Lrrc36 (NM_001004088) Rat Tagged ORF Clone

CAT#: RR214036

Lrrc36 (Myc-DDK-tagged ORF) - Rat leucine rich repeat containing 36 (Lrrc36), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lrrc36
• Synonyms: Xlhsrf2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214036 representing NM_001004088
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCAATGGGATCTGGACGAGGAGTGCCTCCGCCGCCTGGGGGCGCTGACCCTCGAGCAGCCGG
  AACTGGTGGAGTCTCTCTCATTGCAGGGATCTTATGCTGGAAAAATCCACTCTATTGGTGATGCCTTCAG
  AAATTTCAAAAATCTCCGATCCTTAGATTTATCAAGGAATTTGATCACTAGTCTGAAGGGCATCCAGTAT
  CTATGTTCACTCCAAGATCTGAACTTATATTATAACAACATTCCTTCATTAGTGGAGGTGTCCCGACTAC
  AGCCCTTACCCTTCCTCAAAGAACTAGATTTGAGACTCAATCCTGTTGTAAGGAAAGATACAGATTATAG
  GCTCTTTGCTGTGTACACACTGCAAATGCTGGAGAAACTGGATGACCGAGCTGTCCGTGACAGTGAGAGG
  AGAGCGGCCAAGCTACATTTCAGTCAGTTGGGCAGCAGTGAAAATTTTCTCCTAGAGGTGGAAAAAAGCT
  CTAGGGAGAAGACAATGAAAAACTGTGTGACAGATGAGGGCCCTGCATCACACGTCAGTTCTGAGGTTGA
  CACCAGAATGGAAACAGACTCAAACAAAGGACTTTTTATTTCCTTCCCCAACCGGGAAATAAAGGATTCC
  CTAGCAAGCATTTGTGCAACCCAGGGCAGTGGCACACCAGCTCAGAAATTAGACGTTTTTCCACTGGAGA
  CACAGATGCAGGAAGCAACAAGAAGGGAGACAAGTGACCTCCACCAGGAAGATGGATTGGAACTCAGGCA
  TTACTCGCCTCATCAGGGCACAGTCCGATCTCCAGAGAAGATGACAAGAGATGGATACCGAGTATCTTTT
  TTAGACACTAAGTCTTCAGGTTCTTCTCCAGTAAAGGACTTGATACCAAAACCTGATGCCTATCCGTGTA
  CCCATGATGCCTCTTTGGGTAAACGTCTGGATGTGGGTGATTCTAACCAGATCCTTCCCTGCCAATTACC
  TTCTGGTGTTTGTCTGGACCATTATGATAATCATTATTCTCAAACTCTTTGCCTGCACGGAAGTCTTGTC
  AAAAGGCCTCAAAAAGGCAAGTACTATCGAGAATACAGCACAAAGCCTTCCAATGATAAAAAGGCCGCTT
  CATCCCTTTCCTGTGGAGACCTGCTAACATCCCTGTCAAACCCTGACTGCAGCACAGGAAGGCCATTGAA
  GCTCAGTTCAGATCTCTATGCCACAACCCGTTTCAACAGTGACCATGCTCTGCTGGCCAATGTAGAGCAA
  CAGCTGTCCAGCCTCGGTGATTTTGCACCAGCACCTGGTTCCTTCCCAAGCAACCCTGCCCTAGGAAATT
  CTCTGCGGACACTGCTGTTGCCTCCTGGGACTCCAGAAAACAGAGAGATTCCAACTAAGAGGTCATTAAG
  CCCTTCAAGGAGAGGATTCAAGCGGAAGGACAATATCCTTGCCAACCTGAATCTGAAACGTGGTTTCCAA
  GATGCTACAGGCAGCGAGGTGCAACCTTTCTCTAGTGACCTGGGCAGTTTGCATGGTTTATCAGGAAACC
  GTAGTCCCCCGATCTCTGCCAGAACACCCCACGTAGCCACTGTCCTCAGACAGCTCCTGGAGCTGGTGGA
  TAAGCACTGGAATGGTTCTGGCTCCCTTCTCCTCAACAAGAAGTTTCTTGGTCCTGCCAGAGATTTGCTC
  CTGTCCTTGGTGGTGCCTGCACCTCCCCAGCAGTGGCATCGCTCGCTGCTTGACGACAAGACAGGGAGAG
  CCTTGCACTGGAGGGAGACGGAGCTAAAGGAAGCAGGGCAGCTGGTTCCCAGTGATGTGGAAAGTTTGAA
  GCAAAAACTGGTCAAAGTGTTGGAAGAAAACCTTATTTTGTCAGAAAAAATTCAGCAGTTGGAGGGAGCT
  GCTGCCACCTCGGTTGTGAGTGGGCACCCATCTCACACACACGATGATCTTCTGCACAAAAACCAACAGC
  TGACCATACAGGTGGCTTGCCTGAACCAGGAGCTGACTCAGCTGAAAAGGCTGGAGGAGACAGTTGCTCT
  TCTCCATGAGAGTCAGAGGTCCCTGGTGGTAACTAATGAGTATCTGCTGCAGCAGCTGAATAAAGAGCAG
  AAAGGTTATTCCGGGAAATCGCTCCTGCCTCCTGAGAAGACTCATCCTTTGGGGAGATTGTCGCCCTTTG
  GGAAAAGCACTCTGTCTTCCTCCTCACCAACGGTACACAACACGGGTCAGTACCTAATACAGAGTGTCTC
  AGAAGCTGACCCTGAGCCTAGTCTGTGGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214036 representing NM_001004088
  Red=Cloning site Green=Tags(s)
  
