Thrap3 (NM_001009693) Rat Tagged ORF Clone

CAT#: RR213946

Thrap3 (Myc-DDK-tagged ORF) - Rat thyroid hormone receptor associated protein 3 (Thrap3), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Thrap3
• Synonyms: MGC94882
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213946 representing NM_001009693
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAAAACAAACAAATCCAAGTCTGGGTCTCGCTCTTCTCGGTCAAGATCAGCATCCAGATCTCGGT
  CTCGTTCGTTTTCAAAGTCTCGATCCCGAAGCCGATCTGTCTCTCGTTCAAGGAAGCGCAGGCTGAGTTC
  TAGGTCTCGTTCCAGATCATACTCTCCAGCTCATAACAGAGAAAGGAATCACCCCAGAGTATATCAGAAT
  CGAGATTTCCGAGGTCACAACAGAGGCTACAGAAGGCCCTATTACTTCCGTGGACGAAACCGAGGCTTTT
  ATCCATGGGGCCAGTATAACCGAGGTGGCTATGGAAATTACCGTTCTAATTGGCAGAACTACCGGCAAGC
  ATACAGCCCTCGTCGGGGCCGATCCCGATCTCGGTCCCCCAAGAGAAGGTCCCCTTCACCAAGGTCCAGG
  AGCCATTCTAGGAACTCGGATAAGTCATCCTCTGACAGGTCAAGACGTTCTTCGTCCTCCCGGTCGTCCT
  CCAACCACAGCAGAGTTGAATCATCTAAACGAAAGTCTACAAAAGAGAAAAAGTCCTCTTCCAAGGATAG
  CCGGCCATCTCAGGCAGCTGGTGATAACCAGGGAGATGAGGCTAAGGAGCAGACATTTTCTGGAGGCACC
  TCTCAAGATATAAAAGGGTCTGAGAGCTCAAAGCCATGGCCAGATGCTACCACCTATGGCACTGGTTCTG
  CATCACGGGCCTCGGTTTCTGATCTGAGTCCCCGGGAGAGAAGCCCAGCTCTCAAAAGCCCCCTCCAGTC
  TGTGGTGGTTAGGCGAAGGTCACCACGCCCTAGCCCTGTGCCCAAACCTAGTCCTCCTCTTTCTAATGCA
  TCCCAGATGGGCTCATCTATGTCAGGCGGTGCTGGATATCAGTCTGGAGCACACCAAGGTCCGTTTGACC
  ATGGCTCTGGGTCTTTGAGTCCATCCAAAAAGAGCCCTGTGGGTAAGAGTCCACCAGCCACTGGCTCTGC
  ATATGGTTCATCTCAGAAAGAGGAGAGCGCTGCTTCGGGAGGAGCAGCCTACACAAAAAGGTATCTGGAA
  GAGCAGAAGACGGAGAATGGGAAGGATAAGGAGCAGAAACAAACAAATACTGATAAAGAGAAGCTGAAGG
  AGAAAGGGGGCTTCTCTGATGCTGATGTCAAAATGAAATCTGATCCATTTGCTCCCAAGACGGACACTGA
  GAAGCCCTTCCGAGGCAGCCAGTCACCCAAAAGGTATAAGCTCCGGGATGACTTTGAGAAAAAGATGGCT
  GACTTCCACAAGGAGGAGATGGATGAACAAGATAAGGACAAATGTAAGGGAAGGAAGGAACCTGAGTTTG
  ATGATGAGCCCAAATTTATATCTAAAGTCATAGCCGGTGCAAGCAAAAACCAGGAGGAAGAGAAGTCAGG
  CAAGTGGGAGAGCCTGCATGCAGGGAAGGAAAAGCAGAGGAAAGCAGAGGAGCTGGAGGAGGAGCCTTTC
  ACAGAGAGATCCCGAAAGGAGGAACGTGGAGGGTCCAAGAGGAGCGAAAGTGGGCACAGGGGCTTTGTGC
  CAGAAAAGAATTTCCGGGTGACTGCATACAAGGCGGTCCAGGAGAAAAGTTCATCGCCTCCCCCAAGGAA
  GACCTCTGAGAGCCGTGACAAGCTGGGAAACAAAGGAGACTTTTCCTCAGGGAAGTCTTCCTTTTCCATT
  ACTCGGGAGGCCCAGGTCAATGTCCGGATGGACTCCTTCGATGAGGACCTTGCACGACCCAGTGGCTTAT
  TGGCTCAGGAACGAAAGCTTTGTCGGGATCTAGTCCATAGCAACAAAAAGGAGCAGGAATTCCGTTCCAT
  TTTCCAGCACATACAGTCAGCTCAGTCGCAGCGTAGCCCCTCAGAATTGTTTGCCCAGCACATAGTGACC
  ATTGTTCATCATGTTAAAGAGCATCACTTTGGATCCTCTGGAATGACATTGCATGAGCGCTTTACTAAAT
  ACCTAAAGAGAGGGAATGAACAAGAAGCAGCTAAAACTAAGAAAAGCCCAGAGATACACAGGAGGATAGA
  CATTTCCCCCAGTACATTCAGAAAGCATGGTTTGACTCATGAGGAATTGAAAAGTCCACGGGAACCTGGC
  TACAAGGCTGAAGGAAAGTACAAAGATGATCCTGTTGATCTTCGCCTTGATATTGAACGCCGTAAAAAAC
  ATAAGGAGAGAGATCTTAAGCGAGGTAAATCAAGAGAGTCGGTGGATTCCAGAGACTCTAGCCACTCAAG
  AGAAAGATCAACTGAAAAGACAGAGAAAACCCACAAAGGATCAAAGAAGCAGAAGAAGCACCGCAGAGCA
  AGAGACCGGTCTAGGTCCTCCTCGTCCTCTTCCCAGTCATCTCACTCCTACAAAGCAGAGGAGTACCCTG
  AGGAAGCAGAGGACAGGGAGGAGAGCACCACAGGTTTTGACAAATCCAGATTGGGGACCAAAGACTTTGT
  GGGTCCGAATGAAAGAGGCGGCAGAGCTCGGGGAACCTTCCAATTTCGAGCAAGAGGGAGAGGTTGGGGC
  AGAGGAAACTATTCTGGTAACAATAACAACAACAGCAACAATGATTTCCAAAAGAGAAGCCGGGAAGAAG
  AATGGGATCCCGAGTACACACCCAAGAGCAAGAAGTATTACTTGCATGACGACCGGGAAGGCGAGGGCAG
  CGACAAGTGGATGAGCCGAGGCCGCGGCCGAGGAGCCTTCCCCCGGGGGCGGGGCCGGTTCATGTTCAGG
  AAATCCAGTACCAGCCCCAAGTGGGCCCATGACAAGTTCAGTGGGGAGGAGGGGGAGATTGAAGACGATG
  AGAGTGGGACAGAGAACAGAGAAGAAAAGGACAGTTTACAACCTTCAGCTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213946 representing NM_001009693
  Red=Cloning site Green=Tags(s)
  
