Pkn2 (NM_001105755) Rat Tagged ORF Clone

CAT#: RR213438

Pkn2 (Myc-DDK-tagged ORF) - Rat protein kinase N2 (Pkn2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pkn2
• Synonyms: Pak-2; Prkcl2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213438 representing NM_001105755
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCAACCCCGACCGGGGGGAGATCCTCCTCACGGAGCTGCAGGGAGATTCCCGACCTCTTCCAT
  TTTCTGAGAATGTGAGTGCTGTTCAGAAATTAGACTTTTCAGATACAATAGTGCAACAGAAATTGGATGA
  CGTCAAGGATCGAATAAAGAGAGAAATAAGGAAAGAACTGAAAATCAAAGAAGGAGCTGAAAACCTGAGG
  AAAGTTACAACAGATAAGAAAAATTTGGCTTATGTAGACAACATTTTGAAAAAGTCAAATAAAAAATTAG
  AAGAATTACATCACAAGCTACAAGAATTAAATGCACACATTGTTGTATCGGATCCAGAGGACTCTGCAGA
  TTGTCCGAGGACTCCAGATACTCCAAATAGTGACTCTCGTAGTTCTACTAGCAACAATAGATTGATGGCC
  TTACAGAAACAACTGGATATAGAACTTAAAGTAAAGCAAGGTGCAGAGAACATGATACAGATGTACTCAA
  ATGGCCCTTCAAAGGATCGGAAACTCCATGGTACAGCTCAGCAACTGCTCCAGGATAGCAAGACAAAAAT
  AGAAGTCATCCGAATGCAGATTCTTCAGGCCGTCCAGACCAATGAGCTGGCTTTTGATAATGCAAAACCT
  GTGATAAGCCCTCTTGAGCTTCGAATGGAAGAATTAAGACATCATTTTAGGATAGAATTTGCAGTAGCCG
  AAGGTGCAAAGAATGTCATGAAATTACTTGGCTCAGGAAAAGTAACAGACAGAAAAGCGCTTTCAGAAGC
  CCAAGCAAGATTTAATGAATCAAGTCAGAAGTTGGACCTTTTAAAATATTCACTAGAGCAGAGGTTAAAT
  GAACTCCCCAAGAATCACCCCAAAAGCAGTGTCGTCATTGAGGAGCTCTCCCTTGTTGCATCGCCAACCC
  TGAGTCCACGACAGAGTATGCTGTCCACGCAGAACCAGTACAGCACACTGTCCAAGCCAGCAGCACTAAC
  CGGTACTTTGGAAGTTCGTCTCATGGGGTGCCAAGATATCCTGGAGAATGTCCCTGGACGGTCAAAAGCG
  ACATCCGTAGCACTGCCTGGATGGAGTCCAAGTGAAAACAGATCATCGTTTATGAGCAGAACTAGTAAAA
  GTAAAAGTGGAAGCAGTCGCAACCTCCTGAAAACCGACGACTTGTCCAATGATGTTTGTGCTGTTTTGAA
  GCTAGATAATACTGTGGTTGGTCAAACTATCTGGAAGCCCATTTCCAATCAGTCGTGGGACCAGAAGTTT
  ACACTGGAACTAGACAGGTCACGTGAACTGGAAATTTCAGTTTATTGGCGTGATTGGAGATCTCTGTGTG
  CTGTAAAATTTCTGAGGTTAGAAGATTTTTTAGACAACCAGCGGCATGGCATGTGTCTCTATTTGGAACC
  ACAGGGTACTTTGTTTGCAGAGGTTACTTTTTTTAATCCAGTTATTGAAAGACGACCAAAACTCCAAAGA
  CAAAAGAAAATTTTTTCAAAACAACAAGGCAAAACATTTCTCAGAGCCCCTCAAATGAATATTAATATTG
  CCACTTGGGGAAGGCTAGTGAGACGAGCTATTCCCACAGTAAATCATTCTGGCACGTTCAGCCCTCAGAC
  TCCTGTGCCCGCTACAGTGCCAGTGGTGGATGCACGCACTCCTGAACTCGCACCTCCAGCCAGTGATTCT
  ACAGTAACCAAATTGGACTTTGATCTTGAACCTGAAGCTCCTCCAGCCCCACCACGTGCTTCTTCCCTTG
  GAGAGATAGATGACTCTTCTGAATTGAGAGTTTTGGATATACCTGGACAGGGTTCAGAAACTGTTTTCGA
  TATTGAGAATGACAGAAATAACATGCGTCCAAAATCCAAATCTGAATATGAGCTTAACATTCCTGACTCA
  AGTCGAAGCTGTTGGAGTGTTGGAGAACTTGAGGACAAGAGATCTCAACAGAGGTTTCAGTTTAATCTAC
  AAGACTTCAGGTGCTGTGCTGTCTTGGGGAGAGGACACTTTGGAAAGGTGCTGTTGGCAGAATATAAACA
  CACAAATGAAATGTTTGCAATAAAAGCCTTAAAGAAAGGGGACATTGTGGCACGAGATGAAGTAGACAGC
  TTGATGTGTGAAAAAAGAATTTTTGAGACTGTGAACAGTGTCCGGCATCCTTTCCTAGTGAACCTGTTTG
  CATGTTTCCAAACCAAAGAGCATGTTTGCTTTGTGATGGAGTACGCTGCTGGTGGGGACCTGATGATGCA
  CATCCACACTGACGTCTTCTCTGAACCCAGAGCTGTATTTTATGCAGCATGTGTAGTTCTTGGATTGCAA
  TATTTACATGAACACAAAATTGTTTATAGAGACTTGAAGTTGGATAACCTGTTGCTGGATACAGAAGGCT
  TCGTGAAGATTGCTGACTTTGGTCTTTGCAAAGAAGGAATGGGGTATGGAGATAGGACAAGCACATTTTG
  TGGTACTCCTGAATTTCTTGCCCCAGAAGTATTAACAGAAACGTCGTACACAAGGGCTGTAGATTGGTGG
  GGCCTTGGCGTACTTATATATGAAATGCTCGTCGGAGAGTCTCCCTTTCCTGGTGATGATGAAGAAGAGG
  TTTTTGACAGTATTGTAAATGACGAAGTAAGGTATCCAAGGTTCTTATCTACAGAAGCCATTTCCATAAT
  GAGAAGGCTGTTGAGGAGGAATCCTGAGCGGCGCCTGGGAGCTGGTGAGAAAGATGCCGAGGATGTAAAG
  AAGCACCCGTTTTTCCGGCTAACCGACTGGAGTGCACTACTGGACAAGAAAGTAAAGCCACCATTTGTAC
  CTACCATTAGAGGACGGGAAGATGTTAGTAACTTTGATGATGAATTTACCTCAGAAGCACCTATTCTGAC
  TCCCCCTCGAGAACCGAGGATACTCTTGGAGGAGGAGCAGGAGATGTTCCGCGATTTTGACTACGTTGCT
  GATTGGTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213438 representing NM_001105755
  Red=Cloning site Green=Tags(s)
  
