Akr1d1 (NM_138884) Rat Tagged ORF Clone

CAT#: RR213775

Akr1d1 (Myc-DDK-tagged ORF) - Rat aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase) (Akr1d1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Akr1d1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213775 representing NM_138884
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCTCAGCACTGCAAACCACCACATACCCCTAAATGATGGTAACAGCATTCCGATCATCGGGCTTG
  GAACCTACTCAGACCCTAGACCGGTACCTGGCAAGACCTTTATAGCAGTGAAGACAGCTATTGACGAGGG
  GTACCGGCATATTGATGGGGCCTACGTCTACCGAAATGAACATGAAGTCGGTGAGGCCATCAGAGAAAAG
  GTGGCAGAAGGGAAGGTAAAGAGGGAAGAGATTTTCTACTGTGGAAAGTTATGGAGTACAGACCATGATC
  CAGAGATGGTCCGCCCAGCCCTGGAAAGGACCCTGCAGACCCTCAAGCTAGATTACATAGACCTTTATAT
  CATTGAGATGCCCATGGCCTTTAAGCCTGGAGAGGAATTTTATCCTAAAGATGAGAATGGCCGAGTGATA
  TACCACAAATCAAATCTGTGTGCCACGTGGGAGGCACTGGAAGCTTGCAAAGACGCTGGCTTGGTGAAAT
  CCCTTGGGGTGTCTAATTTTAACCGCAGGCAGCTGGAGGTCATCTTGAACAAGCCAGGACTCAAGTACAA
  GCCTGTCACCAACCAGGTGGAGTGCCACCCGTATTTCACCCAGACAAAACTCCTTGAAGTTTCTGCCAGC
  AGCATGACATCGTTCATTGTCGCATACAGCCCCTTAGGGACCTGTCGCAACCCGTTATGGGTGAATGTAT
  CTTCTCCACCCTTGTTAAAGGATGAACTCCTAACCTCGCTGGGGAAAAAGTACAATAAGACACAAGCTCA
  AATTGTGTTGCGTTTCGACATCCAGCGAGGGTTGGTTGTCATCCCTAAAAGTACTACCCCTGAAAGGATC
  AAAGAAAACTTTCAGATCTTTGACTTCTCTCTCACCAAAGAAGAAATGAAGGACATTGAAGCCTTGAATA
  AAAACGTGCGCTTTGTGGAGATGCTCATGTGGAGTGATCATCCTGAATACCCATTTCATGACGAATAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213775 representing NM_138884
  Red=Cloning site Green=Tags(s)
  
  MNLSTANHHIPLNDGNSIPIIGLGTYSDPRPVPGKTFIAVKTAIDEGYRHIDGAYVYRNEHEVGEAIREK
  VAEGKVKREEIFYCGKLWSTDHDPEMVRPALERTLQTLKLDYIDLYIIEMPMAFKPGEEFYPKDENGRVI
  YHKSNLCATWEALEACKDAGLVKSLGVSNFNRRQLEVILNKPGLKYKPVTNQVECHPYFTQTKLLEVSAS
  SMTSFIVAYSPLGTCRNPLWVNVSSPPLLKDELLTSLGKKYNKTQAQIVLRFDIQRGLVVIPKSTTPERI
  KENFQIFDFSLTKEEMKDIEALNKNVRFVEMLMWSDHPEYPFHDEY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138884
• ORF Size: 978 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_138884.1, NP_620239.1
• RefSeq Size: 3189
• RefSeq ORF: 981
• Locus ID: 192242
• MW: 37.4 kDa
• Gene Summary: enzyme that catalyzes the reduction of key intermediates during bile acid biosynthesis [RGD, Feb 2006]