Brca1 (NM_012514) Rat Tagged ORF Clone

CAT#: RR212756

Brca1 (Myc-DDK-tagged ORF) - Rat breast cancer 1 (Brca1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Brca1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212756 representing NM_012514
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTATCTGCTGTTCGAATTCAAGAAGTACAAAATGTCCTTCATGCTATGCAGAAAATCTTGGAGT
  GTCCAATCTGTTTGGAACTGATCAAAGAACCGGTTTCCACACAGTGCGACCACATATTTTGCAAATTTTG
  TATGCTGAAACTCCTTAACCAGAAGAAAGGACCTTCCCAGTGTCCTTTGTGTAAGAATGAGATAACCAAA
  AGGAGCCTACAAGGAAGTGCAAGGTTTAGTCAACTTGTTGAAGAGCTGCTGAAAATCATTGATGCTTTTG
  AGCTTGACACCGGAATGCAGTGTGCAAATGGTTTTAGTTTTTCAAAAAAGAAAAATAGTTCTTCTGAGCT
  TCTGAATGAGGACGCTTCTATCATCCAGAGTGTGGGCTACCGAAACCGCGTCAAAAAACTTCAACAGATT
  GAGTCTGGAAGTGCCACCTTGAAGGACAGTCTCAGTGTCCAGTTGTCTAACCTTGGGATTGTGAGATCAA
  TGAAGAAAAATAGGCAGACACAACCTCAAAACAAATCTGTCTACATTGCTTTAGAATCTGATTCTTCTGA
  GGAGAGAGTAAATGCACCCGATGGCTGCAGTGTGAGAGATCAGGAATTGTTTCAGATCGCCCCTGGAGGA
  GCTGGAGATGAAGGCAAGTTGAACTCTGCAAAAAAGGCTGCTTGTGACTTTTCTGAGGGCATAAGAAACA
  TTGAACATCATCAATGCAGTGATAAAGATTTGAACCCCACTGAGAATCATGCAACTGAAAGGCATCCAGA
  AAAATGTCCGCGTATTTCTGTTGCAAACGTGCACGTGGAGCCGTGTGGCACAGATGCTCGTGCCAGCTCA
  TTACAGCGTGGGACCCGCAGTTTATTGTTCACTGAGGACAGACTGGATGCAGAAAAGGCTGAATTCTGTG
  ATAGAAGCAAACAGTCTGGCGCAGCAGTGAGCCAGCAGAGCAGATGGGCTGACAGTAAAGAAACATGTAA
  TGGCAGGCCGGTTCCCCGCACTGAGGGAAAGGCAGATCCAAATGTGGATTCCCTCTGTGGTAGAAAGCAG
  TGGAATCATCCGAAAAGCCTGTGCCCTGAGAATTCTGGAGCTACCACTGACGTTCCTTGGATAACACTGA
  ATAGCAGCATTCAGAAAGTGAATGAGTGGTTTTCCAGAACTGGTGAAATGTTAACTTCTGACAATGCATC
  TGACAGGAGGCCTGCGTCAAATGCAGAAGCTGCTGTTGTGTTAGAAGTTTCAAATGAAGTGGATGGATGT
  TTCAGTTCTTCAAAGAAAATAGACTTAGTTGCCCCTGATCCCGATAATGCTGTAATGTGTACAAGTGGAA
  GAGACTTCTCCAAGCCAGTAGAGAATATTATCAACGATAAAATATTTGGGAAAACCTATCAGAGAAAGGG
  AAGCCGCCCTCACTTGAACCATGTGACTGAAATTATAGGCACATTTACTACAGAACCACAGATTATACAA
  GAGCAGCCCTTCACAAATAAATTAAAACGCAAAAGAAGTACATGCCTTCATCCTGAGGACTTTATCAAGA
  AAGCAGATTTAACAGTTGTTCAAAGGATTTCTGAAAATTTAAATCAGGGAACTGACCAAATGGAGCCAAA
  TGACCAAGCAATGAGTATTACCAGTAACGGTCAGGAGAACAGAGCAACAGGTAATGATCTTCAGAGAGGG
