Hibadh (NM_022243) Rat Tagged ORF Clone

CAT#: RR214073

Hibadh (Myc-DDK-tagged ORF) - Rat 3-hydroxyisobutyrate dehydrogenase (Hibadh), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hibadh
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214073 representing NM_022243
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCTCCTTGGGGTTCCGGGGGGCAGCCTCCGGCCTCCGGTACTGGAGCGGCCGGCGGCGGCCCG
  TGGGCAGCCTTGCAGCGGTGTGTTCTAGGTCAATGGCTTCTAAGACTCCAGTTGGATTCATTGGACTGGG
  AAACATGGGCAATCCAATGGCAAAAAATCTCATAAAACATGGCTACCCACTCATCCTCTATGATGTGTTC
  CCTGACGTATGCAAAGAGTTTAAAGAGGCTGGTGAACAGGTAGCGTCCTCCCCAGCAGATGTAGCGGAAA
  AGGCTGACAGGATTATCACAATGCTGCCCTCCAGTATGAATTCAATAGAAGTTTATTCTGGAGCAAATGG
  AATTCTAAAAAAAGTGAAGAAAGGCTCATTATTAATAGATTCCAGTACTATTGATCCTTCAGTTTCAAAA
  GAATTGGCAAAAGAAGTTGAGAAAATGGGAGCTGTTTTCATGGACGCCCCTGTTTCTGGTGGCGTAGGAG
  CTGCTCGGTCTGGAAACCTTACATTCATGGTGGGAGGAGTTGAAAATGAGTTTGCTGCTGCCCAAGAGTT
  GCTGGGGTGCATGGGCTCCAATGTATTGTACTGTGGAGCTGTCGGGTCTGGGCAGTCTGCAAAGATCTGT
  AACAACATGCTGTTGGCTATAAGTATGATCGGAACTGCTGAAGCTATGAACCTTGGAATCAGGTCAGGCC
  TTGACCCAAAACTATTGGCTAAAATCCTGAATATGAGCTCAGGACGATGCTGGTCCAGCGACACTTACAA
  CCCTGTACCTGGGGTTATGGATGGAGTTCCCTCATCTAATAACTACCAAGGTGGATTTGGGACAACACTC
  ATGGCCAAGGATCTGGGATTAGCTCAAGACTCTGCCACCAGCACCAAGACCCCAATTCTCCTCGGAAGCG
  TGGCCCATCAGATATACAGGATGATGTGTTCAAAGGGCTACTCAAAGAAAGACTTCTCCTCTGTGTTCCA
  GTATCTGCGGGAGGAGGAAACCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214073 representing NM_022243
  Red=Cloning site Green=Tags(s)
  
  MAASLGFRGAASGLRYWSGRRRPVGSLAAVCSRSMASKTPVGFIGLGNMGNPMAKNLIKHGYPLILYDVF
  PDVCKEFKEAGEQVASSPADVAEKADRIITMLPSSMNSIEVYSGANGILKKVKKGSLLIDSSTIDPSVSK
  ELAKEVEKMGAVFMDAPVSGGVGAARSGNLTFMVGGVENEFAAAQELLGCMGSNVLYCGAVGSGQSAKIC
  NNMLLAISMIGTAEAMNLGIRSGLDPKLLAKILNMSSGRCWSSDTYNPVPGVMDGVPSSNNYQGGFGTTL
  MAKDLGLAQDSATSTKTPILLGSVAHQIYRMMCSKGYSKKDFSSVFQYLREEETF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022243
• ORF Size: 1005 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022243.1, NP_071579.1
• RefSeq Size: 1769
• RefSeq ORF: 1008
• Locus ID: 63938
• MW: 35.3 kDa
• Gene Summary: catalyzes the conversion of 3-hydroxy-2-methylpropanoate and NAD+ to 2-methyl-3-oxopropanoate, NADH, and H+ [RGD, Feb 2006]