Dmgdh (NM_139102) Rat Tagged ORF Clone

CAT#: RR213839

Dmgdh (Myc-DDK-tagged ORF) - Rat dimethylglycine dehydrogenase (Dmgdh), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dmgdh
• Synonyms: Me2GlyDH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213839 representing NM_139102
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCGCCTGGGCGCGCTCAGGCTGCGGGGCCTCGCGCTCCGGAGCTCCCAGGGGCGCCCAAGCTCCG
  CCGGCCTCCGGGAAGGACAGGAAAGCCCACCCTCACCCCCAGAATGGAAGGACAGAGCAGAGACTGTGAT
  CATTGGAGGTGGCTGCGTGGGCGTGAGTCTGGCTTACCATCTGGCCAAGGCAGGGATGAGAGACGTGGTC
  CTGTTGGAGAAGTCGGAACTCACGGCTGGCTCTACCTGGCACGCAGCAGGTTTAACAACTTACTTTCACC
  CCGGGATAAACTTGAAGAAGATACATTATGACAGCATCAAACTTTACGAGAGGCTGGAAGAAGAAACTGG
  ACAGGTGGTGGGGTTCCATCAACCGGGTAGTATCAGGCTTGCGACAACTCCTGAAAGGGTGGACGAATTT
  AAATACCAAATGACGCGAACCAACTGGCATGCCACCGAGCAGTATATTATTGAGCCCGAAAAAATCCACG
  AGCTGTTCCCTTTACTCAACATGGACAAGATTTTAGCCGGACTGTATAACCCGGGAGATGGCCACATCGA
  TCCATATTCTCTGACGATGGCCCTGGCTACTGGAGCTAGGAAATATGGGGCCCTTCTAAAGTATCCTGCA
  CCAGTGACATCTCTGAAGCCCAGGCCAGATGGGACATGGGACGTGGAGACTCCGCAAGGATCTGTGAGAG
  CAAATCGAATTGTGAATGCTGCGGGATTTTGGGCTCGTGAAGTGGGTAAAATGATTGGACTAGACCATCC
  CCTCATCCCTGTGCAACATCAATATGTCATCACATCAACAATCCCAGAAGTGAAGGCTTTGAAACGAGAA
  CTCCCAGTCCTTCGTGACTTGGAAGGATCCTACTACCTCCGACAGGAAAGGGACGGGCTTTTGTTTGGTC
  CCTATGAAAGTCAGGAGAAGATGAAACTTCAAGCCTCCTGGGTCGCTCATGGAGTCCCTCCAGGCTTTGG
  GAAGGAACTTTTTGAGTCTGACTTAGACCGAATCACAGAGCACGTGGAAGCTGCCATGGAAATGGTTCCT
  GTTCTGAAAAAGGCGGACATCATCAACATCGTCAACGGTCCTATCACCTACTCTCCTGACATTCTGCCTA
  TGGTGGGGCCCCATCAGGGGGTCAGGAACTACTGGGTGGCCATTGGCTTTGGATATGGCATAATCCACGC
  CGGTGGGGTCGGGACGTACCTCAGCGACTGGATCCTGCACGGAGAACCTCCCTTTGACCTGATAGAACTG
  GATCCCAACCGCTACGGCAAGTGGACGACGACGCAGTACACCGAGGCCAAAGCCCGAGAATCATACGGGT
  TCAACAACATTGTTGGTTATCCTAAAGAGGAACGGTTTGCTGGGAGGCCAACCCAGAGAGTCAGTGGGCT
  CTACAAAATCCTGGAGTCCAAGTGTTCCATGGGTTTCCATGCAGGCTGGGAACAGCCACACTGGTTCTAC
  AAGCCCGGCCAGGACACTCAGTATAGGCCAAGTTTCCGCCGCACAAACTGGTTTGAGCCGGTGGGCTCTG
  AGTACAAACAGGTTATGCAAAGAGTTGGTGTGATTGACCTGTCTCCGTTTGGCAAGTTCAACATCAAAGG
  CCAAGATTCCACCCAGCTTCTGGATCATCTCTGTGCAAATGTCATTCCCAAGGTGGGTTTTACAAACATA
  AGTCACATGTTGACGCCCAGAGGTCGAGTGTACGCTGAGCTGACTGTTTCCCACCAATCTCCTGGGGAGT
  TCCTGTTGATTACTGGCTCTGGGTCGGAACTTCACGATCTTAGATGGATTGAAGAAGCAGCCGTCAGAGG
  TGGCTATGATGTGGAAATTCGAAACATAACCGATGAGCTTGGGGTCCTGGGAGTAGCAGGGCCATATGCA
  AGGAGAGTCCTTCAGAAGCTGACCTCAGAAGATCTCAGTGATGATGTCTTCAAGTTTCTTCAAACCAAGT
  CTTTAAAGATCTCTGACATTCCTGTCACTGCTATCAGGATCTCCTATACTGGTGAGCTGGGATGGGAGCT
  GTACCACAGACGAGAAGATTCTGCAGCCCTTTATGAGCGGATCATGAATGCGGGCCAGGAGGAGGGCATC
  GACAATTTTGGAACATATGCCCTGAATGCCTTAAGACTGGAGAAGGCTTTCCGGGCCTGGGGGTCAGAGA
  TGAACTGTGACACAAACCCTTTGGAAGCTGGGCTGGACTATTTCATCAAGCTGAACAAGCCAGCCAACTT
  CACAGGGAAGCAAGCGCTGAAGCAGATTAAAGCCAAGGGGCTGAAGCGGAGACTGGTGTGCCTCACCTTG
  GCAACAGACAATGTGGATCCAGAGGGAAATGAAAGCGTTTGGTACAAGGGCAAGGTGATTGGCAACACAA
  CATCTGGAAGCTACAGTTACAGCATCCAGAAGAGCCTGGCTTTTGCTTATGTCCCAGTTGAGCTCAGTGA
  AGTGGGACAACAAGTGGAGGTTGAACTACTGGGTAAAAATTACCCAGCAACCATTATACAGGAACCATTG
  GTACTCACAGAACCTACCAGAACCAGGCTTCAGAAAGATGGTAGGAAATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213839 representing NM_139102
  Red=Cloning site Green=Tags(s)
  