  MSKTNKSKSGSRSSRSRSASRSRSRSFSKSRSRSRSVSRSRKRRLSSRSRSRSYSPAHNRERNHPRVYQN
  RDFRGHNRGYRRPYYFRGRNRGFYPWGQYNRGGYGNYRSNWQNYRQAYSPRRGRSRSRSPKRRSPSPRSR
  SHSRNSDKSSSDRSRRSSSSRSSSNHSRVESSKRKSTKEKKSSSKDSRPSQAAGDNQGDEAKEQTFSGGT
  SQDIKGSESSKPWPDATTYGTGSASRASVSDLSPRERSPALKSPLQSVVVRRRSPRPSPVPKPSPPLSNA
  SQMGSSMSGGAGYQSGAHQGPFDHGSGSLSPSKKSPVGKSPPATGSAYGSSQKEESAASGGAAYTKRYLE
  EQKTENGKDKEQKQTNTDKEKLKEKGGFSDADVKMKSDPFAPKTDTEKPFRGSQSPKRYKLRDDFEKKMA
  DFHKEEMDEQDKDKCKGRKEPEFDDEPKFISKVIAGASKNQEEEKSGKWESLHAGKEKQRKAEELEEEPF
  TERSRKEERGGSKRSESGHRGFVPEKNFRVTAYKAVQEKSSSPPPRKTSESRDKLGNKGDFSSGKSSFSI
  TREAQVNVRMDSFDEDLARPSGLLAQERKLCRDLVHSNKKEQEFRSIFQHIQSAQSQRSPSELFAQHIVT
  IVHHVKEHHFGSSGMTLHERFTKYLKRGNEQEAAKTKKSPEIHRRIDISPSTFRKHGLTHEELKSPREPG
  YKAEGKYKDDPVDLRLDIERRKKHKERDLKRGKSRESVDSRDSSHSRERSTEKTEKTHKGSKKQKKHRRA
  RDRSRSSSSSSQSSHSYKAEEYPEEAEDREESTTGFDKSRLGTKDFVGPNERGGRARGTFQFRARGRGWG
  RGNYSGNNNNNSNNDFQKRSREEEWDPEYTPKSKKYYLHDDREGEGSDKWMSRGRGRGAFPRGRGRFMFR
  KSSTSPKWAHDKFSGEEGEIEDDESGTENREEKDSLQPSAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009693
• ORF Size: 2853 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009693.1, NP_001009693.1
• RefSeq Size: 3586 bp
• RefSeq ORF: 2856 bp
• Locus ID: 313591
• Cytogenetics: 5q36
• MW: 108.3 kDa