Ubap2l (NM_001024798) Rat Tagged ORF Clone

CAT#: RR213776

Atp8b2 (Myc-DDK-tagged ORF) - Rat Atpase, class I, type 8B, member 2 (Atp8b2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ubap2l
• Synonyms: Atp8b2; EP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213776 representing NM_001024798
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGACGTCGGTGGGCACTAACCGAGCCCGGGGAAACTGGGAACAACCTCAAAACCAAAACCAGACAC
  AGCACAAGCAGCGGCCACAGGCCACTGCAGAGCAAATTCGACTTGCACAGATGATTTCGGACCATAATGA
  TGCTGACTTTGAGGAGAAGGTGAAACAATTGATTGATATCACAGGCAAAAACCAGGATGAATGTGTGATT
  GCTTTGCATGACTGCAATGGAGATGTCAACAGAGCCATCAATGTTCTCCTGGAAGGAAATCCAGACACGC
  ATTCCTGGGAGATGGTCGGGAAGAAGAAAGGAGTCTCAGGACAGAAGGATGGTGGACAAACTGAATCTAA
  TGAAGAAGGAAAAGAAAATCGAGACCGGGACAGAGACTATAGTCGACGACGTGGTGGGCCACCAAGACGG
  GGGAGAGGTGCCAGCCGTGGACGAGAGTTTCGGGGCCAGGAAAATGGATTGGATGGCACTAAGAGTGGTG
  GACCTTCTGGAAGAGGCACAGACAGAGGGAGAAGAGGCCGTGGCCGGGGCAGAGGTAGTTCTGGTAGACG
  AGGAGGAAGGTTTTCTGCTCAAGGAATGGGAACCTTTAACCCTGCTGATTATGCAGAGCCAGCCAACACT
  GATGATAACTATGGCAGTAGCAGTGGCAATACATGGAACAACACTGGCCACTTTGAACCAGATGATGGAA
  CAAGACTTGATTTCATTGGGGTTGAGGGGTCAAATTATCCCCGAAAATTTGAGACTGCTCCTGGTGCATG
  GAGGACTGCAACAGAAGAGTGGGGAACTGAAGATTGGAATGAAGATCTTTCTGAGACCAAGATCTTTACT
  GCCTCTAATGTGTCTTCAGTGCCTCTGCCTGCGGAGAATGTGACAATCACTGCTGGTCAGAGAATTGATC
  TTGCTGTTCTGTTGGGGAAGACACCGTCTTCAATGGAGAATGATTCATCTAATCTGGATCCATCTCAGGC
  TCCTTCCCTGGCCCAGCCTCTGGTATTCAGTAATTCGAAGCAGAATGCCATATCACAGCCTGCTTCAGGG
  AATACATTTTCTCATCATAGCATGGTGAGCATGTTGGGGAAAGGATTTGGTGATGTTGGGGAAGCTAAAG
  GTGGCAGTACAACAGGCTCTCAGTTCTTGGAGCAATTCAAGACTGCACAGGCCCTGGCTCAACTGGCAGC
  TCAACATTCTCAGTCTGGAAGCACCACCACCTCCTCTTGGGATATGGGCTCAACAACACAATCACCATCA
  CTGGTGCAGTATGATTTGAAGAGTGCAAATGATTCAACAGTGCACAGTCCTTTTACAAAGCGCCAGGCAT
  TCACCCCATCATCAACCATGATGGAGGTGTTCCTTCAGGAGAAGCCACCTGCAGTGGCTACCTCGACAGC
  TGCACCTCCTCCCCCCTCTTCTCCTCTGCCAAGCAAATCCACCTCGGCTCCACAGATGTCTCCGGGGTCT
  TCGGACAACCAATCCTCCAGCCCTCAGCCGGCTCAACAGAAACTGAAACAGCAAAAGAAGAAAACCTCCT
  TGACGTCTAAGATTCCTGCTCTGGCTGTGGAGATGCCTGGCTCAGCAGATATCTCAGGGCTAAATCTGCA
  GTTTGGGGCATTGCAATTTGGGTCAGAGCCTGTCCTTTCTGATTATGAGTCCACCCCCACCACGAGCGCC
  TCTTCAAGCCAGGCTCCCAGTAGCCTGTATACCAGCACGGCCAGTGAGTCTTCATCTACAGTTTCATCTA
  ATCAGAGTCAGGAGTCTGGTTATCAGAGTGGCCCAATTCAGTCGACAACTTATACCTCCCAAAATAATGC
  TCAGGGCCCTCTATATGAACAGAGATCCACACAGACACGGCGGTATCCCAGCTCCATCTCTTCATCACCC
  CAAAAGGACCTGACTCAGGCTAAGAATGGTTTCAGCTCTGTGCAGGCCACGCAGTTACAGACCACGCAAT
  CTGTTGAAGGTGCTACAGGCTCTGCAGTGAAATCTGAATCACCTTCCACTTCTAGTATACCCTCTCTCAA
  TGAAACAGTACCTGCAGCTTCCTTACTGACGACAGCCAGTCAGCATTCATCCTCCTTAAGTGGCTTGAGC
  CATAGTGAGGAGATTCCAAATACCACCACCACACAACATAGCAGTGCATTATCTACACAGCAGAATACCC
  TTTCTTCATCAACATCTTCTGGGCGTACTTCAACATCTACTCTTTTGCATACAAGTGTGGAGAGTGAAGC
  GAATCTGCATTCTTCCTCCAGCACTTTCTCTACCACGTCCAGCACTGTCTCTGCACCTCCCCCAGTGGTC
  AGTGTCTCCTCCAGTCTGAATAGTGGCAGTAGCCTGGGCCTTAGCCTGGGCAGCAACTCTACTGTCACAG
  CCTCAACTCGAAGCTCAGTTGCTACAACTTCAGGAAAAGCTCCTCCCAACCTTCCTCCTGGAGTCCCGCC
  ATTGTTGCCTAATCCATATATTATGGCTCCAGGGCTGTTACATGCCTACCCGCCACAAGTATATGGTTAT
  GATGATTTACAGATGCTCCAGACAAGATTTCCATTGGATTACTACAGTATCCCGTTTCCCACACCCACCA
  CTCCACTTACTGGGAGGGATGGTAGCCTGGCCAGCAACCCTTACTCTGGCGACCTCACCAAGTTCGGCCG
  CGGGGATGCCTCCTCCCCAGCCCCAGCCACAACCTTGGCCCAACCCCAACAGAACCAGACGCAGACTCAC
  CACACCACGCAGCAGACATTCCTGAACCCGGCGCTGCCTCCTGGCTATAGTTACACCAGCCTGCCATACT
  ACACAGGGGTCCCGGGCCTCCCCAGCACCTTCCAGTATGGGCCTGCTGTGTTCCCTGTGGCTCCTACCTC
  TTCCAAGCAGCATGGTGTGAATGTCAGTGTGAATGCCTCGGCCACCCCTTTCCAACAACCAAGTGGATAT
  GGGTCTCATGGATACAACACTGGTGTGTCAGTCACCTCCAGTAACACGGGCGTGCCAGATATCTCGGGTT
  CTGTGTACTCCAAAACCCAGCAGTCCTTTGAGAAACAAGGTTTTCATTCCGGTACTCCTGCTGCCTCCTT
  CAACTTGCCTTCAGCCCTAGGAAGTGGAGGGCCCATCAATCCAGCCACAGCTGCTGCCTACCCACCTGCC
  CCCTTTATGCACATTCTGACCCCACATCAGCAGCCACACTCTCAGATCCTTCACCATCACCTGCAGCAGG
  ATGGCCAGCTACCATATTTGCAGATGATTCTCTGTTGCCAGCGCCAGCAGGAGGAGCAGGATATCCTCAC
  TCTCGTCGATGACCAGCTTGGTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213776 representing NM_001024798
  Red=Cloning site Green=Tags(s)
  