  MASNPDRGEILLTELQGDSRPLPFSENVSAVQKLDFSDTIVQQKLDDVKDRIKREIRKELKIKEGAENLR
  KVTTDKKNLAYVDNILKKSNKKLEELHHKLQELNAHIVVSDPEDSADCPRTPDTPNSDSRSSTSNNRLMA
  LQKQLDIELKVKQGAENMIQMYSNGPSKDRKLHGTAQQLLQDSKTKIEVIRMQILQAVQTNELAFDNAKP
  VISPLELRMEELRHHFRIEFAVAEGAKNVMKLLGSGKVTDRKALSEAQARFNESSQKLDLLKYSLEQRLN
  ELPKNHPKSSVVIEELSLVASPTLSPRQSMLSTQNQYSTLSKPAALTGTLEVRLMGCQDILENVPGRSKA
  TSVALPGWSPSENRSSFMSRTSKSKSGSSRNLLKTDDLSNDVCAVLKLDNTVVGQTIWKPISNQSWDQKF
  TLELDRSRELEISVYWRDWRSLCAVKFLRLEDFLDNQRHGMCLYLEPQGTLFAEVTFFNPVIERRPKLQR
  QKKIFSKQQGKTFLRAPQMNINIATWGRLVRRAIPTVNHSGTFSPQTPVPATVPVVDARTPELAPPASDS
  TVTKLDFDLEPEAPPAPPRASSLGEIDDSSELRVLDIPGQGSETVFDIENDRNNMRPKSKSEYELNIPDS
  SRSCWSVGELEDKRSQQRFQFNLQDFRCCAVLGRGHFGKVLLAEYKHTNEMFAIKALKKGDIVARDEVDS
  LMCEKRIFETVNSVRHPFLVNLFACFQTKEHVCFVMEYAAGGDLMMHIHTDVFSEPRAVFYAACVVLGLQ
  YLHEHKIVYRDLKLDNLLLDTEGFVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTETSYTRAVDWW
  GLGVLIYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSTEAISIMRRLLRRNPERRLGAGEKDAEDVK
  KHPFFRLTDWSALLDKKVKPPFVPTIRGREDVSNFDDEFTSEAPILTPPREPRILLEEEQEMFRDFDYVA
  DWC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001105755
• ORF Size: 2949 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001105755.2, NP_001099225.2
• RefSeq Size: 5592
• RefSeq ORF: 2952
• Locus ID: 207122
• MW: 111.7 kDa
• Gene Summary: phospholipid-regulated protein kinase, phosphorylates ribosomal protein S6; may play a role in hepatic regulation [RGD, Feb 2006]