Cadm1 (NM_001012201) Rat Tagged ORF Clone

CAT#: RR213821

Cadm1 (Myc-DDK-tagged ORF) - Rat cell adhesion molecule 1 (Cadm1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cadm1
• Synonyms: Igsf4a; Necl-2; sgigsf; TSLC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213821 representing NM_001012201
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGTCCTGTGCTGCCGAGCGGATCCCAGTGTGCGGCGGCAGCGGCTGTGGCGGCGGCGGCGGCGC
  CTCCAGGGCTCCGGCTCCGGCTCCTGCTGTTGCTCCTCTCGGCCGCGGCACTGATCCCCACAGGTGATGG
  GCAGAATCTGTTTACTAAGGACGTGACGGTGATTGAAGGAGAAGTTGCAACCATCAGCTGTCAAGTCAAT
  AAGAGTGATGACTCCGTGATTCAGCTACTGAACCCCAACAGGCAGACCATTTACTTCAGGGACTTCAGGC
  CTTTGAAGGACAGCAGGTTTCAGCTGCTGAATTTTTCTAGCAGTGAACTCAAAGTGTCACTGACAAATGT
  CTCAATTTCGGATGAAGGGAGGTACTTCTGCCAGCTCTACACGGACCCTCCGCAGGAGAGTTATACCACC
  ATCACAGTCCTGGTCCCTCCACGTAACTTGATGATCGATATCCAGAAAGACACGGCAGTTGAAGGGGAGG
  AGATTGAAGTCAACTGCACGGCCATGGCCAGCAAGCCGGCTACGACCATCAGGTGGTTCAAAGGGAACAA
  GGAACTCAAAGGCAAATCAGAGGTGGAAGAGTGGTCGGACATGTACACTGTGACCAGTCAGCTGATGCTG
  AAGGTGCACAAGGAGGATGATGGAGTCCCAGTGATCTGCCAGGTGGAGCACCCTGCGGTCACTGGAAACC
  TGCAGACCCAGCGGTATCTAGAAGTGCAGTATAAACCGCAAGTGCAGATCCAGATGACTTATCCTCTGCA
  AGGCCTAACCCGGGAAGGGGATGCATTCGAGTTAACGTGTGAAGCCACCGGGAAGCCCCAGCCTGTGATG
  GTAACTTGGGTGAGAGTCGATGACGAAATGCCTCAACATGCCGTTCTGTCTGGGCCGAATCTGTTCATCA
  ATAACCTAAACAAAACAGACAATGGTACTTACCGCTGTGAGGCTTCCAACACAGTGGGGAAAGCTCATTC
  GGACTATATGCTGTATGTATACGATCCCCCCACAACTATCCCTCCTCCCACAACAACCACCACCACCACT
  ACCACCACCACCACCACCACCACCATCCTTACCATCATCACAGACACCACGGCGACGACAGAACCAGCAG
  TTCACGGCCTTACTCAGTTGCCCAATTCCGCAGAAGAACTGGACAGTGAGGATCTCTCAGATTCTCGAGC
  AGGTGAAGAGGGCGCCATTGGGGCAGTGGACCACGCGGTGATTGGCGGCGTCGTAGCCGTGGTGGTGTTT
  GCCATGCTATGTTTGCTCATCATTCTGGGCCGCTATTTTGCCAGACATAAAGGTACATACTTCACTCATG
  AAGCCAAAGGAGCCGATGACGCAGCAGACGCAGACACAGCTATAATCAATGCAGAAGGAGGACAGAACAA
  CTCCGAAGAAAAGAAAGAGTACTTCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213821 representing NM_001012201
  Red=Cloning site Green=Tags(s)
  
  MASPVLPSGSQCAAAAAVAAAAAPPGLRLRLLLLLLSAAALIPTGDGQNLFTKDVTVIEGEVATISCQVN
  KSDDSVIQLLNPNRQTIYFRDFRPLKDSRFQLLNFSSSELKVSLTNVSISDEGRYFCQLYTDPPQESYTT
  ITVLVPPRNLMIDIQKDTAVEGEEIEVNCTAMASKPATTIRWFKGNKELKGKSEVEEWSDMYTVTSQLML
  KVHKEDDGVPVICQVEHPAVTGNLQTQRYLEVQYKPQVQIQMTYPLQGLTREGDAFELTCEATGKPQPVM
  VTWVRVDDEMPQHAVLSGPNLFINNLNKTDNGTYRCEASNTVGKAHSDYMLYVYDPPTTIPPPTTTTTTT
  TTTTTTTTILTIITDTTATTEPAVHGLTQLPNSAEELDSEDLSDSRAGEEGAIGAVDHAVIGGVVAVVVF
  AMLCLLIILGRYFARHKGTYFTHEAKGADDAADADTAIINAEGGQNNSEEKKEYFI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012201
• ORF Size: 1428 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012201.1, NP_001012201.1
• RefSeq Size: 1801 bp
• RefSeq ORF: 1431 bp
• Locus ID: 363058
• Cytogenetics: 8q23
• MW: 51.9 kDa