  MMTSVGTNRARGNWEQPQNQNQTQHKQRPQATAEQIRLAQMISDHNDADFEEKVKQLIDITGKNQDECVI
  ALHDCNGDVNRAINVLLEGNPDTHSWEMVGKKKGVSGQKDGGQTESNEEGKENRDRDRDYSRRRGGPPRR
  GRGASRGREFRGQENGLDGTKSGGPSGRGTDRGRRGRGRGRGSSGRRGGRFSAQGMGTFNPADYAEPANT
  DDNYGSSSGNTWNNTGHFEPDDGTRLDFIGVEGSNYPRKFETAPGAWRTATEEWGTEDWNEDLSETKIFT
  ASNVSSVPLPAENVTITAGQRIDLAVLLGKTPSSMENDSSNLDPSQAPSLAQPLVFSNSKQNAISQPASG
  NTFSHHSMVSMLGKGFGDVGEAKGGSTTGSQFLEQFKTAQALAQLAAQHSQSGSTTTSSWDMGSTTQSPS
  LVQYDLKSANDSTVHSPFTKRQAFTPSSTMMEVFLQEKPPAVATSTAAPPPPSSPLPSKSTSAPQMSPGS
  SDNQSSSPQPAQQKLKQQKKKTSLTSKIPALAVEMPGSADISGLNLQFGALQFGSEPVLSDYESTPTTSA
  SSSQAPSSLYTSTASESSSTVSSNQSQESGYQSGPIQSTTYTSQNNAQGPLYEQRSTQTRRYPSSISSSP
  QKDLTQAKNGFSSVQATQLQTTQSVEGATGSAVKSESPSTSSIPSLNETVPAASLLTTASQHSSSLSGLS
  HSEEIPNTTTTQHSSALSTQQNTLSSSTSSGRTSTSTLLHTSVESEANLHSSSSTFSTTSSTVSAPPPVV
  SVSSSLNSGSSLGLSLGSNSTVTASTRSSVATTSGKAPPNLPPGVPPLLPNPYIMAPGLLHAYPPQVYGY
  DDLQMLQTRFPLDYYSIPFPTPTTPLTGRDGSLASNPYSGDLTKFGRGDASSPAPATTLAQPQQNQTQTH
  HTTQQTFLNPALPPGYSYTSLPYYTGVPGLPSTFQYGPAVFPVAPTSSKQHGVNVSVNASATPFQQPSGY
  GSHGYNTGVSVTSSNTGVPDISGSVYSKTQQSFEKQGFHSGTPAASFNLPSALGSGGPINPATAAAYPPA
  PFMHILTPHQQPHSQILHHHLQQDGQLPYLQMILCCQRQQEEQDILTLVDDQLGE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024798
• ORF Size: 3315 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024798.2, NP_001019969.2
• RefSeq Size: 4088 bp
• RefSeq ORF: 3318 bp
• Locus ID: 361984
• Cytogenetics: 2q34
• MW: 116.9 kDa