Cyp8b1 (NM_031241) Rat Tagged ORF Clone

CAT#: RR213991

Cyp8b1 (Myc-DDK-tagged ORF) - Rat cytochrome P450, family 8, subfamily b, polypeptide 1 (Cyp8b1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyp8b1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213991 representing NM_031241
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTGGGGTTCAGTGCTAGGGGCCCTGCTCATGGCTGTCGGTTGCCTCTGCCTCTCCTTGCTGCCCA
  GGCACCGCAGGCCCTGGGAGCCACCTCTGGACAAGGGGTTTGTGCCCTGGCTGGGTCACACCATGGCCTT
  CCGGAAGAATATGTTTGAATTCCTGAAGGGAATGCGGGCCAAGCACGGGGACGTGTTCACACTGCAGCTG
  GGAGGCCAGTACTTCACCTTTGTCATGGATCCCCTCTCCTTTGGCCCCATCATTAAGAGCACGCAGAAAG
  TGCTAGACTTCGTGACGTACGCTCGGGAACTGGTGTTCAAGGTGTTTGGGTACCAGTCCATGGATGAGGA
  CCACCAGATGCTGCACGTAGCCAGTACCAAGCATCTGATGGGACAAGGCTTGGAGGACCTCAACCGGGCC
  ATGCTGGACAGCCTGTCCTTGGTGATGCTAGGACCCAAAGGCCGGAGTCTGGGTGCCAGGTCCTGGTGTG
  AGGACGGCCTCTTCCACTTCTGCTACAGCATCTTGTTTAAGGCTGGCTTCCTGAGCTTGTTTGGCTGCAC
  CAAGGACAAGGAGCAGGACCTGGATGAGGCCGATGAGCTGTTCAGGAAGTTCCGCAGATTTGACCTACTT
  TTCCCCAGGTTTGTCTACTCCCTGCTGGGGCCCCTGGAGTGGGTAGAAGTGAGCCAGCTGCAGCGTCTCT
  TCCACCAGAGACTCTCTGTGGAGCAAAACCTGGAGAAAGACGGCATAAGTAACTGGTTGGGCTTCATGCT
  TCGGTTTCTGAGGGAGCGAGGGATGGCTTCATCCATGCAGGACAAATTTAACTTTATGATGCTCTGGGCC
  TCCCAGGGGAACACGGGGCCCACCTGTTTCTGGGCCCTCCTGTTCCTGCTGAAGCATCAGGACGCGATGA
  AGGCTGTGCGAGAGGAAGCCACTCGGGTCCTGGGTGAGGCCAGGTTGGAAGCCGAGACATCCTTCGCTTT
  CACTCTCAGTGCCCTGAAATGCACTCCAGTGCTGGACAGCGTGATGGAAGAGACTCTTCGTCTCTGTGCC
  ACCCCCACCCTCCTCGGGGTGGTGCAGGAGGATTATGTCCTAAAGATGGCCAGTGGGCAAGAGTACCAGA
  TCCGCCGTGGAGATAAGGTGGCTCTCTTCCCCTATCTCTCAGTACACATGGACCCCGACATCCATCCCGA
  GCCCACGACCTTCAAGTACAATCGGTTCCTCAACCCTGATGGCACCCGGAAAGTGGACTTCTACAAGTCA
  GGCAAGAAGATCCACCACTACAACATGCCCTGGGGGTCGGGTGTCTCCATATGTCCCGGCAGGTTCTTTG
  CCCCCAGTGAGATGAAGACCTTTGTCCTCCTTATGGTCATGTACTTTGACTTTGAACTGGTGGACCCTGA
  CATGCCTGTGCCCCCTATCGACCCAAGGCGCTGGGGCTTCGGCACCTCCCAGCCCAGCCATGAGGTACGC
  TTTCGCTACCGCCTGAAGCCCATGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213991 representing NM_031241
  Red=Cloning site Green=Tags(s)
  
  MLWGSVLGALLMAVGCLCLSLLPRHRRPWEPPLDKGFVPWLGHTMAFRKNMFEFLKGMRAKHGDVFTLQL
  GGQYFTFVMDPLSFGPIIKSTQKVLDFVTYARELVFKVFGYQSMDEDHQMLHVASTKHLMGQGLEDLNRA
  MLDSLSLVMLGPKGRSLGARSWCEDGLFHFCYSILFKAGFLSLFGCTKDKEQDLDEADELFRKFRRFDLL
  FPRFVYSLLGPLEWVEVSQLQRLFHQRLSVEQNLEKDGISNWLGFMLRFLRERGMASSMQDKFNFMMLWA
  SQGNTGPTCFWALLFLLKHQDAMKAVREEATRVLGEARLEAETSFAFTLSALKCTPVLDSVMEETLRLCA
  TPTLLGVVQEDYVLKMASGQEYQIRRGDKVALFPYLSVHMDPDIHPEPTTFKYNRFLNPDGTRKVDFYKS
  GKKIHHYNMPWGSGVSICPGRFFAPSEMKTFVLLMVMYFDFELVDPDMPVPPIDPRRWGFGTSQPSHEVR
  FRYRLKPMQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031241
• ORF Size: 1497 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031241.1, NP_112520.1
• RefSeq Size: 1965
• RefSeq ORF: 1500
• Locus ID: 81924
• MW: 57.6 kDa
• Gene Summary: member of the cytochrome P450 enzyme superfamily; endoplasmic reticulum membrane protein; plays a role in sterol metabolism; human homolog is unique among cytochrome P450 genes in that it is intronless [RGD, Feb 2006]