Usp7 (NM_001024790) Rat Tagged ORF Clone

CAT#: RR213504

Usp7 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 7 (herpes virus-associated) (Usp7), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Usp7
• Synonyms: Hausp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213504 representing NM_001024790
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCACCAACAGCAGCAGCAGCAGCAGCAGAAAGCGGGCGAGCAGCAGCTGAGCGAGCCCGAGGACA
  TGGAGATGGAAGCTGGAGATACAGATGACCCACCAAGAATTACTCAGAACCCCGTCATCAATGGAAATGT
  AGCCCTGAGTGATGGGCACAGCAACGCAGAGGAGGACATGGAGGACGACACCAGTTGGCGCTCCGAGGCA
  ACCTTTCAGTTCACTGTCGAGCGCTTCAGCAGACTGAGTGAGTCGGTCCTTAGCCCTCCGTGTTTTGTGC
  GAAATCTGCCATGGAAGATTATGGTGATGCCACGCTTTTATCCAGACAGACCGCACCAAAAAAGCGTAGG
  ATTCTTTCTCCAGTGCAATGCTGAATCTGACTCCACGTCGTGGTCCTGCCATGCACAAGCAGTGCTGAAG
  ATAATAAATTACAGGGATGACGACAAGTCATTCAGCCGGCGAATCAGCCACTTGTTCTTCCACAAGGAAA
  ACGACTGGGGATTCTCTAATTTCATGGCCTGGAGTGAAGTGACTGATCCTGAGAAAGGATTTATAGATGA
  TGACAAAGTCACCTTTGAAGTTTTTGTACAGGCGGATGCTCCCCATGGAGTTGCGTGGGACTCAAAGAAG
  CATACTGGCTATGTCGGCTTAAAGAATCAGGGAGCGACGTGTTACATGAATAGCTTGCTGCAGACCTTGT
  TTTTCACAAATCAGTTACGAAAGGCTGTGTACATGATGCCAACAGAGGGTGATGATTCATCTAAAAGCGT
  CCCTTTAGCATTGCAGAGAGTGTTCTATGAGTTACAGCACAGCGATAAGCCTGTAGGAACAAAAAAGCTC
  ACAAAGTCATTTGGGTGGGAAACTTTAGATAGCTTCATGCAACACGATGTTCAAGAACTTTGTCGAGTGT
  TGCTTGATAATGTGGAAAATAAAATGAAAGGCACATGTGTAGAAGGCACCATTCCCAAGTTATTCCGAGG
  CAAAATGGTGTCTTATATTCAGTGCAAAGAAGTAGACTACCGTTCTGATAGGAGAGAAGATTATTATGAT
  ATCCAGCTAAGTATAAAAGGAAAGAAAAACATATTTGAATCATTTGTGGATTATGTGGCAGTAGAACAAC
  TAGACGGTGACAATAAATACGACGCTGGGGAGCATGGCCTGCAGGAAGCAGAGAAGGGCGTGAAGTTCCT
  GACCTTGCCCCCAGTCTTACACCTGCAGCTCATGCGGTTCATGTACGACCCTCAGACGGACCAAAACATC
  AAGATCAATGACAGGTTTGAGTTTCCAGAACAGTTACCACTTGATGAATTTTTGCAAAAAACGGACCCTA
  AGGACCCTGCAAATTATATTCTCCATGCAGTCTTGGTTCACAGTGGAGATAATCATGGTGGACATTACGT
  GGTTTACCTAAACCCCAAAGGGGATGGCAAATGGTGTAAATTTGATGATGATGTGGTATCCAGGTGTACT
  AAAGAAGAAGCAATCGAGCACAATTATGGGGGTCATGATGACGATCTATCTGTTCGACACTGCACAAATG
  CCTATATGTTAGTGTACATCAGGGAATCAAAGCTGAGTGAGGTGTTGCAAGCTGTCACCGACCATGATAT
  TCCTCAGCAGTTGGTGGAACGTTTGCAAGAAGAGAAAAGGATCGAGGCTCAGAAGCGGAAGGAGCGGCAG
  GAAGCCCATCTCTACATGCAAGTGCAGATAGTTGCAGAGGACCAGTTTTGTGGCCACCAAGGAAATGACA
  TGTACGACGAGGAGAAAGTGAGGTACACCGTGTTCAAGGTTCTGAAGAACTCCTCCCTGGCCGAGTTTGT
  TCAGAGCCTCTCGCAGACCATGGGGTTTCCACAAGATCAGATTCGATTATGGCCTATGCAAGCAAGGAGT
  AACGGGACAAAGCGGCCTGCAATGTTAGATAATGAAGCCGACGGCAGCAAAACAATGATTGAACTGAGTG
  ATAATGAAAACCCTTGGACAATATTCCTGGAAACAGTTGACCCTGAGCTGGCTGCTAGTGGAGCAACACT
  GCCCAAGTTTGATAAAGATCATGATGTAATGTTATTTTTGAAGATGTATGATCCTAAAACACGGAGCTTG
  AATTACTGTGGACACATCTACACACCCATATCCTGTAAAATACGTGACTTGCTCCCGGTTATGTGTGACA
  GAGCAGGATTTATCCAGGATACTAGCCTTATCCTCTATGAGGAAGTTAAGCCGAATTTAACAGAGAGAAT
  TCAGGACTATGATGTGTCTCTTGATAAAGCCCTCGATGAGCTGATGGATGGTGACATCATCGTGTTTCAG
  AAGGATGACCCGGAAAACGATAACAGTGAGCTACCCACTGCTAAGGAGTACTTCAGAGACCTCTACCATC
  GCGTCGACGTCATCTTCTGTGACAAAACGATCCCTAATGACCCTGGGTTTGTGGTCACCTTATCAAATAG
  GATGAATTATTTTCAGGTTGCAAAGACAGTTGCACAGAGGCTCAACACAGACCCCATGTTGCTTCAGTTT
  TTCAAGTCTCAAGGTTATAGGGACGGCCCCGGTAATCCCCTTAGGCATAATTACGAAGGTACTTTAAGAG
  ATCTTCTACAATTCTTCAAGCCAAGACAACCTAAGAAACTTTACTATCAGCAGCTTAAGATGAAAATCAC
  GGACTTTGAGAACAGGAGAAGTTTTAAGTGTATATGGTTAAACAGCCAATTTAGGGAAGAGGAAATAACA
  CTATACCCAGACAAGCATGGCTGTGTCCGGGACCTGTTAGAAGAATGTAAAAAAGCCGTGGAGCTTGGTG
  ACGAAGCATCAGGGAGACTTAGGCTGCTAGAAATTGTGAGCTACAAAATTATTGGTGTGCACCAAGAAGA
  TGAACTATTAGAGTGCTTATCTCCTGCAACGAGTCGGACTTTTAGAATAGAGGAAATACCTTTGGACCAG
  GTAAACATCGATAAGGAAAACGAGATGCTAATCACGGTGGCGCATTTCCACAAAGAAGTGTTTGGGACAT
  TCGGAATTCCGTTTTTGCTGAGGATACACCAGGGTGAGCATTTCAGAGAAGTGATGAAGCGGATTCAGAG
  CCTGCTGGATATCCAGGAAAAGGAGTTTGAAAAGTTTAAATTCGCAATTGTCATGATGGGCCGGCACCAG
  TACATCAATGAAGATGAGTATGAAGTGAATTTGAAGGACTTTGAGCCACAACCTGGTAACATGTCTCATC
  CTCGGCCTTGGCTAGGGCTTGACCACTTCAACAAAGCCCCAAAGAGGAGTCGCTACACTTACCTAGAAAA
  GGCAATTAAAATCCACAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213504 representing NM_001024790
  Red=Cloning site Green=Tags(s)
  
