Apoa1 (NM_012738) Rat Tagged ORF Clone

CAT#: RR212920

Apoa1 (Myc-DDK-tagged ORF) - Rat apolipoprotein A-I (Apoa1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Apoa1
• Synonyms: apoA-I
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212920 representing NM_012738
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGCTGCAGTGTTGGCTGTGGCCCTGGTCTTCCTGACAGGTTGCCAAGCTTGGGAGTTCTGGCAGC
  AAGATGAGCCCCAGTCCCAATGGGACAGGGTGAAGGATTTCGCCACTGTGTATGTGGATGCAGTCAAGGA
  CAGCGGCAGAGACTATGTGTCCCAGTTTGAATCCTCCACTTTGGGCAAACAGCTGAACCTGAATCTCCTG
  GACAACTGGGACACTCTGGGTTCAACTGTTGGTCGCCTACAGGAACAGCTAGGCCCAGTGACTCAGGAGT
  TCTGGGCTAACCTGGAGAAAGAAACAGATTGGCTGAGAAACGAGATGAACAAGGACCTGGAGAATGTGAA
  ACAGAAGATGCAGCCCCACCTGGATGAATTCCAGGAGAAGTGGAACGAGGAGGTCGAGGCCTACCGCCAG
  AAGCTGGAGCCTCTGGGCACCGAGCTTCACAAAAACGCGAAGGAGATGCAAAGGCATCTAAAGGTTGTGG
  CCGAGGAGTTTCGAGACCGCATGCGTGTGAATGCAGACGCGCTGCGCGCAAAGTTTGGGCTCTACAGCGA
  TCAGATGCGCGAGAACCTGGCCCAGCGCCTGACCGAGATCAAGAACCACCCTACCCTGATCGAGTATCAT
  ACCAAGGCCAGCGACCACCTGAAGACACTTGGTGAGAAAGCCAAACCCGCGCTGGATGACCTGGGCCAGG
  GCCTGATGCCGGTGCTGGAAGCCTGGAAGGCCAAAATCATGAGTATGATCGATGAGGCCAAAAAGAAGCT
  GAACGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212920 representing NM_012738
  Red=Cloning site Green=Tags(s)
  
  MKAAVLAVALVFLTGCQAWEFWQQDEPQSQWDRVKDFATVYVDAVKDSGRDYVSQFESSTLGKQLNLNLL
  DNWDTLGSTVGRLQEQLGPVTQEFWANLEKETDWLRNEMNKDLENVKQKMQPHLDEFQEKWNEEVEAYRQ
  KLEPLGTELHKNAKEMQRHLKVVAEEFRDRMRVNADALRAKFGLYSDQMRENLAQRLTEIKNHPTLIEYH
  TKASDHLKTLGEKAKPALDDLGQGLMPVLEAWKAKIMSMIDEAKKKLNA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012738
• ORF Size: 777 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012738.1, NP_036870.1
• RefSeq Size: 889
• RefSeq ORF: 780
• Locus ID: 25081
• MW: 30.1 kDa
• Gene Summary: major component of high density lipoprotein (HDL) that is involved in intercellular cholesterol transport in astrocytes; cofactor for lecithin cholesterolacyltransferase (LCAT) [RGD, Feb 2006]