  AGAAATGCTCATCCAATAGAATCATTGAGAAAGGAACCTGCTTTCACAGCTAAAGCCAAATCTATAAGCA
  ACAGTATAAGTGATTTGGAGGTAGAATTAAATGTTCACAGTTCAAAAGCACCTAAGAAAAATAGGCTGAG
  GAGGAAGTCTACCAGGTGTGTTCTTCCACTCGAACCAATCAGTAGAAATCCGAGCCCACCTACTTGTGCT
  GAACTTCAGATCGAGAGTTGTGGTAGCAGTGAAGAAACAAAGAAAAACAATTCCAACCAAACCCCAGCCG
  GGCACATTAGAGAGCCTCAACTCATCGAAGACACAGAACCCGCAGCTGATGCCAAGAAGAACGAGCCAAA
  TGAACACATAAGGAAGAGAAGTGCCAGTGATGCGTTCCCAGAAGAGAAATTAATGAACAAAGCTGGTTTA
  TTAACTAGCTGTTCAAGTCCTAGAAAGCCTCAAGGACCTGTCAATCCTAGCCCTGAGAGAAAAGGAATAG
  AGCAACTTGAAATGTGCCAGATGCCTGATAATAACAAAGAACTCGGGGATTTGGTCCTGGGAGGAGAGCC
  CAGTGGGAAACCTACTGAACCATCTGAGGAGAGCACCAGTGTGTCCTTGGTACCCGACACAGACTACGAC
  ACCCAGAACAGTGTCTCAATACTGGAAGCGAACACTGTCAGATATGCAAGAACAGGATCAGTTCAGTGTA
  TGACTCAGTTTGTCGCAAGTGAAAACCCCAAGGAACTTGTCCATGGTTCTAACAATGCTGGAAGTGGCTC
  GGAGTGCTTCAAGCACCCATTGAGACATGAACTTAACCACAATCAAGAGACAATAGAAATGGAAGACAGT
  GAACTTGATACTCAGTATTTGCAGAATACATTTCAAGTTTCAAAGCGTCAGTCATTTGCTTTATTTTCAA
  AACTTAGAAGTCCCCAAAAGGACTGTACTCTGGTAGGTGCCCGCTCTGTGCCCTCAAGGGAACCAAGTCC
  AAAGGTGACTTCTAGAGGTGAACAAAAAGAACGTCAGGGACAAGAAGAGTCTGAAATCAGTCATGTACAG
  GCAGTCACAGTCACAGTAGGCTTACCTGTGCCCTGTCAGGAAGGTAAGCCAGGTGCTGTTACAATGTGTG
  CTGATGTTTCTAGGCTTTGTCCGTCATCTCATTATAGAAGCTGTGAGAATGGACTCAACACCACAGATAA
  ATCTGGAATTTCACAAAACTCACATTTTAGACAATCAGTTTCTCCCCTCAGGTCATCTATAAAAACTGAC
  AATAGAAAAACTCTGACAGAGGGNCGATTTGAGAAACATACTGAAAGGGGRATGGGAAATGAGACTGCTG
  TTCAAAGTACAATACACACAATTAGTCTAAATAACAGAGGAGATGCTTGTCTAGAAGCCAGCTCAGGCAG
  TGTTATTGAAGTACATTCCACTGGTGAAAACGTCCAGGGGCAACTAGATAGAAACAGAGGGCCTAAGGTA
  AACACCGTGTCTCTATTAGATAGTACACAGCCTGGTGTCTCTAAGCAGAGTGCTCCTGTAAGTGATAAGT
  ATCTTGAAATAAAGCAGGAGAGTAAGGCTGTCAGTGCAGACTTCTCTCCATGTCTGTTCTCAGATCATCT
  TGAAAAACCTATGAGAAGTGATAAGACTTTTCAGGTTTGCTCTGAGACACCTGATGACCTGTTGGATGAT
  GTTGAAATACAGGAAAATGCTAGCTTCGGTGAAGGTGGCATAACGGAAAAGTCTGCTATTTTTAATGGAA
  GTGTCCTGAGAAGAGAGTCCAGTAGGAGCCCTAGCCCTGTAACCCATGCATCGAAGTCGCGGAGTCTCCA
  CAGAGGGTCTAGGAAATTAGAATTCTCAGAAGAGAGCGACTCCACTGAGGATGAAGACCTTCCCTGCTTC
  CAACACTTGCTGAGCAGAGTAAGCAGCACACCCGAACTTACCAGATGCAGCAGTGTGGTGACACAGCGTG
  TGCCAGAGAAAGCGAAGGGGACCCAAGCACCCCGGAAGAGTAGCATCAGTGACTGCAATAATGAGGTGAT
