Vav1 (NM_012759) Rat Tagged ORF Clone

CAT#: RR212778

Vav1 (Myc-DDK-tagged ORF) - Rat vav 1 guanine nucleotide exchange factor (Vav1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vav1
• Synonyms: Vav
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212778 representing NM_012759
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCTGGCGACAGTGCACCCACTGGCTGATCCAGTGTCGGGTGCTGCCTCCCAGCCACCGTGTGA
  CCTGGGAGGGGGCCCAGGTGTGTGAGCTGGCACAGGCACTGCGGGATGGTGTCCTCCTGTGTCAACTGCT
  TAACAACCTGCTCCCCCACGCCATCAATCTGCGGGAAGTTAACTTGTGGCCCCAGATGTCCCAGTTCCTT
  TGTCTTAAGAACATCCGAACCTTCCTGTCTACTTGCTGTGAGAAATTCGGCCTCAAGCGCAGCGAACTCT
  TTGAGGCTTTTGACCTCTTTGATGTGCAGGACTTTGGAAAGGTCATCTACACCCTGTCTGCCCTGTCATG
  GACACCCATTGCCCAGAACAAAGGGATCATGCCCTTCCCAACAGAGGACAGCGCCCTGGGTGATGAAGAT
  ATTTACAGTGGCCTTTCAGACCAGATTGATGACACCGCAGAGGAAGATGAGGACCTTTATGACTGCGTGG
  AGAATGAGGAGGCAGAGGGGGACGAGATCTACGAGGACCTAATGCGCTCGGAGTCGGTGCCCACACCTCC
  CAAGATGACAGAGTACGATAAGCGCTGCTGCTGCCTGCGGGAGATCCAGCAGACTGAGGAGAAGTACACA
  GACACACTGGGCTCCATCCAGCAGCACTTCATGAAGCCCCTCCAGCGATTCCTCAAGCCTCAAGACATGG
  AGACCATCTTTGTCAACATTGAGGAGCTGCTCTCTGTGCACACCCACTTCTTAAAGGAACTGAAGGATGC
  CCTGTCTGGCCCGGGAGCAACGATGCTGTATCAGGTCTTCATCAAGTATAAGGAGAGGTTCCTTGTTTAT
  GGCCGTTATTGCAGTCAGGTGGAGTCAGCCAGCAAGCACTTGGATCAAGTGGCCACAGCACGGGAGGATG
  TGCAGATGAAGCTGGAGGAATGCTCTCAAAGAGCCAACAATGGCCGCTTCACCCTGCGGGATCTGCTGAT
  GGTGCCTATGCAGCGGGTGCTGAAGTATCACCTCCTCCTCCAGGAGCTAGTGAAACACACACAGGATACT
  ACAGAAAAGGAGAACCTGCGGTTGGCCCTGGACGCCATGAGGGACCTGGCGCAGTGTGTGAATGAGGTCA
  AGAGGGACAATGAAACCTTACGGCAGATCACAAACTTTCAGCTGTCCATTGAGAACCTGGACCAGTCTCT
  GGCCAACTATGGCCGGCCTAAGATTGACGGTGAGCTCAAGATTACCTCAGTGGAACGGCGCTCCAAGACA
  GACAGGTATGCCTTCCTGCTGGACAAAGCACTGCTCATCTGTAAGCGCCGCGGGGACTCTTATGACCTCA
  AGGCCTCAGTGAACCTGCACAGCTTCCAAGTTCGGGATGACTCTTCCGGGGAGCGGGACAACAAGAAGTG
  GAGCCACATGTTCCTTCTGATTGAGGATCAAGGTGCCCAGGGGTATGAGCTGTTCTTCAAGACTCGGGAG
  CTAAAAAAGAAGTGGATGGAACAGTTTGAAATGGCCATCTCCAACATCTACCCAGAAAATGCTACAGCCA
  ATGGACATGATTTTCAGATGTTCTCCTTTGAGGAGACCACTTCCTGCAAGGCCTGCCAGATGTTACTCAG
  GGGCACATTCTACCAGGGATATCGCTGTTACAGGTGCCGTGCACCCGCACACAAGGAGTGTCTGGGAAGG
  GTGCCTCCATGTGGCCGTCAAGATTTCTCAGGAACCATGAAGAAGGACAAGCTACATCGAAGGGCCCAGG
  ACAAGAAAAGGAATGAATTGGGCCTTCCTAAGATGGAGGTGTGTCAGGAATACTACGGGATCCCTCCTCC
  TCCCGGAGCCTTTGGGCCATTTCTGCGGCTCAACCCTGGGGACATTGTGGAACTCACTAAGGCAGAGGCT
  GAACACAACTGGTGGGAGGGAAGAAATACAGCTACGAATGAAGTCGGCTGGTTTCCCTGTAACAGAGTTC
  GTCCCTACGTCCACGGCCCTCCCCAGGACCTGTCTGTGCACCTCTGGTATGCGGGCCCCATGGAGCGAGC
  AGGCGCCGAGGGCATCCTCACCAACCGCTCTGATGGGACCTACCTGGTGCGGCAAAGGGTGAAAGATACA
  GCGGAATTCGCCATCAGCATTAAGTATAATGTGGAGGTCAAGCATATTAAAATCATGACGTCAGAGGGGT
  TGTACCGCATCACAGAGAAGAAGGCTTTCCGGGGCCTCCCGGAACTGGTAGAGTTTTATCAGCAGAATTC
  CCTAAAAGATTGCTTCAAGTCGTTGGACACCACCTTGCAGTTTCCTTACAAGGAACCTGAGAGGAGAGCC
  ATCAACAAGCCGCCGGTTGGAAGCACCAAGTATTTTGGCACTGCCAAAGCCCGCTACGACTTCTGTGCCC
  GGGACCGATCGGAACTGTCCCTTAAGGAGGGTGACATCATCAAGATCCTCAACAAGAAGGGACAGCAAGG
  CTGGTGGCGTGGGGAGATCTATGGCCGGATTGGCTGGTTTCCCTCTAACTATGTGGAGGAAGACTATTCT
  GAATACTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212778 representing NM_012759
  Red=Cloning site Green=Tags(s)
  
