P2ry13 (NM_001002853) Rat Tagged ORF Clone

CAT#: RR213227

P2ry13 (Myc-DDK-tagged ORF) - Rat purinergic receptor P2Y, G-protein coupled, 13 (P2ry13), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: P2ry13
• Synonyms: P2y13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213227 representing NM_001002853
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCGGGACGGTCAACACCACTGGGATGCAGGGCTTCAACAAGTCTGAGAGGTGCCCCAGGGACACTC
  GGATGACACAGCTGCTGTTCCCAGTGCTCTATACTGTGGTCTTCTTCACAGGCGTCCTGCTGAACACCCT
  GGCCCTCTGGGTGTTCATCCACATCCCCAGCAATTCCACCTTTATCATCTACCTCAAAAACACCCTGGTG
  GCAGACTTGATAATGACACTAATGCTCCCTTTCAAAATCCTTTCTGACTCACGCCTTGCGCCCTGGCAGC
  TCAGGGGTTTTGTGTGCACTTTCTCATCCGTGGTCTTTTACGAGACAATGTATGTCGGCATCATGATGCT
  GGGTCTCATCGCTTTTGACAGATTCCTCAAGATCGTCGTACCGTTTAGGAAAACTTTTGTAAAAAAGACT
  GCTTTCGCAAAAATAGTCTCCATTTCCATCTGGCTCCTGATGTTCCTCATCTCCCTGCCGAACATGATCT
  TGAACAAAGAGGCAACGGCATCAACCGTGAAGAAATGTGCGTCCCTAAAGAGTCCCCTGGGGCTGCTGTG
  GCATCAAGTAGTCAGTCACACATGCCAGTTCATTTTCTGGACTGTGTTTATTCTGATGCTTCTGTTTTAC
  ACGGTGATTGCCAAGAAGGTATACGACTCCTATAGGAAGTTTAAAAGTAGGGACAGCAAGCACAAGCGGC
  TGGAGGCGAAGGTATTTATCGTCATGGCTGTCTTCTTTGTCTGCTTTGCCCCGTTTCATTTTGTCAGAGT
  ACCATACACCCACAGTCAAACCACCAACAAGACTGACTGTAGATTAGAAAACCAGCTGTTTCTTGCTAAA
  GAATCAACTCTCTTCCTGGCAACAACTAACATTTGTATGGACCCCTTAATATATATCATCTTGTGTAAGA
  AGTTCACCCGGAAGGTACCATGTATGAGATGGAGGACAAAGACAGCGGCGTCCAGCGATGAGCACCACAG
  CAGTCAGACAGACAACATCACCCTATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213227 representing NM_001002853
  Red=Cloning site Green=Tags(s)
  
  MLGTVNTTGMQGFNKSERCPRDTRMTQLLFPVLYTVVFFTGVLLNTLALWVFIHIPSNSTFIIYLKNTLV
  ADLIMTLMLPFKILSDSRLAPWQLRGFVCTFSSVVFYETMYVGIMMLGLIAFDRFLKIVVPFRKTFVKKT
  AFAKIVSISIWLLMFLISLPNMILNKEATASTVKKCASLKSPLGLLWHQVVSHTCQFIFWTVFILMLLFY
  TVIAKKVYDSYRKFKSRDSKHKRLEAKVFIVMAVFFVCFAPFHFVRVPYTHSQTTNKTDCRLENQLFLAK
  ESTLFLATTNICMDPLIYIILCKKFTRKVPCMRWRTKTAASSDEHHSSQTDNITLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001002853
• ORF Size: 1008 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002853.1, NP_001002853.1
• RefSeq Size: 1011 bp
• RefSeq ORF: 1011 bp
• Locus ID: 310444
• Cytogenetics: 2q26
• MW: 38.8 kDa
• Gene Summary: G-protein coupled receptor activated by ADP and 2MeSADP and inhibited by P2Y antagonist AR-C69931MX [RGD, Feb 2006]