Pld1 (NM_030992) Rat Tagged ORF Clone

CAT#: RR213792

Pld1 (Myc-DDK-tagged ORF) - Rat phospholipase D1 (Pld1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pld1
• Synonyms: Pld1a; Pld1b; Plda; Pldb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213792 representing NM_030992
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGTCACTAAGAAGTGAGGCCCGGGTGAACACTTCTACACTGCAGAAGATCGCCGCAGATATGAGAAACC
  TGATAGAAAATCTCGACACCCGGGAACTTCATTTTGAGGGAGAGGAGGTGGAGTATGATGCATCTCCAGG
  AGACCCCACGGCTCAGGAAGCGTGCATCCCATTCTCTTCTATTTACAACACTCAAGGATTTAAGGAGCCC
  AACATCCAGATCTACCTCTCTGGCTGTCCTGTAAAAGCACAAGTACTGGAAGTGGAGCGCTTCACGTCGA
  CCTCGAGGATGCCAAGTGTCAACCTGTACACCATCGAGTTAACACACGGGGAATTCACATGGCAAGTTAA
  GAGGAAATTCAAGCACTTCCAAGAGTTTCATAGAGAGCTCCTCAAGTACAAAGCCTTCATCCGGATCCCC
  ATTCCCACCAAAAGACATACGTTTAGAAGACAAAATGTCAAAGAAGAGCCCAGAGAAATGCCCAGTCTGC
  CCCGTTCATCAGAAAATGCAATCCAGGAGGAGCAGTTCTTTGGCAGGAGGAAGCAACTGGAAGATTACTT
  GACAAAAATACTAAAAATGCCCATGTACAGAAACTATCATGCCACGACAGAGTTCCTTGATGTAAGCCAA
  CTGTCTTTTATCCATGATTTGGGACCAAAGGGCCTGGAAGGAATGATCATGAAAAGATCTGGGGGACACA
  GGATACCAGGTGTGAATTGCTGTGGCCATGGAAGAGCCTGCTACCGGTGGTCAAAGAGGTGGTTGATAGT
  GAAAGACTCTTTCCTGCTGTATATGAAACCAGACAGCGGGGCGATCGCCTTCGTCCTTCTTGTAGACAAA
  GAATTCAGAATCAAAGTGGGAAAGAAGGAGACAGAAACGAAATATGGACTTCGAATTGATAATCTTTCAA
  GGACACTGATTTTAAAATGTAACAGCTACAGACATGCCCGGTGGTGGGGAGGCGCCATCGAGGAGTTCAT
  CCAGAAGCACGGCACTGACTTCCTCAAAGACCATCGCTTCGGCTCCTACGCTGCGGTCCACGAGAACATT
  TTAGCTAAATGGTACGTGAACGCCAAAGGTTACTTTGAAGATATCGCAAACGCAATGGAAGGGGCAACGG
  AAGAGATTTTTATCACAGATTGGTGGTTAAGTCCAGAAATCTTCCTGAAGCGCCCAGTGGTGGAAGGCAA
  CCGCTGGAGGCTGGACTGCATCCTCAAACGGAAAGCACAACAAGGTGTGCGGATTTTCATAATGCTTTAC
  AAAGAGGTGGAGCTAGCCCTCGGGATCAACAGCGAGTACACCAAGCGGACTTTGATGCGGCTGCACCCCA
  ACATAAAGGTGATGAGACACCCAGACCATGTGTCCTCCAGTGTGTATCTGTGGGCTCATCACGAGAAGCT
  CGTCATTATCGACCAATCGGTGGCGTTTGTGGGTGGGATTGACCTGGCCTATGGACGGTGGGATGACAAT
  GAGCACAGACTCACAGATGTGGGCAGTGTCAAGCGGGTCACCTCAGGACAGTCTCTGGGCTCTCTCACAG
  CAGCATCAGTAGAGTCTATGGAATCCTTAAGCCTCAAGGACAAACATCAATCTCATAAAAACGAGCCCGT
  CTTAAAGAGTGTCAACGATACCGACATGAAACTGAAAGGCATAGGGAAGTCCAGGAAATTCTCCAAATTT
  AGCCTCTATCGCCAACTTCACCGCCGTAATCTGCACAACTCGGACAGCATTAGCAGCGTCGACAGCGCCT
  CCAACACCGGCTCCATCCGAAGTGTGCAGACAGGAGTGGGAGAGCTCCATGGGGAGACTAGGTTTTGGCA
  TGGGAAGGATTACTGCAACTTTGTCTTCAAGGACTGGGTTCAACTGGACAAACCTTTTGCTGATTTCATC
  GACAGGTACTCCACCCCCCGGATGCCTTGGCATGACATTGGTTCTGTGGTTCACGGAAAGGCAGCTCGTG
  ACGTGGCTCGACACTTCATCCAGCGCTGGAACTTCACAAAGATTATGAAGCCAAAATATCGTTCCCTTTC
  TTATCCTTTCCTGCTCCCAAAGTCTCAAGCCACAGCGCATGAGCTGAGGTATCAGGTGCCAGGGGCCGTC
  CACGCCAAAGCGCAGTTGCTCCGATCTGCTGCTGACTGGTCTGCTGGTATAAAGCACCACGAGGAATCTA
  TCCATGCTGCCTACACCCATGTCATAGAGAACAGCAAGCACTACATCTATATTGAAAACCAATTTTTCAT
  AAGCTGCGCTGATGATAAAGTCGTGTTCAACAAGGTGGGCAATGCTATCGCCCAGAGAATCCTGAAAGCC
  CACAGGGAAGGCCAGAGATATCGAGTGTACATCGTGATACCACTGCTGCCGGGATTCGAAGGAGACATTT
  CCACAGGCGGGGGAAATGCCCTACAGGCAATAATGCACTTCAACTACAGAACCATGTGCAGAGGGGAGAG
  CTCCATCCTTGAACAGTTAAAGCCAGAGCTTGGTAACAAGTGGATAAATTACATATCATTTTGCGGTCTG
  AGAACACATGCAGAGCTGGAAGGAAACCTTGTCACTGAGCTCATCTATGTCCACAGCAAGTTGTTAATTG
  CTGATGATAACACGGTTATCATAGGTTCTGCCAACATCAATGACCGGAGCATGCTGGGAAAGCGAGACAG
  CGAAATGGCTGTCATAGTGCAGGACAGACAGACAGTCCCGTCAGTGATGGATGGAAAAGAATATCAAGCT
  GGCCGCTTCGCTCAAGGCCTTCGGCTGGAGTGCTTCAGGCTTGTCCTGGGTTATCTTTCTGACCCAAGTG
  AGGACATTCAGGATCCTGTAAGTGACAAATTCTTCAAGGAGATTTGGGTTTCAACGGCAGCTCGAAATGC
  TACCATATATGATAAGGTCTTCCGGTGCCTTCCCAATGACGAAGTGCACAATTTAATCCAGTTGCGGGAC
  TTTATAAACAAGCCTATATTGGCAAAAGAGGACCGCCTTCGTGCCGAGGAAGAACTGAGGAAGATCCGTG
  GCTTCTTGGTGCAGTTCCCCTTCTACTTCTTATCAGAAGAAAACCTGCTGCCTTCCGTTGGAACCAAGGA
  AGCCATTGTGCCCATGGAGGTTTGGACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213792 representing NM_030992
  Red=Cloning site Green=Tags(s)
  
