Sphkap (NM_001127492) Rat Tagged ORF Clone

CAT#: RR213119

Sphkap (Myc-DDK-tagged ORF) - Rat SPHK1 interactor, AKAP domain containing (Sphkap), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sphkap
• Synonyms: RGD1311951; Skip
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213119 representing NM_001127492
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAACTCCCGGCTTTCTGTGCAAAGCCATGTGGGTAGTTGAACCAAACTCTGCTACCTGCCTTTCTG
  CTGCCCCGCCGGTCAAGAGACACTGCCAAGAATCCAGCAACGTGGAATCACCACTGATGCCCGAGGACTC
  GGAGCCACAGCAGATCACCAGCAGTGCAGCAGGCAGCCTGGCTGGCTCCATCACGGCCTGTAAGAAGGTT
  CTTCGAAGCAATAGCTTGCTGGAGTCCACAGACTACTGGTTGCAGAACCAGAGGACGCCTTGCCAGATTG
  GGTTTGTGGAAGACGAGTCTGAAAACTGTGCTTCTGTCTGCTTTGTGAACCTGGATGTCAACAAGGATGG
  CTGCAGCACAGAGAACCTGCAGCAGAAACTGGTCAACGTTTCACCAGACCTTCCAAAACTCATCAACTCC
  ATGAATGTCCAGAAGCCAAAAGAAAATGAAATTGTCCTCCTAAGTGGGCTAGCCTCTGGAAACCGCCAGG
  CAGATTTTGATGTTTCACAGTGCCCTTGGCTACCAGATATCTGCTTGGTCCAGTGCGCAAGAGGGAACAG
  ACCAAACAGTACCAACTGCATCATCTTTGAGATCAACAAGTTCTTAATTGGTCTGGAAGTGGTGCAGGAG
  CGACAGCTCCACCTGGAGACCAGCGTGTTGAAGCTGGAGGATGATACAAACTGTTCCCTGTCTTCGATTG
  AAGAAGATTTCCTTACAGCCTCAGAGCATTTGGAGGAAGAAATCGAGGTGGAAGACTGCAAAAGTGGTTT
  GGAAACTATAAATGTGTCAGCTAACGTTCTGGAAAGCAAAAGGCCAAAGGAAGCTACCCAGGAAGGATGG
  GATTACCACAAGGAAATATTGCATTGTGCTTTAGGTGAAAAACACATCAGAAAACACCGTATGCCCTCAA
  TGAAAACAGAAAGGTCAAAGGAAAACACAGCAGAGAACACAGCCCTGCAAAGTCTCAATCCTTCAGCCAG
  ACCATCACGCTTGAAAAGTGAAGTGGCGGGGAGCAAGCAGCCAGCCACGAACTACTCTTACCCCGAAAAT
  ATCAGAGGAGAAATAGAAACATCTCAGATGCTGTTCATCCCCAGGGATGCCTATCTCTCCATGGTTTCTT
  CATGTGGTGTACTCACTGAGCAGGGAAGCAACCACAGAGACCATGACGCCACTCCAAACTCCCTTCCTCC
  CGTTCAAAATGGAGAAGCCACCGCGGGCGAGTACGCTACCAACTTAGCAGAATCTGTAATGCAAGATGCC
  TTCATCCGATTGTCTCAGTCGCAACCTACACTCCCCCAGGAATCTGCAGTCAGTTTCTCTGTGGGAAGCG
  CTCTGCTCCCCAGTGGTTGTTGCACCAAAGATATGGTGGTCCCTCGTTCATGGAATGAGCTTCCTAAAAT
  TGTCATCGTGCAGAGCCCAGAGGGCAGCGACACAGCCCCTGAGCCAAGTGTCTCCTCCTGGCCCGACCTG
  GAAGTGTCTGTCGAGACCCCGGGCATCTTTTCTGAAGAGAGCTCCAGCAGACCCACTCAGAGTGCCCTAG