  MAEQWDLDEECLRRLGALTLEQPELVESLSLQGSYAGKIHSIGDAFRNFKNLRSLDLSRNLITSLKGIQY
  LCSLQDLNLYYNNIPSLVEVSRLQPLPFLKELDLRLNPVVRKDTDYRLFAVYTLQMLEKLDDRAVRDSER
  RAAKLHFSQLGSSENFLLEVEKSSREKTMKNCVTDEGPASHVSSEVDTRMETDSNKGLFISFPNREIKDS
  LASICATQGSGTPAQKLDVFPLETQMQEATRRETSDLHQEDGLELRHYSPHQGTVRSPEKMTRDGYRVSF
  LDTKSSGSSPVKDLIPKPDAYPCTHDASLGKRLDVGDSNQILPCQLPSGVCLDHYDNHYSQTLCLHGSLV
  KRPQKGKYYREYSTKPSNDKKAASSLSCGDLLTSLSNPDCSTGRPLKLSSDLYATTRFNSDHALLANVEQ
  QLSSLGDFAPAPGSFPSNPALGNSLRTLLLPPGTPENREIPTKRSLSPSRRGFKRKDNILANLNLKRGFQ
  DATGSEVQPFSSDLGSLHGLSGNRSPPISARTPHVATVLRQLLELVDKHWNGSGSLLLNKKFLGPARDLL
  LSLVVPAPPQQWHRSLLDDKTGRALHWRETELKEAGQLVPSDVESLKQKLVKVLEENLILSEKIQQLEGA
  AATSVVSGHPSHTHDDLLHKNQQLTIQVACLNQELTQLKRLEETVALLHESQRSLVVTNEYLLQQLNKEQ
  KGYSGKSLLPPEKTHPLGRLSPFGKSTLSSSSPTVHNTGQYLIQSVSEADPEPSLWS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004088
• ORF Size: 2271 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004088.2, NM_001004088.3, NP_001004088.3
• RefSeq Size: 2410 bp
• RefSeq ORF: 2274 bp
• Locus ID: 361394
• Cytogenetics: 19q12
• MW: 84.1 kDa