  MNHQQQQQQQQKAGEQQLSEPEDMEMEAGDTDDPPRITQNPVINGNVALSDGHSNAEEDMEDDTSWRSEA
  TFQFTVERFSRLSESVLSPPCFVRNLPWKIMVMPRFYPDRPHQKSVGFFLQCNAESDSTSWSCHAQAVLK
  IINYRDDDKSFSRRISHLFFHKENDWGFSNFMAWSEVTDPEKGFIDDDKVTFEVFVQADAPHGVAWDSKK
  HTGYVGLKNQGATCYMNSLLQTLFFTNQLRKAVYMMPTEGDDSSKSVPLALQRVFYELQHSDKPVGTKKL
  TKSFGWETLDSFMQHDVQELCRVLLDNVENKMKGTCVEGTIPKLFRGKMVSYIQCKEVDYRSDRREDYYD
  IQLSIKGKKNIFESFVDYVAVEQLDGDNKYDAGEHGLQEAEKGVKFLTLPPVLHLQLMRFMYDPQTDQNI
  KINDRFEFPEQLPLDEFLQKTDPKDPANYILHAVLVHSGDNHGGHYVVYLNPKGDGKWCKFDDDVVSRCT
  KEEAIEHNYGGHDDDLSVRHCTNAYMLVYIRESKLSEVLQAVTDHDIPQQLVERLQEEKRIEAQKRKERQ
  EAHLYMQVQIVAEDQFCGHQGNDMYDEEKVRYTVFKVLKNSSLAEFVQSLSQTMGFPQDQIRLWPMQARS
  NGTKRPAMLDNEADGSKTMIELSDNENPWTIFLETVDPELAASGATLPKFDKDHDVMLFLKMYDPKTRSL
  NYCGHIYTPISCKIRDLLPVMCDRAGFIQDTSLILYEEVKPNLTERIQDYDVSLDKALDELMDGDIIVFQ
  KDDPENDNSELPTAKEYFRDLYHRVDVIFCDKTIPNDPGFVVTLSNRMNYFQVAKTVAQRLNTDPMLLQF
  FKSQGYRDGPGNPLRHNYEGTLRDLLQFFKPRQPKKLYYQQLKMKITDFENRRSFKCIWLNSQFREEEIT
  LYPDKHGCVRDLLEECKKAVELGDEASGRLRLLEIVSYKIIGVHQEDELLECLSPATSRTFRIEEIPLDQ
  VNIDKENEMLITVAHFHKEVFGTFGIPFLLRIHQGEHFREVMKRIQSLLDIQEKEFEKFKFAIVMMGRHQ
  YINEDEYEVNLKDFEPQPGNMSHPRPWLGLDHFNKAPKRSRYTYLEKAIKIHN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024790
• ORF Size: 3309 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024790.1, NP_001019961.1
• RefSeq Size: 3312 bp
• RefSeq ORF: 3312 bp
• Locus ID: 360471
• Cytogenetics: 10q12
• MW: 128.4 kDa