Akt2 (NM_017093) Rat Tagged ORF Clone

CAT#: RR213135

Akt2 (Myc-DDK-tagged ORF) - Rat v-akt murine thymoma viral oncogene homolog 2 (Akt2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Akt2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213135 representing NM_017093
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGAGGTATCTGTCATCAAAGAAGGCTGGCTCCACAAACGTGGTGAATACATCAAGACCTGGAGGC
  CCCGGTACTTCCTTCTGAAGAGTGATGGATCTTTTATTGGGTATAAAGAGAGACCTGAGGCCCCCGACCA
  GACCCTACCCCCCCTTAACAATTTCTCTGTAGCAGAATGCCAGCTGATGAAGACTGAGAGGCCACGACCC
  AACACCTTTGTCATACGCTGCCTGCAGTGGACCACAGTCATCGAGAGGACCTTCCATGTCGACTCTCCAG
  ATGAGAGGGAAGAGTGGATACGGGCTATCCAGATGGTAGCCAACAGTCTGAAGCAGCGGGGCCCCGGGGA
  GGATGCCATGGATTACAAGTGTGGCTCCCCCAGTGACTCTTCCACATCTGAGATGATGGAGGTAGCGGTC
  AGCAAGGCACGGGCCAAAGTGACCATGAACGACTTCGATTATCTCAAACTCCTCGGCAAGGGCACCTTCG
  GCAAAGTCATTCTAGTTCGAGAGAAGGCCACTGGCCGCTATTATGCCATGAAGATCCTGCGGAAGGAGGT
  CATCATTGCAAAGGATGAAGTCGCCCACACAGTCACAGAGAGCCGAGTCCTACAGAATACCAGGCACCCC
  TTCCTTACAGCCCTGAAGTATGCCTTCCAGACCCATGACCGCCTGTGCTTTGTGATGGAGTATGCCAACG
  GGGGCGACCTGTTTTTCCACCTCTCTCGGGAGCGAGTCTTCACGGAGGATCGGGCACGCTTTTATGGTGC
  AGAGATTGTGTCAGCTCTGGAGTACTTGCACTCGACGGATGTGGTGTACCGCGACATCAAGCTGGAAAAC
  CTTATGCTGGACAAAGATGGCCACATCAAGATCACTGACTTTGGCTTGTCCAAAGAGGGCATCAGTGATG
  GGGCCACCATGAAAACCTTCTGTGGTACCCCGGAGTACTTGGCGCCTGAGGTGCTAGAGGACAATGACTA
  CGGGCGTGCAGTGGACTGGTGGGGACTGGGCGTGGTCATGTACGAGATGATGTGCGGCCGCCTGCCCTTC
  TACAACCAGGACCACGAGCGCCTCTTCGAGCTCATCCTCATGGAGGAGATCCGGTTCCCACGCACGCTTG
  GGCCAGAGGCCAAGTCCCTGCTGGCTGGACTGCTCAAGAAGGACCCCAAGCAGAGGCTCGGTGGGGGTCC
  CAGTGATGCCAAGGAGGTCATGGAGCATCGGTTCTTCCTCAGCATCAACTGGCAGGATGTGGTACAGAAG
  AAGCTCCTGCCACCCTTCAAACCTCAGGTCACTTCAGAAGTGGACACAAGGTACTTTGATGATGAATTCA
  CCGCCCAGTCCATCACAATCACACCCCCAGACCGATATGACAGCCTGGGCTCGCTGGAACTGGACCAGCG
  CACACACTTCCCCCAGTTCTCCTACTCAGCCAGCATCCGAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213135 representing NM_017093
  Red=Cloning site Green=Tags(s)
  
  MNEVSVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLPPLNNFSVAECQLMKTERPRP
  NTFVIRCLQWTTVIERTFHVDSPDEREEWIRAIQMVANSLKQRGPGEDAMDYKCGSPSDSSTSEMMEVAV
  SKARAKVTMNDFDYLKLLGKGTFGKVILVREKATGRYYAMKILRKEVIIAKDEVAHTVTESRVLQNTRHP
  FLTALKYAFQTHDRLCFVMEYANGGDLFFHLSRERVFTEDRARFYGAEIVSALEYLHSTDVVYRDIKLEN
  LMLDKDGHIKITDFGLSKEGISDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPF
  YNQDHERLFELILMEEIRFPRTLGPEAKSLLAGLLKKDPKQRLGGGPSDAKEVMEHRFFLSINWQDVVQK
  KLLPPFKPQVTSEVDTRYFDDEFTAQSITITPPDRYDSLGSLELDQRTHFPQFSYSASIRE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017093
• ORF Size: 1443 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017093.1, NP_058789.1
• RefSeq Size: 1984
• RefSeq ORF: 1446
• Locus ID: 25233
• MW: 55.5 kDa
• Gene Summary: may be involved in phosphatidylinositol 3-kinase (PI3-K) mediated signaling [RGD, Feb 2006]