Amz2 (NM_001014121) Rat Tagged ORF Clone

CAT#: RR213493

Amz2 (Myc-DDK-tagged ORF) - Rat archaelysin family metallopeptidase 2 (Amz2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Amz2
• Synonyms: RGD1304846
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213493 representing NM_001014121
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGTACTACGGCACTCCGAGCACACACTGAAAACAGCTCTCCTCTCAAAGAACCCAGACCTCGTGT
  CCCAGTACGAGAAGTTAGACGCGGGGGAGCAACGGTTAATGAATGAAGCCTTCCAGCCACGCAATAATCT
  ATTCGAACCCATTACCTTGCATTCACAGTCAGACTGGATCTCCTCCCACCCCGAGGCTCCCCAAGACTTC
  GAGCAGTTTTTCTCTGACCGCTACAGGAAGGCACCCTGCCCGAAGAAACACATTATCTACATTCAGCCCA
  TTGGCTTTCTAGGCAACACCAGAGTTATCAGTGAGGAGTATATTAAATGGCTCAAGGGCTACTGCGAAGC
  CTTTTTCTACGGTTTGAAGGTGAAGTTCTTAGAACCGGTTTCTGTCTCTGCGACTAAGTGCTCCTTTAGA
  GTGAACGAGAACACACAGAACCTGCAGATCCACACAGGGCACATCCTGGCATTCTTGAAGAGGAATAAAC
  CCGAAGATGCCTTCTGCATCGTGGGGATAACGATGATCGACCTCTACCCTAGAGACTCGTGGAACTTCGT
  CTTCGGCCAGGCCTCCCTGTCAAGTGGCGTGGGCATATTCAGCTTTGCCAGGTACGGCAAGGACTTCTAC
  ACCTCGAAATATGAAGGCAGCGTGAAGGTTCCCCAGCGGACAGTCTCCAGTGACTACTCCATTTTTGATA
  ACTACTACATCCCTGAGATCACTAGTGTTTTGCTGCTGCGATCCTGTAAGACTTTAACCCACGAGATCGG
  ACACATACTTGGATTGCGACACTGCCAGTGGCTTGCGTGCTTAATGCAAGGCTCCAATCACTTGGAGGAA
  TCTGACCGTCGTCCTCTAAATGTCTGCCCTATCTGCTTACGTAAGCTGCAGAGTGCTATCGGCTTCAATA
  TTGTGGAGAGATACAAAGCCCTGGTGAAGTGGATTGACGATGAGTCTTGCGGCGAGTCAGGAGCGACTCC
  GAAAAGCAGCTCTGAGCATGTGTATTTGCCGAAGCCTGTGGAAGCCTTCAAGGACTGGAGAGAGTGGATA
  CTCAGATGCATTGCTGTTCTTGAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213493 representing NM_001014121
  Red=Cloning site Green=Tags(s)
  
  MQVLRHSEHTLKTALLSKNPDLVSQYEKLDAGEQRLMNEAFQPRNNLFEPITLHSQSDWISSHPEAPQDF
  EQFFSDRYRKAPCPKKHIIYIQPIGFLGNTRVISEEYIKWLKGYCEAFFYGLKVKFLEPVSVSATKCSFR
  VNENTQNLQIHTGHILAFLKRNKPEDAFCIVGITMIDLYPRDSWNFVFGQASLSSGVGIFSFARYGKDFY
  TSKYEGSVKVPQRTVSSDYSIFDNYYIPEITSVLLLRSCKTLTHEIGHILGLRHCQWLACLMQGSNHLEE
  SDRRPLNVCPICLRKLQSAIGFNIVERYKALVKWIDDESCGESGATPKSSSEHVYLPKPVEAFKDWREWI
  LRCIAVLEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014121
• ORF Size: 1077 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014121.1, NP_001014143.1
• RefSeq Size: 2963 bp
• RefSeq ORF: 1080 bp
• Locus ID: 360650
• Cytogenetics: 10q32.1
• MW: 41.4 kDa