  MELWRQCTHWLIQCRVLPPSHRVTWEGAQVCELAQALRDGVLLCQLLNNLLPHAINLREVNLWPQMSQFL
  CLKNIRTFLSTCCEKFGLKRSELFEAFDLFDVQDFGKVIYTLSALSWTPIAQNKGIMPFPTEDSALGDED
  IYSGLSDQIDDTAEEDEDLYDCVENEEAEGDEIYEDLMRSESVPTPPKMTEYDKRCCCLREIQQTEEKYT
  DTLGSIQQHFMKPLQRFLKPQDMETIFVNIEELLSVHTHFLKELKDALSGPGATMLYQVFIKYKERFLVY
  GRYCSQVESASKHLDQVATAREDVQMKLEECSQRANNGRFTLRDLLMVPMQRVLKYHLLLQELVKHTQDT
  TEKENLRLALDAMRDLAQCVNEVKRDNETLRQITNFQLSIENLDQSLANYGRPKIDGELKITSVERRSKT
  DRYAFLLDKALLICKRRGDSYDLKASVNLHSFQVRDDSSGERDNKKWSHMFLLIEDQGAQGYELFFKTRE
  LKKKWMEQFEMAISNIYPENATANGHDFQMFSFEETTSCKACQMLLRGTFYQGYRCYRCRAPAHKECLGR
  VPPCGRQDFSGTMKKDKLHRRAQDKKRNELGLPKMEVCQEYYGIPPPPGAFGPFLRLNPGDIVELTKAEA
  EHNWWEGRNTATNEVGWFPCNRVRPYVHGPPQDLSVHLWYAGPMERAGAEGILTNRSDGTYLVRQRVKDT
  AEFAISIKYNVEVKHIKIMTSEGLYRITEKKAFRGLPELVEFYQQNSLKDCFKSLDTTLQFPYKEPERRA
  INKPPVGSTKYFGTAKARYDFCARDRSELSLKEGDIIKILNKKGQQGWWRGEIYGRIGWFPSNYVEEDYS
  EYC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012759
• ORF Size: 2529 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012759.1, NM_012759.2, NP_036891.2
• RefSeq Size: 2917
• RefSeq ORF: 2532
• Locus ID: 25156
• MW: 98 kDa
• Gene Summary: guanine nucleotide exchange factor (GEF) from the Rho family of GTP binding proteins; plays a role in T-cell and B-cell development; can activate the c-Jun N-terminal kinase-1 (JNK1) pathway [RGD, Feb 2006]