Dlat (NM_031025) Rat Tagged ORF Clone

CAT#: RR212819

Dlat (Myc-DDK-tagged ORF) - Rat dihydrolipoamide S-acetyltransferase (Dlat), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dlat
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212819 representing NM_031025
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCGCGTCTGTGCGCGGAGGGTGCAGAGTGCTGTTCCCAGGGCCGGGTTCAGGGCTCGGTGGGCAA
  CTTTGAAGGGCCCGCGGACCGGCCCCGCCGCAGTTCGTTGCGGCAGTGGGATACCCAGCTATGGGGTCCG
  CTCGTTGTGCGGCTGGAGCTACGGCTCTGCCACCGTCCCACGTAACCGCATACTGCAACAACTCCTGGGG
  TCGCCCAGCCGCCGCTCTTATAGCCTCCCTCCGCACCAGAAGGTTCCACTACCTTCACTTTCCCCTACAA
  TGCAGGCAGGCACCATAGCCCGCTGGGAAAAAAAAGAAGGGGAAAAAATCAGTGAGGGTGATCTAATTGC
  AGAGGTTGAAACTGATAAAGCCACTGTTGGATTTGAGAGCTTGGAGGAGTGCTATATGGCAAAGATCCTT
  GTTCCTGAGGGGACCAGAGATGTTCCAGTAGGATCTATCATTTGTATCACAGTTGAAAAGCCTCAGGATA
  TTGAGGCCTTTAAAAATTATACATTGGATTCAGCAACAGCTGCCACACAGGCAGCCCCAGCCCCAGCTGC
  AGCTCCAGCTGCTGCCCCAGCTGCACCTTCTGCAAGTGCTCCAGGTAGCTCCTATCCCGTTCACATGCAG
  ATTGTTCTTCCTGCCCTCTCCCCAACCATGACCATGGGCACCGTCCAGAGGTGGGAAAAGAAAGTGGGAG
  AGAAGCTGAGTGAAGGAGACTTGCTGGCAGAGATAGAGACCGACAAGGCCACCATAGGCTTTGAAGTACA
  AGAAGAAGGTTATCTGGCAAAAATCCTGGTCCCTGAAGGCACAAGGGATGTTCCTCTGGGAACCCCGCTA
  TGTATCATAGTAGAAAAACAGGAAGATATAGCAGCATTTGCAGACTACAGGCCAACAGAAGTGACCAGCT
  TAAAGCCACAGGCACCACCACCTGTCCCACCCCCAGTGGCAGCTGTTCCTCCCATCCCCCAGCCTTTAGC
  ACCTACCCCCTCAGCCGCTCCTGCTGGACCAAAGGGAAGGGTGTTCGTTAGCCCTCTTGCAAAGAAATTG
  GCAGCAGAGAAAGGGATTGACCTCACCCAAGTTAAAGGGACGGGACCAGAAGGCAGAATCATCAAGAAGG
  ACATTGACTCTTTTGTGCCTACTAAGGCTGCTCCTGCCGCTGCAGCAGCTGCTCCCCCGGGTCCAAGAGT
  GGCACCAACTCCTGCAGGTGTCTTCATAGACATCCCCATCAGCAACATTCGTCGAGTGATTGCGCAGAGG
  CTCATGCAGTCGAAGCAGACTATACCTCATTATTACCTTTCTGTTGATGTAAATATGGGAGAGGTGCTGT
  TGGTGCGGAAGGAACTTAATAAGATGCTTGAAGGTAAAGGAAAAATCTCCGTCAATGACTTCATCATAAA
  AGCTTCAGCTTTGGCCTGCCTGAAAGTTCCTGAAGCAAACTCATCTTGGATGGACACAGTTATACGACAA
  AATCATGTGGTTGATGTCAGCGTTGCTGTCAGTACCCCTGCAGGACTTATCACCCCTATTGTGTTTAATG
  CACACATAAAAGGACTGGAAACCATTGCTAGTGATGTTGTTTCTTTAGCCTCCAAAGCAAGAGAGGGTAA
  ACTTCAGCCTCACGAGTTCCAGGGTGGGACATTTACAATCTCCAACTTAGGGATGTTCGGAATTAAGAAT
  TTCTCTGCGATTATTAACCCACCTCAGGCATGTATTTTGGCAATTGGTGCTTCCGAGGATAAACTGATCC
  CTGCAGATAATGAGAAAGGCTTTGACGTGGCTAGTGTGATGTCTGTCACACTCAGCTGTGATCATCGAGT
  TGTGGATGGAGCAGTTGGAGCCCAGTGGCTTGCTGAATTCAAAAAGTACCTTGAAAAGCCTGTAACTATG
  TTATTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212819 representing NM_031025
  Red=Cloning site Green=Tags(s)
  
  MWRVCARRVQSAVPRAGFRARWATLKGPRTGPAAVRCGSGIPSYGVRSLCGWSYGSATVPRNRILQQLLG
  SPSRRSYSLPPHQKVPLPSLSPTMQAGTIARWEKKEGEKISEGDLIAEVETDKATVGFESLEECYMAKIL
  VPEGTRDVPVGSIICITVEKPQDIEAFKNYTLDSATAATQAAPAPAAAPAAAPAAPSASAPGSSYPVHMQ
  IVLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGTPL
  CIIVEKQEDIAAFADYRPTEVTSLKPQAPPPVPPPVAAVPPIPQPLAPTPSAAPAGPKGRVFVSPLAKKL
  AAEKGIDLTQVKGTGPEGRIIKKDIDSFVPTKAAPAAAAAAPPGPRVAPTPAGVFIDIPISNIRRVIAQR
  LMQSKQTIPHYYLSVDVNMGEVLLVRKELNKMLEGKGKISVNDFIIKASALACLKVPEANSSWMDTVIRQ
  NHVVDVSVAVSTPAGLITPIVFNAHIKGLETIASDVVSLASKAREGKLQPHEFQGGTFTISNLGMFGIKN
  FSAIINPPQACILAIGASEDKLIPADNEKGFDVASVMSVTLSCDHRVVDGAVGAQWLAEFKKYLEKPVTM
  LL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031025
• ORF Size: 1896 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031025.1, NP_112287.1
• RefSeq Size: 2628
• RefSeq ORF: 1899
• Locus ID: 81654
• MW: 67.2 kDa
• Gene Summary: putative dihydrolipoamide acetyltransferase [RGD, Feb 2006]