCCAGTCTTTTCTGTGGTATCTGAACCCCCGGCAAGTCTT
  CGATCTGAGCCAGGGAGGACCCAGGGAAGCGCTGGAAATGTACCGCAAAGTCCATAATTTGAGGATTCTG
  GCTTGCGGGGGTGATGGCACGGTTGGCTGGATTCTTTCCACCCTAGACCAGCTGCGCTTAAAACCACCAC
  CTCCTGTGGCCATCCTGCCCCTGGGCACTGGCAATGACCTGGCCCGAACTCTCAACTGGGGTGGGGGTTA
  CACAGATGAGCCAGTGTCAAAGATCCTTTCCCATGTAGAGGAGGGGAACGTGGTACAGCTGGACCGTTGG
  GACCTCCGAGCAGAGCCCAACCCTGAGGCAGGGCCTGAGGAGCGAGATGACGGAGCCACTGACCGGCTGC
  CCCTGGATGTCTTCAACAACTACTTCAGTCTGGGCTTTGATGCCCACGTCACCCTGGAGTTTCATGAGTC
  TCGAGAAGCCAACCCAGAGAAGTTCAACAGCCGCTTTCGGAATAAGATGTTCTATGCTGGGACAGCCTTC
  TCTGACTTCCTGATGGGCAGCTCCAAGGACTTAGCCAAGCACATCCGAGTAGTGTGTGACGGAATGGACC
  TAACCCCCAAGATTCAGGACCTGAAACCGCAGTGCATAGTCTTTCTGAATATCCCCAGGTACTGTGCAGG
  CACCATGCCCTGGGGCCACCCTGGGGAGCACCATGACTTCGAGCCCCAGAGGCACGATGATGGCTACCTC
  GAGGTCATCGGCTTCACCATGACATCCTTGGCCGCATTGCAGGTGGGTGGACACGGCGAGCGGCTGACCC
  AGTGCCGAGAAGTGCTGCTCACCACTGCCAAGGCCATCCCTGTGCAGGTGGATGGTGAACCCTGCAAGCT
  TGCAGCATCGCGGATTCGAATTGCCCTGCGCAACCAGGCCACTATGGTGCAGAAGGCCAAGCGCCGGAGT
  ACTGCCCCACTGCACAGCGATCAGCAGCCAGTTCCTGAGCAGCTGAGGATCCAAGTGAGCAGGGTCAGCA
  TGCACGACTACGAGGCTCTCCATTATGACAAGGAGCAGCTCAAGGAGGCCTCTGTGCCTCTGGGTACTGT
  GGTGGTCCCCGGAGACAGTGACCTAGAGCTCTGCCGAGCCCACATTGAGAGACTCCAGCAGGAGCCCGAT
  GGGGCTGGAGCCAAGTCCCCCATGTGCCACCCACTGTCCTCCAAGTGGTGTTTCCTGGACGCCACCACTG
  CCAGCCGCTTCTACAGGATCGACAGGGCCCAGGAGCACCTCAACTATGTGACGGAGATTGCCCAGGATGA
  GATTTATATCCTAGACCCAGAGCTGCTGGGTGCATCAGCCCGACCTGACCTCCCTACCCCTACCTCCCCG
  CTCCCTGCCTCCCCCTGCTCCCCTACACCCGGGTCACTGCAGGGGGATGCCGCACTGCCCCAAGGTGAAG
  AGCTGATTGAAGCTGCCAAGAGGAATGACTTCTGCAAGCTCCAGGAACTACACCGAGCAGGAGGTGACCT
  CATGCACCGCGACCATCAGAGCCGCACACTCCTGCACCACGCAGTCAGCACTGGCAGTAAGGAAGTGGTC
  CGCTATCTGCTCGATCATGCACCACCAGAGATCCTCGATGCTGTGGAGGAGAACGGGGAGACCTGTCTAC
  ACCAGGCAGCCGCCCTAGGTCAGCGCACCATCTGCCACTACATTGTGGAAGCCGGGGCCTCCCTCATGAA
  GACAGATCAGCAGGGCGACACTCCCAGGCAGCGAGCTGAGAAGGCTCAAGACACAGAGCTAGCTGCATAC
  CTGGAGAACAGACAACATTACCAGATGATCCAACGTGAGGACCAGGAGACAGCTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212478 representing NM_031143
  Red=Cloning site Green=Tags(s)
  
  MEPRDPSPEARSSDSESASASSSGSERDADPEPDKAPRRLTKRRFPGLRLFGHRKAITKSGLQHLAPPPP
  TPGAPCGESERQIRSTVDWSESAAYGEHIWFETNVSGDFCYVGEQYCVAKMLPKSAPRRKCAACKIVVHT
  PCIGQLEKINFRCKPSFRESGSRNVREPTFVRHHWVHRRRQDGKCRHCGKGFQQKFTFHSKEIVAISCSW
  CKQAYHSKVSCFMLQQIEEPCSLGVHAAVVIPPTWILRARRPQNTLKASKKKKRASFKRRSSKKGPEEGR
  WRPFIIRPTPSPLMKPLLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPREALEMYRKVHNLRIL
  ACGGDGTVGWILSTLDQLRLKPPPPVAILPLGTGNDLARTLNWGGGYTDEPVSKILSHVEEGNVVQLDRW
  DLRAEPNPEAGPEERDDGATDRLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAF
  SDFLMGSSKDLAKHIRVVCDGMDLTPKIQDLKPQCIVFLNIPRYCAGTMPWGHPGEHHDFEPQRHDDGYL
  EVIGFTMTSLAALQVGGHGERLTQCREVLLTTAKAIPVQVDGEPCKLAASRIRIALRNQATMVQKAKRRS
  TAPLHSDQQPVPEQLRIQVSRVSMHDYEALHYDKEQLKEASVPLGTVVVPGDSDLELCRAHIERLQQEPD
  GAGAKSPMCHPLSSKWCFLDATTASRFYRIDRAQEHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSP
  LPASPCSPTPGSLQGDAALPQGEELIEAAKRNDFCKLQELHRAGGDLMHRDHQSRTLLHHAVSTGSKEVV
  RYLLDHAPPEILDAVEENGETCLHQAAALGQRTICHYIVEAGASLMKTDQQGDTPRQRAEKAQDTELAAY
  LENRQHYQMIQREDQETAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031143
• ORF Size: 2787 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031143.1, NP_112405.1
• RefSeq Size: 3560
• RefSeq ORF: 2790
• Locus ID: 81821
• MW: 104 kDa
• Gene Summary: catalyzes the conversion of diacylglycerol to phosphatidic acid [RGD, Feb 2006]