  MSLRSEARVNTSTLQKIAADMRNLIENLDTRELHFEGEEVEYDASPGDPTAQEACIPFSSIYNTQGFKEP
  NIQIYLSGCPVKAQVLEVERFTSTSRMPSVNLYTIELTHGEFTWQVKRKFKHFQEFHRELLKYKAFIRIP
  IPTKRHTFRRQNVKEEPREMPSLPRSSENAIQEEQFFGRRKQLEDYLTKILKMPMYRNYHATTEFLDVSQ
  LSFIHDLGPKGLEGMIMKRSGGHRIPGVNCCGHGRACYRWSKRWLIVKDSFLLYMKPDSGAIAFVLLVDK
  EFRIKVGKKETETKYGLRIDNLSRTLILKCNSYRHARWWGGAIEEFIQKHGTDFLKDHRFGSYAAVHENI
  LAKWYVNAKGYFEDIANAMEGATEEIFITDWWLSPEIFLKRPVVEGNRWRLDCILKRKAQQGVRIFIMLY
  KEVELALGINSEYTKRTLMRLHPNIKVMRHPDHVSSSVYLWAHHEKLVIIDQSVAFVGGIDLAYGRWDDN
  EHRLTDVGSVKRVTSGQSLGSLTAASVESMESLSLKDKHQSHKNEPVLKSVNDTDMKLKGIGKSRKFSKF
  SLYRQLHRRNLHNSDSISSVDSASNTGSIRSVQTGVGELHGETRFWHGKDYCNFVFKDWVQLDKPFADFI
  DRYSTPRMPWHDIGSVVHGKAARDVARHFIQRWNFTKIMKPKYRSLSYPFLLPKSQATAHELRYQVPGAV
  HAKAQLLRSAADWSAGIKHHEESIHAAYTHVIENSKHYIYIENQFFISCADDKVVFNKVGNAIAQRILKA
  HREGQRYRVYIVIPLLPGFEGDISTGGGNALQAIMHFNYRTMCRGESSILEQLKPELGNKWINYISFCGL
  RTHAELEGNLVTELIYVHSKLLIADDNTVIIGSANINDRSMLGKRDSEMAVIVQDRQTVPSVMDGKEYQA
  GRFAQGLRLECFRLVLGYLSDPSEDIQDPVSDKFFKEIWVSTAARNATIYDKVFRCLPNDEVHNLIQLRD
  FINKPILAKEDRLRAEEELRKIRGFLVQFPFYFLSEENLLPSVGTKEAIVPMEVWT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030992
• ORF Size: 3108 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_030992.1, NP_112254.1
• RefSeq Size: 5170
• RefSeq ORF: 3111
• Locus ID: 25096
• MW: 119.5 kDa
• Gene Summary: ubiquitous enzyme that hydrolyzes phosphatidylcholine to phosphatidic acid (PA) and choline [RGD, Feb 2006]