St8sia1 (NM_012813) Rat Tagged ORF Clone

CAT#: RR213865

St8sia1 (Myc-DDK-tagged ORF) - Rat ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (St8sia1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: St8sia1
• Synonyms: Siat8; Siat8a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213865 representing NM_012813
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCCCTGCGGGCGGGCCCTACATACATCCAGAGGGGCCATGGCCATGCTGGCGCGGAAATTCCCGC
  GTACCCGGCTACCCGTGGGAGCCAGTGCCCTCTGTGTGGTGGTCCTCTGTTGGCTCTATGTCTTCCCTGT
  CTACCGGCTGCCCAACGAGAAGGAGATCGTGCAAGGAGTGCTGGCGCAGCGCACAGCTTGGAGGAGGAAC
  CAGACGTCAGCCAGGCTCTTCAGGAAACAAATGGAAGACTGCTGCAACCCCGCCCATCTCTTTGCAATGA
  CGAAGGTGAACTCCCCCATGGGGAAGAGCCTGTGGTATGACGGGGAGTTTCTATACTCGCTCACCATCGA
  CAACTCCACGTACTCCCTCTTCCCCCAGGCAACCCCCTTCCAGCTGCCATTGAAGAAATGTGCGGTGGTG
  GGAAACGGTGGGATTCTGAAGATGAGTGGCTGTGGCCGTCAAATAGATGAAGCAAATTTTGTCATGCGGT
  GTAACCTTCCTCCCTTGTCAAGTGAATACACCAGAGATGTGGGTTCCAAGACTCAGTTGGTGACAGCTAA
  CCCCAGCATAATTCGCCAGAGATTTGAAAACCTGCTGTGGTCCAGAAAGAAGTTTGTGGACAACATGAAG
  ATCTATAACCACAGTTACATATACATGCCTGCCTTTTCTATGAAGACGGGAACAGAGCCGTCTCTCCGTG
  TGTACTACACCCTGAAAGACGCTGGAGCCAATCAAACAGTGCTTTTTGCTAACCCCAACTTTCTTCGAAA
  CATTGGAAAGTTCTGGAAGGGTAGGGGGATCCATGCCAAGCGCCTGTCTACAGGACTCTTTTTGGTGAGT
  GCAGCCTTGGGCCTTTGTGAGGAGGTGTCCATCTACGGCTTCTGGCCCTTCTCCGTGAACATGCAAGGGG
  AACCTATCAGCCACCATTACTATGACAACGTCTTGCCCTTCTCAGGCTTCCATGCCATGCCTGAGGAGTT
  TCTTCAACTCTGGTATCTTCATAAAATGGGTGCACTGAGGATGCAACTGGACCCATGTGAGGAGCCGTCG
  CCGCCACAGCCCACTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213865 representing NM_012813
  Red=Cloning site Green=Tags(s)
  
  MSPCGRALHTSRGAMAMLARKFPRTRLPVGASALCVVVLCWLYVFPVYRLPNEKEIVQGVLAQRTAWRRN
  QTSARLFRKQMEDCCNPAHLFAMTKVNSPMGKSLWYDGEFLYSLTIDNSTYSLFPQATPFQLPLKKCAVV
  GNGGILKMSGCGRQIDEANFVMRCNLPPLSSEYTRDVGSKTQLVTANPSIIRQRFENLLWSRKKFVDNMK
  IYNHSYIYMPAFSMKTGTEPSLRVYYTLKDAGANQTVLFANPNFLRNIGKFWKGRGIHAKRLSTGLFLVS
  AALGLCEEVSIYGFWPFSVNMQGEPISHHYYDNVLPFSGFHAMPEEFLQLWYLHKMGALRMQLDPCEEPS
  PPQPTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012813
• ORF Size: 1068 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012813.2, NP_036945.2
• RefSeq Size: 1472
• RefSeq ORF: 1071
• Locus ID: 25280
• MW: 40.4 kDa
• Gene Summary: an alpha-2,8-sialyltransferase that catalyzes the biosynthesis of GT1a and GQ1b as well as GD3 [RGD, Feb 2006]