Hspa14 (NM_001004257) Rat Tagged ORF Clone

CAT#: RR213029

Hspa14 (Myc-DDK-tagged ORF) - Rat heat shock protein 14 (Hspa14), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hspa14
• Synonyms: MGC94119
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213029 representing NM_001004257
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGATCGGCGTGCACCTGGGCTGCACATCAGCCTGTGTGGCTGTCTACAAGGATGGCCGGGCCG
  ATGTGGTTGCAAATGATGCGGGGGACAGAGTCACGCCAGCCGTCGTTGCTTACTCGGAACGTGAACAGGT
  GGTTGGACTGGCGGCAAAACAAAGTAGGATAAGGAATATCTCAAGCACGGTAGTGAAAGTAAAACAGATC
  CTTGGCAGAAGCACTGCTGATCCACAGGCTCAGAAATATATCTCGGAAAGTAAATGCTTAGTCATTGAGA
  AAAATGGGAAGTTGCAGTATGAAATAGATACAGGAGAAGAAACAAAACTCGTTAGTCCAGAAGATGTTGC
  CAGACTGATATTCAGTAAAATGAAAGAAACAGCACATTCTGTCCTGGGCTCAGATGCAAACGATGTCGTT
  GTTACCGTCCCATTTGACTTCGGAGAAAAGCAGAAGTCTGCTCTGGGAGAGGCAGCTGGAGCCGCCGGGT
  TTAATGTGTTGCGACTAATACATGAGCCGTCTGCAGCTCTCCTGGCCTATGGAATTGGACAAGACTGCCC
  TACCGGGAAGAGCAATGTTTTGGTGTTTAAGCTGGGAGGAACATCCTTGTCGCTCAGTATCATGGAAGTG
  AACAGCGGGATGTATCGGGTTCTTTCCACAAACACCAGTGACAACATCGGGGGTGTGCATTTCACAGACA
  GCTTAGCTCAGTACCTGGCTTCGGAGTTCCAGAGGCTGTTCAAGCACGATGTGAGAGGAAGTGCCCGGGC
  CATGATGAAGCTGATGAACAGCGCTGAGGTGGCCAAGCACTCCCTGTCCACCCTGGGAAGTGCCAATTGC
  TTCGTGGACTCCTTGTATGAAGGTCAAGACTTCGACTGCAATGTGTCCAGAGCAAGATTTGAGCTTCTTT
  GTTCTCCGCTTTTTAACAAATGTATAGAAGCAGTCCGGGCGCTCCTACAGCAGAGTGGATTTACAGCAGA
  TGACATTAACAAGGTTGTGCTGTGTGGTGGATCGTCAAGGATCCCAAGGCTGCAGCAGCTGATTAAAGAT
  CTCTTCCCAGCTGGGGACCTTCTCAACTCCATCCCTCCAGATGAGGTCATCCCTATCGGTGCAGCCATCG
  AGGCCGGGATTCTTGTTGGGAAGGAGAGCACCTCAGGGGATGACTCTGTCCTGATCGAGTGTTCAGCCCG
  GGATATTTTAGTTAAGGGAGTCGATGAATCAGGTGCCAACAGATTCACAGTGCTGTTCCCCTCTGGGACT
  CCTCTGCCAGCGCGAAGACAGCACACGCTACAAGCCCCTGGCAGTATATCTTCTGTTTGCCTTGAACTCT
  ATGAGTCTGAGGGCAAGAACTCTGTTAAAGAGGAGACCAAGTTTGCACAGGTTGTGCTCCAGGACTTAGA
  TAAAAAAGAAAATGGATTACGTGATATATTAGCTGTCCTTACTATGAAAAGGGATGGATCTTTACAGGTG
  ACGTGCACAGACCAAGAGACCGGGAAGTGTGAGGCCATCACTGTTGAAGTTGCATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213029 representing NM_001004257
  Red=Cloning site Green=Tags(s)
  
  MAAIGVHLGCTSACVAVYKDGRADVVANDAGDRVTPAVVAYSEREQVVGLAAKQSRIRNISSTVVKVKQI
  LGRSTADPQAQKYISESKCLVIEKNGKLQYEIDTGEETKLVSPEDVARLIFSKMKETAHSVLGSDANDVV
  VTVPFDFGEKQKSALGEAAGAAGFNVLRLIHEPSAALLAYGIGQDCPTGKSNVLVFKLGGTSLSLSIMEV
  NSGMYRVLSTNTSDNIGGVHFTDSLAQYLASEFQRLFKHDVRGSARAMMKLMNSAEVAKHSLSTLGSANC
  FVDSLYEGQDFDCNVSRARFELLCSPLFNKCIEAVRALLQQSGFTADDINKVVLCGGSSRIPRLQQLIKD
  LFPAGDLLNSIPPDEVIPIGAAIEAGILVGKESTSGDDSVLIECSARDILVKGVDESGANRFTVLFPSGT
  PLPARRQHTLQAPGSISSVCLELYESEGKNSVKEETKFAQVVLQDLDKKENGLRDILAVLTMKRDGSLQV
  TCTDQETGKCEAITVEVAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004257
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004257.1, NP_001004257.1
• RefSeq Size: 1761 bp
• RefSeq ORF: 1530 bp
• Locus ID: 307133
• Cytogenetics: 17q12.3
• MW: 54.5 kDa