Stub1 (NM_001025625) Rat Tagged ORF Clone

CAT#: RR213174

Stub1 (Myc-DDK-tagged ORF) - Rat STIP1 homology and U-Box containing protein 1 (Stub1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Stub1
• Synonyms: Chip
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213174 representing NM_001025625
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGGCAAGGAGGAAAAGGAGGGCGGCGCGCGGCTGGGCACTGGTGGCGGCGGCAGCCCTGATAAGA
  GCCCGAGTGCGCAAGAGCTCAAGGAGCAGGGAAACCGGCTCTTCGTGGGCCGCAAGTACCCGGAGGCGGC
  GGCCTGCTACGGCCGCGCCATCACCCGGAACCCACTTGTGGCAGTGTACTACACCAACCGGGCCCTGTGC
  TATCTGAAGATGCAGCAGCCTGAACAGGCACTTGCTGACTGCCGGAGAGCCCTGGAGCTGGATGGGCAGT
  CTGTGAAGGCGCACTTCTTCCTGGGGCAGTGCCAGCTAGAGATGGAGAGTTATGATGAGGCCATTGCCAA
  TCTGCAGCGAGCCTATAGTTTGGCCAAGGAGCAGCGACTCAACTTTGGGGATGATATTCCTAGTGCCCTT
  CGCATTGCTAAGAAGAAGCGCTGGAACAGTATCGAGGAACGGCGCATCCACCAGGAGAGTGAGCTGCACT
  CCTATCTCACCAGGCTCATTGCTGCTGAGCGAGAGAGGGAACTGGAAGAGTGTCAGCGGAACCACGAGGG
  TGATGAGGATGATGGCCACATCAGGGCCCAGCAGGCCTGCATTGAGGCCAAGCACGATAAATACATGGCG
  GACATGAATGAGCTCTTCTCTCAGGTGGATGAGAAAAGAAAGAAGCGAGATATCCCTGACTACTTGTGTG
  GCAAGATCAGCTTTGAGCTGATGCGGGAACCCTGCATTACACCCAGTGGCATCACCTATGACCGCAAGGA
  CATTGAGGAGCACCTGCAGCGCGTGGGCCATTTCGATCCTGTGACCCGGAGCCCTCTGACCCAGGAACAG
  CTCATCCCCAACTTAGCCATGAAGGAAGTCATTGATGCTTTCATCTCTGAGAATGGCTGGGTAGAGGACT
  AC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213174 representing NM_001025625
  Red=Cloning site Green=Tags(s)
  
  MKGKEEKEGGARLGTGGGGSPDKSPSAQELKEQGNRLFVGRKYPEAAACYGRAITRNPLVAVYYTNRALC
  YLKMQQPEQALADCRRALELDGQSVKAHFFLGQCQLEMESYDEAIANLQRAYSLAKEQRLNFGDDIPSAL
  RIAKKKRWNSIEERRIHQESELHSYLTRLIAAERERELEECQRNHEGDEDDGHIRAQQACIEAKHDKYMA
  DMNELFSQVDEKRKKRDIPDYLCGKISFELMREPCITPSGITYDRKDIEEHLQRVGHFDPVTRSPLTQEQ
  LIPNLAMKEVIDAFISENGWVEDY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025625
• ORF Size: 912 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025625.2, NP_001020796.2
• RefSeq Size: 1283 bp
• RefSeq ORF: 915 bp
• Locus ID: 287155
• Cytogenetics: 10q12
• MW: 34.9 kDa