  AAGTTGCTTTGGCTTGTGCAGCCACTGTGATTGGAACCACTTCCAGTCCACAGGCCACAGAAAGATTCAC
  CATGGAGCAAGAATCTCTGGTATCTACCTACACCCAGAGAGGCAATGGGATACAGCAAACTCCAGTGCCC
  AGAGTATTCATGGGACCTTCTACAACTGAATACTCCTTCCCATCAGCTCTGTGTGGCATGACCCAAGTGG
  CAAGCGCTGTTGCTGTCTGTGGCCTAGGGGAAAGAGAAGAGGTGACATATTCTGTAGCTCCAAGTGACCT
  CCTGCCCACACCTGGGGCTTCTGAAGAAAGATCCTCCATTGGAAGTTTAGTGACAGAGGAAAGCACAGAA
  CTGGGGAAGGAAGCCATTGCAGAGGCTCTGCTCAGGGAGGCTACTCTGGTTTTAGCGAGGCCGAATGCAT
  ACAGCAGCGTCGGAGAGCTCTTGGAATCTGTGAACCGGAGGATCATAGAAACCACTTCTAAAACCCAGAT
  GCTGTGCACAGAAAATGTTCGAAGGAATGAACTGGCACACACCTTGTCCAATGTCATCCTCAAGCATTCC
  ATTGATGAGCTGCACCAGAAAAATATCATGGCTCACCCCACCGAGGAAAGGCATCCCGGTGGGACTCTGA
  ACACCTTAATGGAAAGTGTGAACCAGTTGCTGCACAACGTGATGTGCTTCACCTTCAGAAAGATGAATCA
  CATCGTAACACTGGGTGAGCATCCCTCATTTGATAAGGCTGCTGGTCATGCATGGGTTAAAGCCTCGGCT
  TGCTCCAGCAGCCATCCCTCTAGCAATGCACACGGTACTGGCCTTGTCATCAGAGATCTTGTAGAAGATG
  CATCTCCTAAGCCAGACAAGGGAGGAGCAAGGCCAGAACTGGTCAACAACCCCAGGCTGCAATCTGAATT
  CTCATGCAGTCACAGAATGCTTGATTCAACTGCTAGGACATTCCCCAAGGAAATGTATCCAAAAGGGATT
  GTGGGAGAGGATACAAGAAACCCTCTTCATACACTAAGTTATGACAGCAGTGAACAAAGAACTTCTACAG
  ATATAGGAAAATTAACAACAGTAGGAGAGGTCCGTAGTACCTTCCAGGAATCTGAAGACAGCATTGTTCC
  AAAAGCCCAAGAGAAACACACTTGTGCCACAACTCTAAACAACGAAGCTCAAATTAACCTATCCTTGTTA
  GGGGATGACCTAGTGGTTCCTGATCAATCTACACTAGAGGCAAAGCAGTCCGAGGTCTATGGCATCACAA
  ACTTTGCGGAAGAATTGGCAGAGACTGTTGTCTCCATGGCAACTGAAATCGCAGCAATCTGCCTTGACAA
  CTCCCATGGAAAACAGCCGTGGTTTTGTGCTTGGAAGAGAGGGAACGAATTTCTGATGACGCCTAACGCA
  TCCTGCCGATCCTTGAAGAGGAAGAAGGAAAACTCGGGCACGGGAAGCACTGTGAGGAAACACAAGCCAC
  CTCGCCTCAGTGAGATCAAGAGAAAAGCAGATGAGCACCCAGAGCTGAAAGAGAAGCTGATGAACAGAGT
  CATGGATGAGTCCATGAACCTCGAAGACATTCCCGATTCCGTCAGTACTTTTGCAAATGAAGTGGCAGCC
  AAGATCATGAACCTAACAGAATTCTCCACGGTGGATGGAGTGTGGCAAACCCAGAGTTGTTCCCGGAATC
  GGCTTCTGGGTGGTGATAGGTGGAACCGGCTGAAGGCCTCGAGCTGTGAAAGTATTCCGGAGGAGGACTC
  TGAAGCCAGGGTCTTTGTAAACAGCCTGGGTTTAATGAGTACCTTAAGCCAACCCGTTAGCAGGGCCAGC
  TCAGTCTCCAAACAGTCCAGCTGTGAGAGCATCACCGATGAGTTTTCCAGGTTCATGGTGAAACAGATGG