  CTTGGGAGAGGCATCTCAGGAATATCAGTTTAGTGAGGATGCAAAATGCTCTGGCAGCATGTTCTCCTCA
  CAGCACAGTGCTGCCCTAGGTTCNCCTGCAAATGCACTCTCCCAGGATCCCGATTTTAATCCACCTTCCA
  AACAAAGGCGTCACCAGGCTGAGAATGAGGAAGCCTTTCTAAGTGATAAGGAATTGATTTCAGATCATGA
  GGACATGGCAGCTTGCCTGGAAGAGGCTAGCGATCAAGAAGAGGACAGTATAATCCCGGACTCAGTGGCG
  TCTGGATATGAGAGTGAAGCAAACCTCTCTGAAGACTGCTCACAGAGTGATATTCTAACCACTCAGCAGA
  GGGCGACCATGAAGGATAACCTGATAAAGCTCCAGCAGGAAATGGCTCAGCTGGAAGCTGTGCTAGAGCA
  GCATGGGAGCCAGCCGTCTGGCCACCCCCCTTGCCTCCCAGCTGACCCTTGTGCCCTAGAAGACCTGCCA
  GATCCAGAACAAAACAGATCAGGAACAGCAATTTTAACTTCAAAGAACATTAATGAGAATCCTGTAAGCC
  AGAATCCAAAGCGCGCCTGTGATGACAAATCCCAACCACAACCTCCGGACGGTCTCCCCAGTGGAGATAA
  GGAGTCAGGAATGAGAAGGCCTTCCCCTTTTAAATCTCCACTGACCAGCAGTAGGTGCTCTGCACGAGGC
  CACTCCAGGAGTCTTCAGAATAGAAACTCCACSTCGCAAGAGGAGCTCCTTCAGCCTGCTGRATTGGAGA
  AGTCATGTGAACCACACAACTTAACAGGGCGGTCTTGCTTGCCAAGGCAAGATCTCGAAGGAACCCCATA
  CCCGGAATCTGGAATCAGGCTAGTCTCCAGCAGAGACCCTGATTCTGAATCCCCTAAAGTGTCAGCCCTT
  GTTTGCACTGCACCAGCTTCAACCTCTGCACTGAAAATATCCCAAGGTCAAGTTGCTGGGTCTTGCAGGA
  GTCCAGCTGCTGGTGGTGCTGATACTGCAGTGGTAGAAATTGTGAGCAAGATAAAGCCGGAAGTGACGTC
  TCCAAAAGAACGAGCGGAGAGAGATATATCCATGGTGGTGTCAGGCTTGACCCCTAAAGAAGTAATGATT
  GTCCAAAAGTTTGCTGAAAAATACCGCCTTGCTTTAACTGATGTAATTACTGAGGAAACTACGCATGTAA
  TTATAAAAACAGATGCTGAGTTTGTGTGTGAACGGACACTGAAATATTTTCTGGGCATTGCAGGAGGAAA
  GTGGATAGTTAGCTATTCTTGGGTGATCAAGTCTATCCAAGAAAGAAAACTTCTGAGTGTGCATGAATTT
  GAAGTCAAAGGAGACGTTGTGACTGGAAGCAACCACCAAGGTCCAAGGCGATCCAGAGAATCCCAGGAAA
  AGCTCTTTGAAGGCCTACAGATCTATTGTTGTGAGCCCTTCACCAACATGCCCAAAGATGAGCTGGAGAG
  GATGCTGCAGCTGTGTGGGGCTTCTGTGGTGAAGGAGCTTCCATTGCTCACCCGTGACACAGGTGCTCAT
  CCAATTGTGCTCGTGCAGCCAAGTGCCTGGACAGAAGACAACGACTGCCCTGATATTGGGCAGCTGTGCA
  AGGGACGTCTAGTGATGTGGGACTGGGTGTTGGACAGTATATCCGTCTACCGGTGTCGGGATCTGGATGC
  CTACCTGGTACAGAATATCACCTGTGGCCGTGATGGTAGCGAGCCACAAGACTCCAATGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212756 representing NM_012514
  Red=Cloning site Green=Tags(s)
  
  MDLSAVRIQEVQNVLHAMQKILECPICLELIKEPVSTQCDHIFCKFCMLKLLNQKKGPSQCPLCKNEITK
  RSLQGSARFSQLVEELLKIIDAFELDTGMQCANGFSFSKKKNSSSELLNEDASIIQSVGYRNRVKKLQQI
