Cbx6 (NM_001012119) Rat Tagged ORF Clone

CAT#: RR213008

Cbx6 (Myc-DDK-tagged ORF) - Rat chromobox homolog 6 (Cbx6), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cbx6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213008 representing NM_001012119
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGTCTGCAGTAGGCGAGTGGGTCTTCGCGGCCGAATCCATCATCAAACGCCGGATCCGAAAGG
  GACGCATCGAGTACCTGGTGAAATGGAAAGGGTGGGCGATCAAGTACAGCACTTGGGAGCCAGAGGAGAA
  CATTCTGGATTCGAGGCTCATCGCAGCCTTCGAGCAGAAGGAAAGGGAACGTGAGCTGTATGGGCCCAAG
  AAGAGGGGACCTAAACCCAAAACTTTCCTCCTAAAGCCAAGCGCCAGCGCCTCCTCGCCCAAGCTGCACT
  CCAGCGCTGCGGTGCACCGGCTCAAGAAAGACATCCGCCGCTGCCACCGTATGTCCCGCCGCCCCCTGCC
  ACGACCAGACCCACAAGGGGGTAACCCTGGCCTGCGCCCACCCATCTCGCCCTTTTCTGAGACCGTGCGC
  ATCATCAACCGCAAGGTGAAGCCCCGGGAACCCAAGCGGAACCGCATCATCCTGAACCTGAAGGTGATTG
  ACAAGGGCCCGGGAGGTGGCAGCACTGCCCAGGGCACCGGGGCACTGGCCCGCCCGAAAGTCCCCTCGCG
  GAACCGGGTCATTGGGAAGAGCAAGAAGTTCAGCGAGAGCATGCTCCGCACCCAGATCCGCCACATGAAG
  TTTGGCACCTTTGCACTCTACAAGCCCCCTCCCGCCCCTCTGGTGCCCTCTACTGCGGGCAAAGCTGACG
  TGGCCTCCTCGGGGCCTGGGCTGCTCCTGGCCACCCCAGCTGCTGCCCCCTTTGACGCACACAGCTCTAG
  CTCTTCCGGCTGCCCCTCACCCACACTGCAGTCCTCTGACCCAGATGATGCACCACCCAAGCTCCTCCCC
  GAGACCATAAGCCGATCTGCGTCCAACTGGCGAGAGTCAGAGGTGCTCGATCTGTCCATCCCTCCTGAGG
  CAGCTGCCACCGGCCAGCGGGTGCCTCCTGATGTCACTGCTGCTGCGGGCCAGGCGCTACACACAGTTCT
  GGAGGCCACAGGTACCGGCTCCTCCGAGCCTGAGGCTGGGGACTGGCGCCCCGAGATGTCGCCATGCTCC
  AATGTAGTCGTCACAGATGTCACCAGCAACCTCCTGACCGTGACCATCAAGGAATTCTGCAGCCCTGAGG
  ATTTCGAGAAGGTGGCTGCTGGGGTGGCAGGTGCTACAGGGGGTGGGGGTGGCACTGGGCCAAGTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213008 representing NM_001012119
  Red=Cloning site Green=Tags(s)
  
  MELSAVGEWVFAAESIIKRRIRKGRIEYLVKWKGWAIKYSTWEPEENILDSRLIAAFEQKERERELYGPK
  KRGPKPKTFLLKPSASASSPKLHSSAAVHRLKKDIRRCHRMSRRPLPRPDPQGGNPGLRPPISPFSETVR
  IINRKVKPREPKRNRIILNLKVIDKGPGGGSTAQGTGALARPKVPSRNRVIGKSKKFSESMLRTQIRHMK
  FGTFALYKPPPAPLVPSTAGKADVASSGPGLLLATPAAAPFDAHSSSSSGCPSPTLQSSDPDDAPPKLLP
  ETISRSASNWRESEVLDLSIPPEAAATGQRVPPDVTAAAGQALHTVLEATGTGSSEPEAGDWRPEMSPCS
  NVVVTDVTSNLLTVTIKEFCSPEDFEKVAAGVAGATGGGGGTGPSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012119
• ORF Size: 1188 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012119.1, NP_001012119.1
• RefSeq Size: 3095 bp
• RefSeq ORF: 1191 bp
• Locus ID: 315136
• Cytogenetics: 7q34
• MW: 42.5 kDa