Aldh3b1 (NM_001006998) Rat Tagged ORF Clone

CAT#: RR213434

Aldh3b1 (Myc-DDK-tagged ORF) - Rat aldehyde dehydrogenase 3 family, member B1 (Aldh3b1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aldh3b1
• Synonyms: MGC95025
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213434 representing NM_001006998
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCGTTTGAAGATAAGCTTCAGCAGCTGCGAGAGGCCTTCAATGCCGGGAGGACGAGGTCAGCTG
  AGTTCCGGGCTGCACAGCTGCAAGGCCTGAGCCACTTCCTCAGAGACAACAAACAGCAGCTGCAGGAGGC
  GCTGGCCCAAGACCTACACAAGTCAGCCTTTGAGTCGGAGGTGTCCGAGATTGCCATCAGTCAGGCTGAG
  GTGGACCTGGCCCTCAGGAACCTGCGGTCTTGGATGAAGGATGAGAAAGTGTCCAAGAATCTGGCCACAC
  AGCTGGACTCAGCCTTCATCCGGAAGGAGCCCTTCGGGCTGGTCCTCATCATCGTGCCCTGGAATTACCC
  CTTGAACCTGACACTCGTGCCTCTGGTGGGGGCCATTGCTGCAGGGAATTGTGTGGTACTCAAACCCTCA
  GAGATCAGCAAGGCCACTGAAAAGATCCTGGCTGAGGTCCTGCCCCGCTATCTGGACCAGAGCTGCTTCG
  CTGTGGTGCTGGGTGGGCCTCAGGAAACTGGGCAGCTGCTGGAACACAGGTTCGACTACATCTTCTTCAC
  AGGGAACACTTATGTAGGCAAGATCGTCATGGCTGCTGCTGCCAAACACCTGACGCCCATCACCCTGGAG
  CTGGGGGGTAAGAACCCCTGCTATGTGGATGACAACTGCGACCCCCAGACAGTGGCCAACCGTGTGGCCT
  GGTTCCGCTACTTTAACGCCGGCCAGACCTGTGTGGCCCCGGATTATGTCCTGTGTAGCCAGGAGATGCA
  GGAGCGGCTGGTGCCCGCCCTGCAGAATGCCATCACGCGTTTCTATGGAGACAACCCACAGACCTCCCCC
  AACCTGGGCAGAATCATCAACCAGAAGCATTTCGAGCGGCTCCAGGGATTGCTGGGCTGTGGCCGTGTGG
  CCATCGGGGGCCAGAGCGATGAGGGGGAGCGCTACATTGCCCCCACAGTGTTAGTGGATGTGCAAGAGAC
  AGAGCCCGTGATGCAGGAGGAGATCTTTGGACCCATCCTGCCCCTGGTGACTGTGACGAACCTGGATGAG
  GCAATTGAGTTCATCAACCGGAGGGAGAAGCCACTTGCGCTGTACGCCTTCTCTAAGAGAAGCCAGGTCA
  TCAAACAGGTGTTGGCCCGGACCAGCAGTGGGGGCTTCTGCGGGAACGATGGCTTCATGCACATGACCCT
  GTCCAGCCTTCCTTTTGGAGGAGTGGGAACCAGCGGGATGGGCAGGTACCACGGCAAGTTCTCCTTTGAC
  ACCTTTTCCAACCAGCGTGCCTGTTTGCTGCGCAGCCCTGGGATGGAGAAAATCAACGACCTGCGCTACC
  CGCCCTACACCAGTCGCAACCTCAGGGTGTTGCTGGTGGCCATGGAAAAGAGATGCTGCAGCTGCACGCT
  GCTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213434 representing NM_001006998
  Red=Cloning site Green=Tags(s)
  
  MDSFEDKLQQLREAFNAGRTRSAEFRAAQLQGLSHFLRDNKQQLQEALAQDLHKSAFESEVSEIAISQAE
  VDLALRNLRSWMKDEKVSKNLATQLDSAFIRKEPFGLVLIIVPWNYPLNLTLVPLVGAIAAGNCVVLKPS
  EISKATEKILAEVLPRYLDQSCFAVVLGGPQETGQLLEHRFDYIFFTGNTYVGKIVMAAAAKHLTPITLE
  LGGKNPCYVDDNCDPQTVANRVAWFRYFNAGQTCVAPDYVLCSQEMQERLVPALQNAITRFYGDNPQTSP
  NLGRIINQKHFERLQGLLGCGRVAIGGQSDEGERYIAPTVLVDVQETEPVMQEEIFGPILPLVTVTNLDE
  AIEFINRREKPLALYAFSKRSQVIKQVLARTSSGGFCGNDGFMHMTLSSLPFGGVGTSGMGRYHGKFSFD
  TFSNQRACLLRSPGMEKINDLRYPPYTSRNLRVLLVAMEKRCCSCTLL
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001006998
• ORF Size: 1404 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001006998.1, NP_001006999.1
• RefSeq Size: 1913 bp
• RefSeq ORF: 1407 bp
• Locus ID: 309147
• Cytogenetics: 1q43
• MW: 52.1 kDa