  MLRLGALRLRGLALRSSQGRPSSAGLREGQESPPSPPEWKDRAETVIIGGGCVGVSLAYHLAKAGMRDVV
  LLEKSELTAGSTWHAAGLTTYFHPGINLKKIHYDSIKLYERLEEETGQVVGFHQPGSIRLATTPERVDEF
  KYQMTRTNWHATEQYIIEPEKIHELFPLLNMDKILAGLYNPGDGHIDPYSLTMALATGARKYGALLKYPA
  PVTSLKPRPDGTWDVETPQGSVRANRIVNAAGFWAREVGKMIGLDHPLIPVQHQYVITSTIPEVKALKRE
  LPVLRDLEGSYYLRQERDGLLFGPYESQEKMKLQASWVAHGVPPGFGKELFESDLDRITEHVEAAMEMVP
  VLKKADIINIVNGPITYSPDILPMVGPHQGVRNYWVAIGFGYGIIHAGGVGTYLSDWILHGEPPFDLIEL
  DPNRYGKWTTTQYTEAKARESYGFNNIVGYPKEERFAGRPTQRVSGLYKILESKCSMGFHAGWEQPHWFY
  KPGQDTQYRPSFRRTNWFEPVGSEYKQVMQRVGVIDLSPFGKFNIKGQDSTQLLDHLCANVIPKVGFTNI
  SHMLTPRGRVYAELTVSHQSPGEFLLITGSGSELHDLRWIEEAAVRGGYDVEIRNITDELGVLGVAGPYA
  RRVLQKLTSEDLSDDVFKFLQTKSLKISDIPVTAIRISYTGELGWELYHRREDSAALYERIMNAGQEEGI
  DNFGTYALNALRLEKAFRAWGSEMNCDTNPLEAGLDYFIKLNKPANFTGKQALKQIKAKGLKRRLVCLTL
  ATDNVDPEGNESVWYKGKVIGNTTSGSYSYSIQKSLAFAYVPVELSEVGQQVEVELLGKNYPATIIQEPL
  VLTEPTRTRLQKDGRKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139102
• ORF Size: 2571 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_139102.2, NP_620802.2
• RefSeq Size: 3011
• RefSeq ORF: 2574
• Locus ID: 245961
• MW: 96 kDa
• Gene Summary: Enzyme involved in choline catabolism [RGD, Feb 2006]