  ESGSATLKDSLSVQLSNLGIVRSMKKNRQTQPQNKSVYIALESDSSEERVNAPDGCSVRDQELFQIAPGG
  AGDEGKLNSAKKAACDFSEGIRNIEHHQCSDKDLNPTENHATERHPEKCPRISVANVHVEPCGTDARASS
  LQRGTRSLLFTEDRLDAEKAEFCDRSKQSGAAVSQQSRWADSKETCNGRPVPRTEGKADPNVDSLCGRKQ
  WNHPKSLCPENSGATTDVPWITLNSSIQKVNEWFSRTGEMLTSDNASDRRPASNAEAAVVLEVSNEVDGC
  FSSSKKIDLVAPDPDNAVMCTSGRDFSKPVENIINDKIFGKTYQRKGSRPHLNHVTEIIGTFTTEPQIIQ
  EQPFTNKLKRKRSTCLHPEDFIKKADLTVVQRISENLNQGTDQMEPNDQAMSITSNGQENRATGNDLQRG
  RNAHPIESLRKEPAFTAKAKSISNSISDLEVELNVHSSKAPKKNRLRRKSTRCVLPLEPISRNPSPPTCA
  ELQIESCGSSEETKKNNSNQTPAGHIREPQLIEDTEPAADAKKNEPNEHIRKRSASDAFPEEKLMNKAGL
  LTSCSSPRKPQGPVNPSPERKGIEQLEMCQMPDNNKELGDLVLGGEPSGKPTEPSEESTSVSLVPDTDYD
  TQNSVSILEANTVRYARTGSVQCMTQFVASENPKELVHGSNNAGSGSECFKHPLRHELNHNQETIEMEDS
  ELDTQYLQNTFQVSKRQSFALFSKLRSPQKDCTLVGARSVPSREPSPKVTSRGEQKERQGQEESEISHVQ
  AVTVTVGLPVPCQEGKPGAVTMCADVSRLCPSSHYRSCENGLNTTDKSGISQNSHFRQSVSPLRSSIKTD
  NRKTLTEXRFEKHTERXMGNETAVQSTIHTISLNNRGDACLEASSGSVIEVHSTGENVQGQLDRNRGPKV
  NTVSLLDSTQPGVSKQSAPVSDKYLEIKQESKAVSADFSPCLFSDHLEKPMRSDKTFQVCSETPDDLLDD
  VEIQENASFGEGGITEKSAIFNGSVLRRESSRSPSPVTHASKSRSLHRGSRKLEFSEESDSTEDEDLPCF
  QHLLSRVSSTPELTRCSSVVTQRVPEKAKGTQAPRKSSISDCNNEVILGEASQEYQFSEDAKCSGSMFSS
  QHSAALGXPANALSQDPDFNPPSKQRRHQAENEEAFLSDKELISDHEDMAACLEEASDQEEDSIIPDSVA
  SGYESEANLSEDCSQSDILTTQQRATMKDNLIKLQQEMAQLEAVLEQHGSQPSGHPPCLPADPCALEDLP
  DPEQNRSGTAILTSKNINENPVSQNPKRACDDKSQPQPPDGLPSGDKESGMRRPSPFKSPLTSSRCSARG
  HSRSLQNRNSXSQEELLQPAXLEKSCEPHNLTGRSCLPRQDLEGTPYPESGIRLVSSRDPDSESPKVSAL
  VCTAPASTSALKISQGQVAGSCRSPAAGGADTAVVEIVSKIKPEVTSPKERAERDISMVVSGLTPKEVMI
  VQKFAEKYRLALTDVITEETTHVIIKTDAEFVCERTLKYFLGIAGGKWIVSYSWVIKSIQERKLLSVHEF
  EVKGDVVTGSNHQGPRRSRESQEKLFEGLQIYCCEPFTNMPKDELERMLQLCGASVVKELPLLTRDTGAH
  PIVLVQPSAWTEDNDCPDIGQLCKGRLVMWDWVLDSISVYRCRDLDAYLVQNITCGRDGSEPQDSND
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012514
• ORF Size: 5451 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012514.1, NP_036646.1
• RefSeq Size: 5607
• RefSeq ORF: 5454
• Locus ID: 497672
• MW: 200.1 kDa
• Gene Summary: involved in the DNA damage response and DNA repair [RGD, Feb 2006]