  AAAACGAAGGGAGGGGGTTCGAGTTGCTGCTGGATTACTACGCTGGCAAAAATGCCAGCAGTATCCTGAA
  CTCAGCAATCCAGCAGGCATGCCAGAAAAACGACCACCTCAACGTAAGACCAAGCTGCCCCTCTAAGCAG
  TCCAGCACAGAAAGCATAACAGAGGAGTTCTATAAGTACATGCTGAGAGACATTGCCAAAGAAAACAAAG
  ATGGCGCCCTGTCCAGACGAAGCAGCCATGATTGGAGCACGGGCTTGCTGTCTCCTTCCGCGCGATCCCC
  ACTGTGTTACAGGCAGTCATCTATGCCTGACAGCAGGTCCCCGTGCTCCAGGTTAACAGTAAATGCACCT
  ATCAAAGCCAACTCCTTAGATGGTTTTGCCCAAAACTGCCCTCAAGATTCTATAAATGTACAGCCAGTCA
  GTAGGGCTTCCTCGTCTGGCCTCTGCAAATCAGACTCCTGTTTATATCGCCGAAGTGGGACTGACCAGAT
  CACAAACATGCTAATTCATGAAACATGGGCCAGCTCCATTGAGGCTCTCATGAGAAAGAACAAAATCATT
  GCTGATGACGGCGAGGCAGCTAACGCTAGCCCTGGTCCTGTTTCTGGCGGCTCGCCTTCGCAAGTAGAGA
  AGTGTGCCAACAGACTAGTCACCGGCACAGGACACAAGGGACCAGCTCTACTTGTACAGGAGTCTGTTGA
  TTACCAGAGAAAGGATGCTGTCACTGAAGGCAATTGTTCCCCAGTGTCATCTCCAAGCAAAATGGCTCCT
  GTTAAAAAGCCCAGTGGTTTTGATCCTACAAGAGAAACCTCTGCATGCCACAATGCTGTTGCTCTCAAAA
  GCCCCAGGCGGTCTCTTTGCTCAAGGGAGGTGCCTTTGATTCAGATAGAGACCGATCAGAAAGAAGAATG
  CGTTGGAGAATCTGAAACCTTACTTCCCCAAAGTGGCTCCCTAGAGGAAGCAGAAGGGCCCCAGCCTGAG
  GAAACCATCCCAGATGTGGCCAGAAGCGAAGACACAGCCCTGAGCGCCTGTCAAAGCTCTCATGACAGCC
  TTGAGACCAGAGAAGAATTAGAAGTGGATGTCTTGAAAGAAGACATAACCCTAGATGAGTCCCGGAACCC
  TCCTAGCAGCAGCGAAGAGAGTACAGGCAGCTGGTCCCAGCTGGCCAATGAGGAGGACAACCCAGATGAC
  ACAAGTAGCTTTCTGCAGCTCAGTGAGCGGTCCATGAGCAATGGCAACACTAGTGGCACTAGCAGTCTTG
  GCATTATGGACCTGGACATTTATCAGGAAAGCATACCGTCTTCTCCCATGATTAATGAATTAGTAGAAGA
  AAAGGAGATTCTTAAAGAACAATCAGAAAGCGTAAAGGAACGTGCCTCTGGACTGCCAGGGAGAGCAGCC
  AGCCCCCAGAGGAGTCTACTGGTGATCAACTTTGACCTGGAGCCAGAGTGTCCTGATGCTGAGCTGCGAG
  CCACTCTGCAGTGGATAGCTGCCTCCGAGCTGGGCATCCCAACAATCTACTTTAAGAAATCTCAGGAAAG
  CAGAATTGAAAAGTTTCTAGATGTTGTGAAGCTTGTTCATCAGAAGTCCTGGAAGGTGGGAGATATATTT
  CATGCCGTTGTCCAGTACTGCAAAATGCATGCAGAGCAGAAAGAGGGAACTCCGAGTCTCTTTGACTGGC
  TCCTGGAACTAGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213119 representing NM_001127492
  Red=Cloning site Green=Tags(s)
  
  MSTPGFLCKAMWVVEPNSATCLSAAPPVKRHCQESSNVESPLMPEDSEPQQITSSAAGSLAGSITACKKV
