Upp1 (NM_001030025) Rat Tagged ORF Clone

CAT#: RR213897

Upp1 (Myc-DDK-tagged ORF) - Rat uridine phosphorylase 1 (Upp1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Upp1
• Synonyms: MGC124655
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213897 representing NM_001030025
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCTACAGGAACTGAAGCAAAGGACCTGGAAAACCATCACAACAGTGACTGCCTCGTCCAGCTTT
  CAAATCCAAACATAGCCACGATGAAGGAAGACGTTCTCTACCATTTCAACCTCAGCACTAGCACACACGA
  TTTCCCCGCTATGTTTGGAGATGTGAAGTTTGTGTGTGTTGGTGGAAGCTCCTCCCGGATGAGCGCCTTC
  ATCAAGTATGTGGCCGCAGAGCTGGGCCTTGACCACCCAGGGAAAGAATATCCCAACATCTGCGCAGGCA
  CTGACCGCTACGCCATGTATAAAGCCGGGCCCGTGCTGTCTGTGAGCCATGGCATGGGCATCCCTTCCAT
  TGGGATCATGTTGCATGAGCTCATCAAGATGCTGTACCATGCCAGGTGCTCCAACATCACCATCATCCGC
  ATTGGCACTTCAGGAGGGATAGGTCTGGAGCCTGGCTCTGTCGTCATCACGCAGCAGGCAGTGGATGAGT
  GCTTCAAACCAGAGTTTGAGCAGATTGTGCTGGGAAAGCGGGTGACTCGAAACACCAGCCTGGACGCGCA
  GCTGGTACAGGAGTTGATGCAGTGCTCCTCAGACCTGAACGAGTTCCCCACTGTTGTGGGTAACACCATG
  TGCACCTTGGACTTCTATGAGGGGCAAGGCCGCCTGGATGGCGCCCTCTGCTCCTACACAGAGAAGGACA
  AACAGGCCTATCTGCGTGCAGCCCATGCCGCGGGTGTCCGAAATATCGAGATGGAATCTTCAGTGTTTGC
  CACCATGTGCAGTGCCTGTGGCCTGAAAGCGGCTGTGGTGTGTGTCACTCTTCTGGACCGTCTGCAAGGG
  GATCAGATCAACACTCCCCATAATGTGCTTGTGGAATACCAGCAAAGGCCGCAGCGGCTGGTGGGCCATT
  TCATCAAGAAGCGCCTAGGGAGGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213897 representing NM_001030025
  Red=Cloning site Green=Tags(s)
  
  MAATGTEAKDLENHHNSDCLVQLSNPNIATMKEDVLYHFNLSTSTHDFPAMFGDVKFVCVGGSSSRMSAF
  IKYVAAELGLDHPGKEYPNICAGTDRYAMYKAGPVLSVSHGMGIPSIGIMLHELIKMLYHARCSNITIIR
  IGTSGGIGLEPGSVVITQQAVDECFKPEFEQIVLGKRVTRNTSLDAQLVQELMQCSSDLNEFPTVVGNTM
  CTLDFYEGQGRLDGALCSYTEKDKQAYLRAAHAAGVRNIEMESSVFATMCSACGLKAAVVCVTLLDRLQG
  DQINTPHNVLVEYQQRPQRLVGHFIKKRLGRA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001030025
• ORF Size: 936 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001030025.1, NP_001025196.1
• RefSeq Size: 1153 bp
• RefSeq ORF: 939 bp
• Locus ID: 289801
• Cytogenetics: 14q21
• MW: 34.1 kDa