Ctsb (NM_022597) Rat Tagged ORF Clone

CAT#: RR212662

Ctsb (Myc-DDK-tagged ORF) - Rat cathepsin B (Ctsb), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ctsb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212662 representing NM_022597
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGTGGTCCTTGATCCCTCTCTCTTGCCTGCTGGCACTGACCAGTGCCCATGACAAGCCTTCCTTTC
  ACCCACTGTCGGACGACATGATTAACTATATCAACAAACAGAATACAACATGGCAGGCTGGACGCAACTT
  CTACAATGTTGACATAAGCTATCTGAAGAAGCTGTGTGGAACTGTCCTGGGTGGACCCAAGCTGCCGGAA
  AGGGTTGGGTTCAGCGAGGACATAAATCTACCTGAATCCTTTGATGCACGGGAACAGTGGTCCAATTGCC
  CGACCATCGCACAGATCAGAGACCAGGGGTCCTGTGGCTCTTGTTGGGCATTTGGGGCAGTGGAAGCCAT
  GTCTGACCGAATCTGCATTCACACCAATGGCCGAGTCAATGTGGAGGTGTCTGCTGAGGACCTGCTTACC
  TGCTGTGGTATCCAGTGTGGGGATGGCTGTAATGGTGGCTATCCCTCTGGAGCATGGAACTTCTGGACTA
  GAAAAGGCCTGGTTTCTGGTGGAGTATACAATTCTCATATAGGCTGCTTACCCTACACCATCCCTCCCTG
  TGAACACCATGTCAATGGCTCCCGTCCCCCATGCACTGGAGAAGGAGATACTCCCAAGTGCAACAAGATG
  TGTGAGGCTGGCTACTCCACATCCTACAAGGAAGATAAGCACTATGGGTACACTTCCTACAGTGTGTCTG
  ACAGCGAGAAGGAGATCATGGCGGAAATCTACAAAAATGGCCCAGTGGAGGGTGCTTTTACTGTGTTTTC
  TGACTTCTTGACTTACAAATCAGGCGTATACAAGCATGAAGCCGGTGATGTGATGGGAGGCCATGCCATC
  CGCATTCTGGGCTGGGGAATAGAGAATGGAGTACCCTACTGGCTGGTAGCAAACTCCTGGAACGTTGACT
  GGGGTGATAATGGTTTCTTTAAAATCCTCAGAGGAGAGAACCACTGTGGAATTGAATCAGAAATCGTGGC
  TGGAATCCCACGCACTCAGCAGTACTGGGGAAGATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212662 representing NM_022597
  Red=Cloning site Green=Tags(s)
  
  MWWSLIPLSCLLALTSAHDKPSFHPLSDDMINYINKQNTTWQAGRNFYNVDISYLKKLCGTVLGGPKLPE
  RVGFSEDINLPESFDAREQWSNCPTIAQIRDQGSCGSCWAFGAVEAMSDRICIHTNGRVNVEVSAEDLLT
  CCGIQCGDGCNGGYPSGAWNFWTRKGLVSGGVYNSHIGCLPYTIPPCEHHVNGSRPPCTGEGDTPKCNKM
  CEAGYSTSYKEDKHYGYTSYSVSDSEKEIMAEIYKNGPVEGAFTVFSDFLTYKSGVYKHEAGDVMGGHAI
  RILGWGIENGVPYWLVANSWNVDWGDNGFFKILRGENHCGIESEIVAGIPRTQQYWGRF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022597
• ORF Size: 1017 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022597.1, NM_022597.2, NP_072119.2
• RefSeq Size: 1967
• RefSeq ORF: 1020
• Locus ID: 64529
• MW: 37.5 kDa
• Gene Summary: lysosomal peptidase that is synthesized as a longer precursor and processed to the mature form [RGD, Feb 2006]