  LRSNSLLESTDYWLQNQRTPCQIGFVEDESENCASVCFVNLDVNKDGCSTENLQQKLVNVSPDLPKLINS
  MNVQKPKENEIVLLSGLASGNRQADFDVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLEVVQE
  RQLHLETSVLKLEDDTNCSLSSIEEDFLTASEHLEEEIEVEDCKSGLETINVSANVLESKRPKEATQEGW
  DYHKEILHCALGEKHIRKHRMPSMKTERSKENTAENTALQSLNPSARPSRLKSEVAGSKQPATNYSYPEN
  IRGEIETSQMLFIPRDAYLSMVSSCGVLTEQGSNHRDHDATPNSLPPVQNGEATAGEYATNLAESVMQDA
  FIRLSQSQPTLPQESAVSFSVGSALLPSGCCTKDMVVPRSWNELPKIVIVQSPEGSDTAPEPSVSSWPDL
  EVSVETPGIFSEESSSRPTQSALEVALACAATVIGTTSSPQATERFTMEQESLVSTYTQRGNGIQQTPVP
  RVFMGPSTTEYSFPSALCGMTQVASAVAVCGLGEREEVTYSVAPSDLLPTPGASEERSSIGSLVTEESTE
  LGKEAIAEALLREATLVLARPNAYSSVGELLESVNRRIIETTSKTQMLCTENVRRNELAHTLSNVILKHS
  IDELHQKNIMAHPTEERHPGGTLNTLMESVNQLLHNVMCFTFRKMNHIVTLGEHPSFDKAAGHAWVKASA
  CSSSHPSSNAHGTGLVIRDLVEDASPKPDKGGARPELVNNPRLQSEFSCSHRMLDSTARTFPKEMYPKGI
  VGEDTRNPLHTLSYDSSEQRTSTDIGKLTTVGEVRSTFQESEDSIVPKAQEKHTCATTLNNEAQINLSLL
  GDDLVVPDQSTLEAKQSEVYGITNFAEELAETVVSMATEIAAICLDNSHGKQPWFCAWKRGNEFLMTPNA
  SCRSLKRKKENSGTGSTVRKHKPPRLSEIKRKADEHPELKEKLMNRVMDESMNLEDIPDSVSTFANEVAA
  KIMNLTEFSTVDGVWQTQSCSRNRLLGGDRWNRLKASSCESIPEEDSEARVFVNSLGLMSTLSQPVSRAS
  SVSKQSSCESITDEFSRFMVKQMENEGRGFELLLDYYAGKNASSILNSAIQQACQKNDHLNVRPSCPSKQ
  SSTESITEEFYKYMLRDIAKENKDGALSRRSSHDWSTGLLSPSARSPLCYRQSSMPDSRSPCSRLTVNAP
  IKANSLDGFAQNCPQDSINVQPVSRASSSGLCKSDSCLYRRSGTDQITNMLIHETWASSIEALMRKNKII
  ADDGEAANASPGPVSGGSPSQVEKCANRLVTGTGHKGPALLVQESVDYQRKDAVTEGNCSPVSSPSKMAP
  VKKPSGFDPTRETSACHNAVALKSPRRSLCSREVPLIQIETDQKEECVGESETLLPQSGSLEEAEGPQPE
  ETIPDVARSEDTALSACQSSHDSLETREELEVDVLKEDITLDESRNPPSSSEESTGSWSQLANEEDNPDD
  TSSFLQLSERSMSNGNTSGTSSLGIMDLDIYQESIPSSPMINELVEEKEILKEQSESVKERASGLPGRAA
  SPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQESRIEKFLDVVKLVHQKSWKVGDIF
  HAVVQYCKMHAEQKEGTPSLFDWLLELG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001127492
• ORF Size: 5124 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001127492.1, NP_001120964.1
• RefSeq Size: 5423
• RefSeq ORF: 5127
• Locus ID: 316561
• MW: 187.1 kDa